Lenis C, López J, Ulloa JC, et al

ARTÍCULO ORIGINAL

Genomas virales fragmentados sugieren

contaminación para aguas de consumo humano
Viral genome fragments suggests contamination
of human drinking water
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Resumen
Objetivo.'HWHUPLQDUODSUHVHQFLDGHURWDYLUXV\QRURYLUXVHQDJXDSDUDFRQVXPRKXPDQRHQXQDORFDOLGDGGH%RJRWi
Materiales y métodos.6HUHFROHFWDURQRFKRPXHVWUDVGHDJXDSRUVHPDQDHQRFKROXJDUHVGLIHUHQWHVGHOSUHGLRHQHVWXGLR3DUDGHWHUPLQDUOD
SUHVHQFLDGHOJHQRPDYLUDOODVPXHVWUDVVHXOWUDÀOWUDURQ\VHKL]RGHWHFFLyQYLUDOSRUPHGLRGHODWpFQLFD573&5DPSOLÀFDQGRXQVHJPHQWRGHO
JHQVP6GHOURWDYLUXV\XQVHJPHQWRGHOORF2GHOQRURYLUXV(VWHHVWXGLRVHOOHYyDFDERHQXQDLQVWLWXFLyQXQLYHUVLWDULDXELFDGDHQXQD]RQDSH-
ULXUEDQDFHUFDQDD%RJRWiDNPGHODFXHGXFWRTXHVXUWHGHDJXDDOSUHGLR/D]RQDVHHQFXHQWUDURGHDGDSRUÀQFDVFRQJDQDGROHFKHURFHQWURV
FRPHUFLDOHV\FXDWURFHPHQWHULRV(ODJXDVHUHFROHFWyHQUHFLSLHQWHVGHSROLSURSLOHQR(QWRWDOIXHURQPXHVWUDVGHFLQFROLWURVGHDJXDFDGDXQD
Resultados./DSUHVHQFLDYLUDOIXHGHHQFRQWUiQGRVHURWDYLUXVHQFXDWUR\QRURYLUXVHQFXDWURGHODVPXHVWUDVFROHFWDGDV$GHPiVGH
SUHVHQWDUODLPSRUWDQFLDGHODSUHVHQFLDYLUDOHQDJXDSDUDFRQVXPRKXPDQRHQHVWHGRFXPHQWRVHGLVFXWHHOVLJQLÀFDGRTXHWLHQHHOHQFRQWUDU
VRORVHJPHQWRVYLUDOHV\QRODSDUWtFXODYLUDOFRPSOHWDHLQIHFFLRVD
Palabras clave: DJXDSRWDEOHURWDYLUXVQRURYLUXV

Abstract
Objective: 7RGHWHUPLQHWKHSUHVHQFHRIURWDYLUXVDQGQRURYLUXVLQGULQNLQJZDWHULQDQRUWKHUQQHLJKERUKRRGLQ%RJRWi&RORPELD
0DWHULDOVDQGPHWKRGV(LJKWZHHNO\VDPSOHVRIZDWHUZHUHFROOHFWHGDQGXOWUDÀOWHUHGLQRUGHUWRGHWHFWWKHYLUDOJHQRPHSUHVHQFH7KHWHFKQLTXH
XVHGIRUGHWHFWLRQRIJHQRPHVHJPHQWVZDV573&593JHQHIURPURWDYLUXVDQG25)VHJPHQWIURPQRURYLUXVZHUHVHOHFWHGLQRUGHUWRÀQG
WKHYLUXVLQWKHZDWHU7KLVVWXG\ZDVSHUIRUPHGLQDKLJKHUHGXFDWLRQLQVWLWXWLRQORFDWHGLQDSHULXUEDQ]RQHQHDU%RJRWiNPDZD\IURPWKH
DTXHGXFWWKDWVHUYLFHVWKHDUHD7KH]RQHLVVXUURXQGHGE\GDLU\FDWWOHIDUPVPDOOVDQGIRXUFHPHWHULHV:DWHUZDVFROOHFWHGLQFOHDQSRO\SURS\OHQH
FRQWDLQHUV7KHUHZHUHDWRWDORIVDPSOHVRIOLWHUVRIZDWHUHDFK
Results: 9LUDOVHJPHQWVZHUHIRXQGLQRIWKHVDPSOHV:HIRXQGURWDYLUXVLQIRXUVDPSOHVDQGQRURYLUXVLQDQRWKHU%HVLGHVGLVFXVVLQJWKHLP-
SRUWDQFHRIYLUDOFRQWDPLQDWLRQLQGULQNLQJZDWHUZHGLVFXVVHGWKHPHDQLQJRIÀQGLQJRQO\YLUDOVHJPHQWVDQGQRWFRPSOHWHDQGLQIHFWLRXVYLUDOSDUWLFOHV
Keywords: GULQNLQJZDWHUURWDYLUXVQRURYLUXV

Introducción FRPR OD JDVWURHQWHULWLV OR TXH KDFH VXSRQHU

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FXOR WUDQVPLVRU GH PLFURRUJDQLVPRV SULQFLSDO-
PHQWHDTXHOORVFX\RWDPDxRVHPLGHHQQDQy-
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GDVFRQDJXDSRWDEOHGHEDxR\GHPDU\FRQHO
FRQVXPRGHRVWUDV\DVRFLDGDVDODSUHVHQFLDGH
URWDYLUXVQRURYLUXVDVWURYLUXVDGHQRYLUXVYLUXV
GHODKHSDWLWLV$\HQWHURYLUXVGHWLSRSROLRPLH-
OLWLV&R[VDFNLH\HFKRJHQHUDQGRHQIHUPHGDGHV
TXH GHVSXpV GH OD WUDQVPLVLyQ SHUVRQD D SHU-
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FLSDOHV SUREOHPDV SDUD YHULÀFDU OD LQRFXLGDG GHO
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Materiales y métodos
Lugar del muestreo
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SXQWRSRUVHPDQDHQUHFLSLHQWHVGHSROLSURSL-
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Centriprep Ultracel YM-50MilliporeVHFHQWUL-
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Clarificación y purificación viral
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Extracción del ARN viral y determinación de
la presencia de virus en las muestras
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 Genomas virales fragmentados sugieren contaminación en aguas de consumo humano
/DV EDQGDV VH YLVXDOL]DURQ HQ JHO GH DJDUR-
VD DO   FRQ DGLFLyQ GH EURPXUR GH HWLGLR
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Resultados
Detección de virus en las muestras de agua
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muestreo
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