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Pex 12 04
Pex 12 04
Exercise 12: Serological Testing: Activity 4: Western Blotting Technique Lab Report
1. Which of the following tests is not correctly matched to what it tests for?
You correctly answered: d. These are all correctly matched.
4. In this activity you will use Western blotting to test for the presence of
Your answer : a. antigens (like the indirect ELISA).
Correct answer: b. antibodies (like the indirect ELISA).
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Experiment Results
Stop & Think Questions:
What is present on the nitrocellulose strips?
You correctly answered: a. HIV antigens separated by electrophoresis
How would you describe the binding site of the secondary antibody?
You correctly answered: c. the constant region of the primary antibody
Experiment Data:
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Post-lab Quiz Results
You scored 75% by answering 3 out of 4 questions correctly.
3. A key difference between the ELISA and the Western blot technique is that the
You correctly answered: a. Western blot looks at separated antigens.
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Review Sheet Results
1. Describe why the HIV Western blot is a more specific test than the indirect ELISA for HIV.
You did not answer this question.
2. Explain the procedure for a patient with an indeterminate HIV Western blot result.
You did not answer this question.
3. Briefly describe how the nitrocellulose strips were prepared before reacting them with the patient samples.
You did not answer this question.
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