1.

0.8

Absorbance at 540 nm
y = 1.8593x - 0.0475
0.6 R² = 0.9936

0.4

0.2

0.0
0 0.1 0.2 0.3 0.4 0.5
concentration of NAG (mg/ml)

Figure 3. NAG standard curve

0.8
OD at 595 nm

y = 4.8404x + 0.0315
0.6 R² = 0.9908

0.4

0.2

0
0 0.02 0.04 0.06 0.08 0.1 0.12
BSA concentration (mg/ml)

Figure 4. BSA standard curve