Professional Documents
Culture Documents
Kimball Et Al 2018, Figure 9
Kimball Et Al 2018, Figure 9
Kimball Et Al 2018, Figure 9
B6 #1
T cells in FlowJo
viSNE are not significantly different.
viSNE
**
IL10KO #1
B.
** 150
Median Intensity
***
ns
*
100
C. B6
D. Focused analysis on CD4+ T cell clusters
CD11c CTLA4
Lag3
PhenoGraph defined six
subtypes of CD4+ T cells. PhenoGraph-defined
PhenoGraph
Individual Events
B6 biased
Individual Events
Sca CI
S
PD1
6
The inner ring indicates core
ICO
IL10KO biased
X-shift
CD3
IRF4
4 CD4+ T cells
markers expressed on all
Tim3
Lag3
#515
1 L
2
44
PD
CD4+ T cells.
CD
Tb CD4 TR
Average of all events e
H.
t GI
Tbet
Tim3
Lag3
IkBa
CD19
NKp46
Ki67
PDL2
CD64
PDL1
IRF4
Sig F
FoxP3
KLRG1
Bcatenin
CD127
CD11c
GITR
MHCI
CD69
CD8
CD11b
CD25
Gr-1
p4eBP1
CD3
CTLA4
PD1
Sca1
CD45
CD44
CD4
CD117
MHCII
ICOS
CTLA4
100 Parameters
CI
Cellular expression
Median Expression
6
The outer ring defines
MHCII
Gr-1
accessory phenotypes,
showing data from
CD
2
44
11
with characteristics
Ki
CD11b
a
c
IkB
67
#1
ns
**
Median Intensity
#26 3
IL10KO #1
#1 between conditions.
2
B6
1
1.0 6.5 0.5 6.8 0.3 4.5 0.8 5.8 0.0 4.3
Median expression values IL10KO
for a single node can be 0
Selected CD4+ T cell nodes assessed for significance. CD11b CD11c Gr-1 PDL1 Tbet
K.
Number of model features #82228 IRF4 #82254 IRF4 #82228, PDL1 #82267, CD11b
I. J.
82233 82253
Median Expression
Median Expression
82256
3 3 4 6
100
3
82239
82228 82249
82254 82250
0
82258
0
Citrus
0
82267
0
O
IL 6
O
IL 6
O
IL 6
O
82268 82222
B
B
B
K
82263
K
82260
10
10
10
10
82259
IL
82266
50
82237
82251
82262
82257
#82228
#82254
#82228
#82267
82252
82247
82255
25
82245
82241
IRF4 IRF4 PDL1 CD11b
82217
82220
Population: Background Cluster
Figure 9
7.15 6.36 5.56 4.77 3.97 3.18 2.38 1.59 0.79 0.00
Regularization Threshold
differences between experimental groups. Kimball et al