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PENUNTUN PRAKTIKUM
Ilmu Dasar Keperawatan
(IDK II)

Pewarnaan Bakteri

Kuntarti/IDK2/2011 1
 Pewarnaan Bakteri
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Topik : Pewarnaan Gram

Tujuan Pewarnaan :
a. Mengetahui bahwa bakteri dapat dilihat dengan
pewarnaan
b. Mengenal bakteri positif Gram dan negatif Gram
serta morfologinya

Kuntarti/IDK2/2011 2
 Faktor - faktor penentu keberhasilan Pewarnaan :
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1. Gelas alas bersih dan bebas lemak

2. Umur biakan (optimal) : 18 - 24 jam kecuali
bakteri tahan asam (M. tuberculosis)
3. Kualitas zat warna
4. Tebal tipisnya sediaan

Hasil pewarnaan :

bakteri Gram positif : ungu

bakteri Gram negatif : merah
Kuntarti/IDK2/2011 3
 Cara pembuatan sediaan
From liquid media From solid media
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Kuntarti/IDK2/2011 4
 Pewarnaan Gram
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Gently stain with crystal violet Gently stain wash off the stain Gently apply Gram’s iodine for 45 –
For 1-2 minutes With tap water 60 seconds

Counterstain with Safranin for 45

Kuntarti/IDK2/2011
Gently wash off the Safranin with 5
secons until 1 minutes tap water
 6
Staphylococcus sp.

Kuntarti/IDK2/2011
PERTUNJUKAN :

Vibrio sp.

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Escherichia coli

Kuntarti/IDK2/2011
B. subtilis

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PEWARNAAN BAKTERI TAHAN ASAM

Tujuan :

1. Mengetahui teknik pewarnaan bakteri tahan asam

2. Mampu mengidentifikasi bakteri tahan asam secara

mikroskopik

Pewarnaan yang digunakan :

a. Kinyoun Gabbet (Tan Thiam Hok)

b. Ziehl Neelsen (WHO)

Kuntarti/IDK2/2011 8
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Prinsip :

a. Fukhsin karbol

ikat (cuci dengan asam alkohol);

tahan asam (merah)

b. Fukhsin karbol

lepas (cuci dengan asam alkohol);

bukan tahan asam (biru)

Kuntarti/IDK2/2011 9
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Pewarnaan Tahan Asam

Kuntarti/IDK2/2011

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Biakan M. tuberculosis M. tuberculosis dengan

pada agar Lowenstein Jensen pewarnaan Ziehl Nelsen

Kuntarti/IDK2/2011 11
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Kuntarti/IDK2/2011

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