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Kimball Et Al 2018, Figure 1
Kimball Et Al 2018, Figure 1
Abundance
Focused Targeted analysis
Expression
Broad Open-ended analysis Cellular Identity
Population Structure
High hts
-dim nsig
ensio
nal an i cipated i
alysis of unant
multiple parameters can identify
Examples:
Focused Cellular Identity Are distinct CD8 T cell phenotypes found during virus infection?
Broad Population Structure Does the distribution of cell populations differ between conditions?
B. CyTOF Algorithms
82261
82241
Model Cross Validation Error Rate
82251
75
82223
0 82228
82255
82265 82263
82229
82247
82262
82267
50
82259
82268 82236 82232
82264
82266 82256
-20
tSNE2
82249 82252
25
Model:
82250
82260
Min
82230 82243
82257
82254
SE
-30 0 30
0
82239
82234
Constrained
tSNE1 4.68 4.16 3.64 3.12 2.60 2.08 1.56 1.04 0.52 0.00
Regularization Threshold
Utility:
Rapid visual analysis, In depth visual analysis, Rapid statistical analysis,
limited quantitative analysis rapid quantitative analysis limited visual analysis
Data Output:
Abundance
Expression
Cellular Identity
Population Structure
Computational skills:
Beginner Advanced Intermediate
Figure 1
Kimball et al