HiTouch Flexi Plate-Aerobic Bacteria

As Touch Plate
For Sample Dilution
Your le-Free
Hass -effective
55 mm Scored Plates,
Also Available
Cost ce  Irradiated, Triple Pack

Ideal Choice of Food & Pharma
choi Also recommended for

water and envirnomental
testing
For Swabbing
HiTouch FlexiPlate
TM TM
Flexible Readymade Multifunctional Plates

HiMedia s handy, ready made touch plates For Surface monitoring
in Meat, Fisheries, Food & Beverage Industries, Dairies, Hospitals,
Biotechnology, Cell Culture, Pharmaceuticals & Cosmetics Environment
Multifunctional Readymade Mini Plates
HiMediaLaboratories TM
HiMedia Laboratories Pvt. Limited

www.himedialabs.com
HiTouch FlexiPlate
TM TM
Ready to use HiTouch Flexi Plates for Coliforms, Staphylococci,

Pseudomonas, Salmonella, Lactic acid bacteria, Yeasts and Moulds etc.
What are Flexi Plates ? Number of microorganisms Interpretation (1,2)
Hitouch flexiplates are ready to use sterile media supplied in flexible on surfaces in contact
disposable plates, 55 mm in diameter. It is grid scored on the base and is with food
irradiated to ensure perfect sterility. Medium is filled aseptically and each
plate is packed in pre-sterilized plastic bag. HiTouch Flexi Plate is then packed in
< 1/cm2 Excellent
plastic pouch wrapping. The unique flexible plate configuration ensures close 2 to 10/cm2 Good
contact even with uneven surfaces. These plates are specially developed for 11 to 100/cm2 Surface to be cleaned rapidly
microbial testing, where not only counts are obtained (as on FL001, FL009,
FL015, FL035, FL037) but it is also possible to select and differentiate between > 101/cm2 Stop the production
groups of microorganisms like coliforms (both E. coli and non E. coli), line and determine the
Staphylococci, lactic acid bacteria, Listeria, Salmonella, Ent. Sakazaki, yeasts and cause of contamination
moulds (FL002 - FL008, FL010, FL014, FL016, FL017, FL022-FL024, FL030-
FL034, FL036) and other microorganisms The Flexiplate medium formula is Examples of Interpretation according to the level of risk in
suitable for optimal growth of environmental microorganisms and the grids enable the zone to be tested :
direct reading on the plates of the number of colonies per cm2.  irradiated Flexi
Plate with neutralizers FL035G, FL039GT, FL041G & Flexi plate containing media
Level of Risk No. of colonies No. of colonies per
with Lactamases as FL038 recommended for pharmaceutical use also fall in this per 25 cm2(*) 100 cm2(**)
range. Moreover the neutralizing agents in FL009, FL015 & FL035 inactivate any 4 <5 < 10
residual disinfectants (3,4,5) present on the surface of the medium to be tested :
3 <5 < 100
The association between lecithin and polysorbate 80 neutralizes quaternary
ammonium compounds.
2 < 50 < 1000
Lecithin neutralizes chlorhexidine. 1 < 125 < 1000
Polysorbate 80 neutralizes hexachlorophene and mercurial compounds. Key : * = “Guide de bionettoyage.” Journal Official de la Republique Francaise.
Recommandation NOE 1-90 (1991) (6)
Where are Flexi plates used ? ** = Draft European Standard prEN 1632-3 (1993).
HiTouch Flexi plates are recommended to check critical control points in protected
areas while establishing or revising microbiological surface monitoring Limits of the Method :
techniques and programmes in hospitals, pharma industry, food & water testing The level of microorganisms recovery varies according to the characteristics of
and other public services. Flexi Plates can be used to control air, notably using an the surfaces and its adhesive properties (rough or smooth surfaces). HiTouch
atmospheric sample collector. Flexi Plate is a qualitative technique for the monitoring of surface
biocontamination. Taking into account the inherent inaccuracy of biological
How to use ? collections, it is important to use the same method of collection for all samples.
1. Open the Aluminium pouch in the airlock entrance chamber of the protected
References
area.
1. Hall L., Hartnett M.J., Pub. (1964) Health Rep., 79, 1021.
2. Remove the inner wrapping once in the protected area and read the instructions 2. Pryor A.K., Mc Duff C.R., Exed Housekeeper. (1969) Mass.
as specified on label before use. Samples must be collected on dry surfaces. 3. Lenette E.H., Spauldine E.H., Truant J.P. Manual of Clinical Microbiology,
2nd ed., (1974) American Society for Microbiology.
3. Apply the agar directly onto the surface to be tested ensuring that an even 4. Desbordes J., Biodegradation microbienne des antiseptiques et conservateurs.
pressure of 25 gm/cm2 is distributed over the whole plate for 10 seconds. (1977) Rev. Institut Pasteur de Lyon, 10 (nO4), 291-311.
4. Replace the lid. 5. Norme francaise NF T 72-151, Antiseptiques et desinfectants utilises a retat liquide,
miscibles a reau (03/1981).
5. Clean the surface where the sample was collected in order to remove any 6. “Guide du blonettoyage”. Journal Officiel de la Republique Francaise.
possible traces of agar. Recommandation NOE 1-90 (1991).
6. Incubate as recommended and enumerate the number of colonies. The mean
surface area of the plate is 25 cm2.
Interpretation of Results
Interpretations can vary from one laboratory to another, and each laboratory must
establish its own monitoring programme.
Classification of risk zones
Very low level of risk : 1 e iqu
e
iqu hn iqu
e
hn tec
Medium level of risk : 2 uch
tec
tive
po
ur
tati
ve
tec
hn
c e to ta ti
anti uan
High level of risk : 3 Su
rfa
Qu Q
Very high level of risk : 4
late
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asin tion e Fle tion
late ash b late 0-6 dilu salin unt -6 dilu ne
xiP xiP in Co . 10 in sali
Fle a in w Fle ue. 1 0nm rm e
u n t r e n t iq 2 lifo iqu 0nm
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ero app r o col tiv O.D
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h A uan
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ouc chniq : 75 ouc h q ted
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iT
H ch te ies HiT oug dju HiT throug adjus 7
01 u n 01 d thr ure a 18 0 2 d e 1
FL0 face to f colo FL0 culate d cult nies : FL0 culate cultur nies :
Sur ber o e Ino mixe f colo hn
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Ino . coli f colo
Num tec
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m
ing Num ce
tou N u
abb rfa
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u
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xiP
t P io M d late unt
As C ont ac Dilut ab ho xiP Co
e w et unt
Fle
sted for
m
For Sampl e d S gM ic Co ue adju Co
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li
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Fo r Mois te Settl ero E. ue ap
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n o .
ab s. E line ou hn
G r a vi ta t i o HiT sw a HiT ch tec basin 3
01 d by lonie in s 02 u sh 3
FL0 culate ble co 20nm FL0 face to ar wa nies :
Ino umera . at 6 Sur rea ne f colo
Inn .1 O.D to a ber o
to 0 Num
HiTouch FlexiPlate
TM TM

Recommended Test Procedures for Detection & Enumeration of Microorganisms*
using HiTouch™ Flexi Plates™
H iMedia's HiTouch™ FlexiPlates™ are specially developed for the microbial testing in food,
hospitals, water works, environmental testing etc.
Contact method
pharmaceutical, cosmetic, dairy,
Pressing Gently for examination Placing Medium back in Moulded Base Clossing and incubating
These plates are handy, ready to use, requires no preparation of media Alternative Methods of Inoculation :
and helps to obtain exact number of microorganisms. These plates are To use as Culture Plate (ii), Sample Dilution Plate (iii) or
very useful in detecting the presence of microorganisms by touching Swabbing Plate (iv)
surfaces as well as by conventional inoculation methods as described
1. HiTouch FlexiPlates can be used for determination of microbial level in
below.
liquids and fluids by dropping of 0.01 ml of sample dilution onto the
HiTouch Flexi Plates are securely fitted in a moulded base plate
medium surface.
with an attached lid to maintain the sterility of the plate. The diameter of
the prepared medium plate is 55 mm and the quantity of medium is 9 ml.
The plates are scored to facilitate counting of the colonies.
Advantages :
· No requirement of media preparation, sterilization etc.
· Saves time and labor
· Easy for enumeration , determines exact count of microorganisms
· Handy to use
· Occupies less storage space
· Direct enumeration from sample or surfaces
· No additional requirements of reagents
Sample dilution method
Multifunctional purposes :
1. Contact plates/touch plates 2. It is also possible to inoculate these plates by moistened swab after
2. Culture plates wiping off the sample area. In case of surface with heavy
3. Sample dilution plate contamination / surfaces not suitable for contact plates, the swab-
4. Swabbing plate rinse method may be used, where the moistened swab after stroking
5. Gravitational settling plate the sample area is dipped in sterile saline and agitated. A specified
How to use as Touch Plates by contact method (i) amount i.e 0.01 ml of this rinse fluid or its dilution is dropped on the
Open the lid and carefully lift up the enclosed prepared medium plate so as surface of flexiplates.
to avoid touching the agar surface by hand. Touch the surface of agar plate
onto the surface to be tested. Gently press the plate manually for upto 10
seconds. Apply constant and uniform pressure to the whole surface. Replace
exposed medium plate back in the base plate. Close the lid. Press the sides of
the lid to make sure that it is fixed in the grooves. Disinfect the surface where
the sample was taken in order to remove any possible traces of agar. Incubate
the plates at specified temperature. After incubation as recommended count
the number of colonies which have appeared on the surface of medium.
Recommended counting range for the flexiplates is 10-100 (CFU).The
number of colonies from each sample is expressed in colony forming units
(CFU) extrapolated to 100 cm2 of the surface sampled. According to the EU
GMP Guide (1997) the values for microbiological monitoring in the
operational state, for the manufacture of sterile product, the recommended
limits are as follows :
55 mm diameter scored Touch Plate Moistened swab method

Grade CFU / Plate
A <1 To use as Gravitation Settling Plate (v)
B 5 The flexiplates are also suitable for determination of airborne
C 25 microorganisms in room air and other environments by gravitation settling
D 50 of viable particles.
*(Ref. ISO14698-1 & 2)
1
HiTouch FlexiPlate
TM TM
FL009 HiTouch Enviromental Testing Flexi Plate

Lift up
Product details,
Name, Code
& Lot No.
Sterile medium
securely fitted in
moulded Base
Lift up
Flexi Plate medium enclosed View of Flexi plate medium

in moulded cover when opened
Illustrative Diagram for recommended use of HiTouch™ Flexi Plates™
Lid Top
Medium plate
moulded
container
Hold flexiplate moulded Carefully open lid

container as shown
S Top
Lid use as
Top touch plate
Medium
7
plate
Lift the
Close firmly and medium plate
Micropipette
incubate Swab
0
at 35-37 C or inoculate by
either method
To p
Testing
Procedure 4
pressing
6 Lift and put back
action
Place inverted on
into base plate test surface
The Touch Plate method would

5 follow the cycle : 1 - 2 - 3 - 4 - 5 - 6 - 7
Gently press manually
for 10 seconds
Flexi Plate used as Culture Plate, Sample Dilution Plate, Swabbing
Plate or Gravitation Settling Plate
would follow this cycle : 1 - 2 - S - 7
2
HiTouch FlexiPlate
TM TM
HiTouch™ Aerobic Count Flexi Plate™ FL001

For enumeration of aerobic microorganisms
Medium used : HiTouch™ Aerobic Count Medium Quality Control :

Composition** Appearance :
Ingredients Grams/Litre Sterile plastic plate containing light yellow coloured clear to slightly
opalescent firm gel.
Tryptose 15.00
Quantity of Medium :
Soypeptone 5.00 9 ml of gel in plastic plate.
Sodium chloride 5.00
Reaction :
Indicator dye 0.10 Reaction of the medium is pH 7.3 ± 0.2.
Agar 15.00 Sterility Test :
Final pH (at 25°C) 7.3 ± 0.2 Sterility of the medium was checked for 14 days. Medium was found sterile
** Formula adjusted, standardized to suit performance parameters without any colony formation on surface of solid medium.
Cultural Response :
User Profile :
Cultural characteristics observed after an incubation at 35-37°C for
Food & Dairy industries – raw materials, air & surface testing 18-24 hours.
Poultry & Meat – raw materials, air & surface testing Organisms (ATCC) Growth Colour of Colony
Pharmaceutical & – raw materials, air & surface testing
B. subtilis (6633) luxuriant red to maroon
Cosmetic industries
E. coli (25922) luxuriant red to maroon
Water & waste water – waste water E. faecalis (29212) luxuriant red to maroon
testing laboratories S. aureus (25923) luxuriant red to maroon
S. pyogenes (19615) luxuriant red to maroon
Hospitals, operation – for air & surface testing
theatres / I.C.U
Other industries, laboratories where microbiological
work is carried out
Salient features :
For detection and enumeration of aerobic microorganisms
Red coloured colonies due to presence of an indicator dye
Incubation temperature is 35 - 37oC
Quick results within 18 - 24 hours
Flexi Plate photograph in actual size
Storage
On receipt store between 2-8oC.
Shelf life 1 year.
Escherichia coli
FL001
3
HiTouch FlexiPlate
TM TM
HiTouch™ E. coli / Coliform Count Flexi Plate™ FL002

For enumeration of all coliforms along with differential count of E. coli
Medium used : HiTouch™ E.coli / Coliform Count Medium Quality Control :

Ingredients Grams/Litre Sterile plastic plate containg light yellow coloured clear to slightly opalescent
firm gel.
Tryptose 14.00
Peptone special 5.00
9 ml of gel in plastic plate.
Bile salts mixture 1.50
Reaction :
Disodium hydrogen phosphate 1.00 Reaction of the medium is pH 7.3 ± 0.2.
Monosodium dihydrogen phosphate 0.60 Sterility Test :
Sodium chloride 2.40 Sterility of the medium was checked for 14 days. Medium was found sterile
Chromogen 0.075 without any colony formation on surface of solid medium.
Indicator dye 0.10 Cultural Response :
Agar 12.00 Cultural characteristics observed after an incubation at 44°C for 18-24 hours.
Organisms (ATCC) Growth Colour of Colony
Final pH (at 25 C) 7.3 ± 0.2
0
E. coli (25922) luxuriant bluish purple

** Formula adjusted, standardized to suit performance parameters
K. pneumoniae (13883) luxuriant red to maroon
User Profile : S. aureus (25923) inhibited -
S. Enteritidis (13076) luxuriant red to maroon
Food and Dairy industries – raw materials, inprocess and finished
products
Pharmaceutical industry – raw materials and finished products
Meat & Poultry – raw materials and meat products
Water & waste water – waste water samples & recreational
testing laboratories water where coliforms & differential
count of E. coli is carried out
Vaccine & toxin – raw materials
manufacturing
Other laboratories where microbiological work is carried out
Salient features :
For enumeration of coliforms along with differential count of E. coli
Contains various selective agents, chromogenic compound
& indicator dye which makes the medium selective and differential
Coliforms ferment lactose, produce acid & gas, thus can be
differentiated from non-lactose fermentors
Covers all the important enteric bacteria like Salmonella, Shigella,
E. coli etc
Blue coloured colonies of E. coli & red coloured colonies of other
coliforms observed due to presence of an indicator dye
Incubation temperature is 44oC
Confirmed results within 18 - 24 hours
Count red colonies surrounded with yellow zone to get coliform count Salmonella Enteritidis
Storage
o
On receipt store between 2-8 C.
Shelf life 1 year. FL002
4
HiTouch FlexiPlate
TM TM
HiTouch™ Coliform Count Flexi Plate™ FL003

For enumeration of coliform bacteria
Medium used : HiTouch™ Coliform Count Medium Quality Control :

Ingredients Grams/Litre Sterile plastic plate containing reddish pink coloured clear to slightly
Peptone 7.00
Yeast extract 3.00
Lactose 10.00
Reaction :
Bile salts mixture 1.50 Reaction of the medium is pH 7.4 ± 0.2.
Sterility Test :
Indicator dye 0.03 Sterility of the medium was checked for 14 days. Medium was found sterile
Crystal violet 0.02 without any colony formation on surface of solid medium.
Agar 15.00 Cultural Response :
Final pH (at 25 C) 7.4 ± 0.2
0 Cultural characteristics observed after an incubation at 35-37°C
** Formula adjusted, standardized to suit performance parameters for 18 - 24 hours.
User Profile :
E. aerogenes (13048) luxuriant pink
Cosmetic & - raw materials E. coli (25922) luxuriant pinkish red with bile precipitate
Pharmaceutical industries S. aureus (25923) inhibited -
Food & dairy industries - raw materials, inprocess material S. Enteritidis (13076) luxuriant colourless
and finished products
Water & waste water - water samples for coliform detection
testing laboratories
Poultry & Meat industries - raw materials and meat products
Salient features :
For enumeration of enterobacters & coliforms
Contains bile nutrients along with a indicator dye which
makes medium more selective
E. coli forms red colonies with bile precipitation around it, which can be
differentiated from other red coloured colonies of coliforms
Differentiates coliforms from Enterobacters

Coliforms ferment lactose which produce red colonies
surrounded by red purple halo
Lactose non fermentors & late lactose fermentors produce pale colonies
Red coloured colonies with gas production give coliform count
Storage Escherichia coli

Shelf life 1 year.
FL003
5
HiTouch FlexiPlate
TM TM
HiTouch™ High Sensitivity Coliform Count Flexi Plate™ FL004

For enumeration of coliform bacteria
Medium used : HiTouch™ High Sensitivity Coliform Count Medium Quality Control :
Ingredients Grams/Litre Sterile plastic plate containing pink coloured clear to slightly opalescent firm
gel.
Biopeptone 5.00
Yeast extract 3.00
Lactose 10.00
Reaction :
D-Mannitol 5.00
Reaction of the medium is pH 7.3 ±0.2.
Sterility Test :
Sodium lauryl sulphate 0.20 Sterility of the medium was checked for 14 days. Medium was found sterile
Sodium deoxycholate 0.10 without any colony formation on surface of solid medium.
Agar 15.00 Cultural characteristics observed after an incubation at 41.5°C for
Final pH (at 250C) 7.3 ± 0.2 7-18 hours.
** Formula adjusted, standardized to suit performance parameters Organisms (ATCC) Growth Colour of Colony
E. coli (25922) luxuriant pink with bile precipitate
User Profile :
K. pneumoniae (13883) luxuriant pink
Water & waste water – water samples P. mirabilis (25933) luxuriant colourless
testing laboratories S. aureus (25923) inhibited -
Food & Dairy industries – Inprocess material Ent. faecalis (19433) inhibited -
Cosmetics & – raw materials and finished products
Pharmaceutical industries
Meat & Poultry – raw materials and meat products
Salient features :
For enumeration of faecal coliform bacteria
Coliforms give pink coloured colonies due to presence of an indicator dye
Medium contains selective agent which inhibits non-coliforms
E. coli shows pink coloured colonies with bile precipitate
around the colonies

Incubation temperature is 41.5oC
Results within 7 - 18 hours
Storage
On receipt store between 2-8oC
Shelf life 1 year.
Escherichia coli
FL004
6
HiTouch FlexiPlate
TM TM
HiTouch™ Rapid Coliform Count Flexi Plate™ FL005

For enumeration of the coliform bacteria
Medium used : HiTouch™ Rapid Coliform Count Medium Quality Control :

Ingredients Grams/Litre Sterile plastic plate containing deep pinkish purple coloured clear to slightly
Biopeptone 5.00 opalescent firm gel.
Yeast extract 3.00 Quantity of Medium :
Lactose 10.00
Reaction :
D-Mannitol 5.00
Reaction of the medium is pH 7.2 ± 0.2.
Sterility Testings :
Sodium lauryl sulphate 0.20 Sterility of the medium was checked for 14 days. Medium was found sterile
Sodium deoxycholate 0.10 without any colony formation on surface of solid medium.
Cultural characteristics an observed after incubation at 41.5°C
Agar 15.00
for 7-18 hours.
Final pH (at 25°C) 7.2 ± 0.2
E. coli (25922) luxuriant Yellow with yellow zones
User Profile : S. aureus (25923) inhibited -
Water & waste - water samples E. faecalis (29212) inhibited -
water testing laboratories
Food & Dairy industries - raw material, inprocess material and
finished product
Cosmetics & - raw material, inprocess material &
Pharmaceuticals industries finished product
Meat & Poultry - raw material & meat products
Salient features :
For enumeration of faecal coliform from non-faecal coliform
E. coli gives yellow coloured colonies with yellow zone
Medium contains selective agent which inhibits non-coliforms
Incubation temperature is 41.5oC

Storage
Shelf life 1 year. Escherichia coli
FL005
7
HiTouch FlexiPlate
TM TM
HiTouch™ Yeast and Mould Count Flexi Plate™ FL006

For enumeration of yeast & mould (fungal) population
Medium used : HiTouch™ Yeast and Mould Count Medium Quality Control :
Potato infusion 200.00
Dextrose 20.00 9 ml of gel in plastic plate.
Indicator dye 0.03
Reaction :
Agar 15.00 Reaction of the medium is pH 5.6 ± 0.2.
Final pH (at 250C) 5.6 ± 0.2 Sterility Testings :
** Formula adjusted, standardized to suit performance parameters Sterility of the medium was checked for 14 days. Medium was found sterile
without any colony formation on surface of solid medium.
User Profile :
Cultural Response :
Cosmetics & - for air & surface testing Cultural characteristics observed after an incubation at 22-28°C
Pharmaceutical industries for 48-72 hours.
Food industry - for the total aerobic count Organisms (ATCC) Growth Colour of Colony
from products & also for air &
C. albicans (10231) luxuriant pinkish white
surface testing S. cerevisiae (9763) luxuriant pinkish white
Water & waste water - for air & surface testing
Meat & Poultry - for the total aerobic count

from products & also for air &
surface testing
Hospital/Operation Theatre - for air & surface testing
Salient features :
For the enumeration of yeast and moulds from air & surface
Pink to red coloured colonies observed due
to the presence of an indicator dye
Medium promotes ascospore production

Acidic pH helps selective growth of yeast and moulds
Incubation temperature is 22-28oC
Storage
Shelf life 1 year.
Candida albicans
FL006
8
HiTouch FlexiPlate
TM TM
HiTouch™ Rapid S. aureus Count Flexi Plate™ FL007

For enumeration of S. aureus
Medium used : HiTouch™ Rapid S. aureus Count Medium Quality Control :

Sterile plastic plate containing red coloured clear to slightly opalescent firm
Ingredients Grams/Litre
gel.
Proteose peptone 10.00
Meat extract B 1.00 9 ml of gel in plastic plate.
Reaction :
D-Mannitol 10.00 Reaction of the medium is pH 7.4 ± 0.2.
Indicator dye 0.025 Sterility Testings :
Agar 15.00 Sterility of the medium was checked for 14 days. Medium was found sterile
Final pH (at 250C) 7.4 ± 0.2 without any colony formation on surface of solid medium.
** Formula adjusted, standardized to suit performance parameters Cultural Response :
Cultural characteristics observed after an incubation at 35 - 37°C
User Profile : for 18-48 hours.
Food & Dairy industries - raw material, inprocess material and Organisms (ATCC) Growth Colour of Colony
finished product
E. coli (25922) inhibited -
Meat & Poultry - raw material and meat products S. aureus (25923) good-luxuriant yellow
Cosmetic & - raw material & finished product S. epidermidis (12228) fair-good red
Water & waste water - recreational water samples
Hospital/Operation Theatre - surface and instruments testing
in hospitals
Salient features :
For enumeration of S. aureus
Detects specifically coagulase positive S. aureus
S. aureus are seen by yellow colonies with yellow zone
Rapid results within 18 - 48 hours
Storage
Shelf life 1 year.
Staphylococcus aureus
FL007
9
HiTouch FlexiPlate
TM TM
HiTouch™ Lactic Acid Count Flexi Plate™ FL008

For enumeration of lactic acid bacteria
Medium used : HiTouch™ Lactic Acid Count Medium Quality Control :

Ingredients Grams/Litre Sterile Plastic plate containing medium amber coloured clear to slightly
Meat extract B 10.00
Yeast extract 5.00
Reaction :
Dextrose 20.00
Polysorbate 80 1.00
Ammonium citrate 2.00 Sterility of the medium was checked for 14 days. Medium was found sterile
Sodium acetate 5.00 without any colony formation on surface of solid medium.
Inorganic salts 0.15 Cultural Response :
Dipotassium phosphate 2.00 Cultural characteristics observed after an incubation at 35-37°C
Agar 12.00 for 18-48 hours.
0
Final pH (at 25 C) 6.5 ± 0.2 Organisms (ATCC) Growth
** Formula adjusted, standardized to suit performance parameters L. acidophilus (19992) luxuriant
L. fermentum (9338) luxuriant
User Profile :
L. plantarum (8014) luxuriant
Food industry - inprocess material and finished product
Meat - raw material and meat products
Dairy industry - milk products & processed products
Salient features :
For easy enumeration of acid producing bacteria
Essential nutrients in this medium facilitates the growth of Lactobacilli
Incubate at 35 - 37oC
Storage
Shelf life 1 year.
Lactobacillus acidophilus
FL008
10
HiTouch FlexiPlate
TM TM
HiTouch™ Environmental Testing Flexi Plate™ FL009

For enumeration of environmental organisms
Medium used : HiTouch™ Environmental Testing Medium Quality Control :

Sterile Plastic plate containing yellow coloured clear to slightly opalescent firm
gel.
Yeast extract 2.00 9 ml of gel in plastic plate.
Reaction :
Lecithin 1.00 Reaction of the medium is pH 7.2 ± 0.2.
Polysorbate 80 7.00 Sterility Testings :
Dextrose 1.00 Sterility of the medium was checked for 14 days. Medium was found sterile
Sodium bisulphite 0.10 without any colony formation on surface of solid medium.

Agar 15.00 18-48 hours.
0
Final pH (at 25 C) 7.2 ± 0.2 Organisms (ATCC) Growth Colour of Colony
E. coli (25922) luxuriant pink to red
User Profile : S. aureus (25923) luxuriant pink to red
Food & Dairy industries - for environmental count
Cosmetic & - for environmental count
Meat & Poultry - for environmental count
Vaccine & Antibiotic - for environmental count
production
Other laboratories where environmental
count of microorganisms is essential
Salient features :
Contains essential nutrients which support growth of a number of organisms
Presence of lecithin & polysorbate 80 helps to recover microorganisms
from surfaces with residues of disinfectant
Pink to red coloured colonies appear because of indicator dye

Storage
Shelf life 1 year.
Escherichia coli
FL009
11
HiTouch FlexiPlate
TM TM
HiTouch™ Environmental Testing Flexi Plate™ ( irradiated) FL009G

For enumeration of environmental organisms
Medium used : HiTouch™ Environmental Testing Medium Quality Control :

Sterile Plastic plate containing yellow coloured clear to slightly opalescent firm
gel.
Reaction :
Dextrose 1.00 Sterility of the medium was checked for 14 days. Medium was found sterile
Sodium bisulphite 0.10 without any colony formation on surface of solid medium.

Agar 15.00 18-48 hours.
0
Final pH (at 25 C) 7.2 ± 0.2 Organisms (ATCC) Growth Colour of Colony
E. coli (25922) luxuriant pink to red
User Profile : S. aureus (25923) luxuriant pink to red
Food & Dairy industries - for environmental count
Cosmetic & - for environmental count
Meat & Poultry - for environmental count
Vaccine & Antibiotic - for environmental count
production
Other laboratories where environmental
count of microorganisms is essential
Salient features :
Contains essential nutrients which support growth of a number of organisms
Pink to red coloured colonies appear because of indicator dye

Storage
Shelf life 1 year.
Escherichia coli
FL009G
12
HiTouch FlexiPlate
TM TM
HiTouch™ Selective Yeast and Mould Count Flexi Plate™ FL010

For enumeration of yeasts & moulds
Medium used : HiTouch™ Selective Yeast and Mould Count Medium Quality Control :
Ingredients Grams/Litre Sterile plastic plate containing green coloured clear to slightly opalescent firm
gel.
Biopeptone 10.00
Yeast extract 9.00
Dextrose 50.00
Reaction :
Magnesium sulphate 2.10
Potassium phosphate 2.00
Diastase 0.05 Sterility of the medium was checked for 14 days. Medium was found sterile
Thiamine hydrochloride 0.05 without any colony formation on surface of solid medium.
Agar 15.00 Cultural characteristics was observed after incubation at 22 - 28°C
for 48 -72 hours.
Final pH (at 250C) 4.6 ± 0.2
C. albicans (10231) luxuriant greenish white
User Profile : S. cerevisiae (9763) good-luxuriant greenish white
Cosmetics & - raw materials, inprocess and finished
Pharmaceutical industries products
Veterinary & Vaccine - for environmental testing
Agriculture - raw material, soil samples
Food & Dairy industries - raw material, inprocess and processed
food products
Beverage industry - raw material and environmental testing
Salient features :
For enumeration of yeast and mould from environment
Contains essential nutrients and acidic pH which help
in the fast growth of yeast and mould

Storage
o
Shelf life 1 year.
Saccharomyces cerevisiae
FL010
13
HiTouch FlexiPlate
TM TM
HiTouch™ Flexi Plate™ - SB FL011

For enumeration of yeasts and moulds
Medium used : HiTouch™ Modified Sabouraud Count Medium Quality Control :

Sterile plastic plate containing light yellow coloured clear to slightly
Mycological peptone 10.00
Agar 15.00 Reaction :
Final pH (at 25 C) 5.6 ± 0.2
0
** Formula adjusted, standardized to suit performance parameters Sterility Testings :
Sterility of the medium was checked for 14 days. Medium was found sterile
User Profile :
Cosmetics industry - raw materials
Cultural Response :
Veterinary - products for veterinary inprocess Cultural characteristics observed after an incubation at 22 - 28°C.
testing material for 40-48 hours.
Water & waste water - recreational water Organisms (ATCC) Growth Colour of Colony
testing laboratories C. albicans (10231) luxuriant white
S. cerevisiae (9763) luxuriant white
Food industry - food products and also E. coli (25922) inhibited -
for air & surface testing
Pharmaceutical industry - inprocess and finished products
Meat & Poultry - raw materials, meat products
Salient features :
For detection of aerobic pathogenic fungi which may produce
infective spores
Storage
Shelf life 1 year.
Candida albicans
FL011
14
HiTouch FlexiPlate
TM TM
HiTouch™ Flexi Plate™ - VJ FL012

For enumeration of Staphylococcus aureus
Medium used : HiTouch™ Selective S. aureus Count Medium Quality Control :

Ingredients Grams/Litre Sterile Plastic plate containing reddish pink coloured clear to slightly
Mannitol 10.00 9 ml of gel in plastic plate.
Dipotassium phosphate 5.00
Reaction :
Lithium chloride 5.00 Reaction of the medium is pH 7.2 ± 0.2.
Glycine 10.00 Sterility Testings :
Agar 16.00
Cultural Response :
Final pH (at 25 C) 7.2 ± 0.2
0
Cultural characteristics observed after an incubation at 35 - 37°C

for 18-24 hours.
User Profile : Organisms (ATCC) Growth Colour of Colony
Cosmetics Industry - raw materials E. coli (25922) inhibited -
S. aureus (25923) luxuriant black with yellow halo
Food industry - for specific S. aureus counts from
S. epidermidis (12228) fair transluscent to blackish
in process and finished products
Dairy industry - raw materials
Meat - raw materials/meat products
Poultry - raw material
Other industries where microbiological analysis is required
Salient features :
For enumeration of Staphylococci in heavily contaminated foods and
clinical specimens
Detects coagulase positive & mannitol positive colonies
of Staphylococcus aureus
High glycine content and Lithium chloride inhibits contaminants.
S. aureus growth appears as black colonies with yellow halo

Confirmed identification within 18-24 hours
Storage
o
Shelf life 1 year.
FL012
15
HiTouch FlexiPlate
TM TM
HiTouch™ FlexiPlate™ - VR FL013

For enumeration of Enterobacteriaceae
Medium used : HiTouch™ Enterobacteriaceae Count Medium Quality Control :

Sterile Plastic plate containing reddish pink coloured clear to slightly
Biopeptone 10.00
Lactose 10.00
Bile salts 1.50
Reaction :
Sodium chloride 5.00 Reaction of the medium is pH 7.4 ± 0.2.
Indicator mixture 0.032
Final pH (at 250C) 7.4 ± 0.2 without any colony formation on surface of solid medium.
User Profile : Cultural characteristics observed after an incubation at 35-37°C
for 18-24 hours.
Food industry - for Enterobacter enumeration, for
inprocess materials & processed foods Organisms (ATCC) Growth Colour of Colony
Veterinary - raw materials E. aerogenes (13048) luxuriant pink-red
E. coli (25922) luxuriant pink-red with bile precipitate
Water and waste water - for detection of Enterobacters in water
S. aureus (25923) inhibited -
testing laboratories and waste water
S. Enteritidis (13076) luxuriant colourless
Dairy industry - raw material
Other industries/laboratories where microbiological
work is carried out
Salient features :
For enumeration of Enterobacteriaceae in raw foods
The colonies will appear pink because of an indicator dye
Contains Bile salt mixture & crystal violet which enhances the growth of
gram-negative intestinal bacteria and inhibiting gram-positive microbes
Count the pink colonies to get coliform count
Covers all Enterobacteriaceae species
Incubation temperature is 35-370C
Storage
Shelf life 1 year.
Enterobacter aerogenes
FL013
16
HiTouch FlexiPlate
TM TM
HiTouch™ Flexi Plate™ - CT FL014

For enumeration of Pseudomonas aeruginosa
Medium used : HiTouch™ Pseudomonas aeruginosa Count Medium Quality Control :

Gelatin Peptone 20.00
Inorganic salts 11.40 Quantity of Medium :
Cetrimide 0.30
Reaction :
Agar 16.00
Final pH (at 250C) 7.2 ± 0.2
User Profile : without any colony formation on surface of solid medium.
Cosmetic industry - raw materials Cultural Response :
Cultural characteristics observed after an incubation at 35-37°C.
Vaccine manufacturing - raw materials
for 18-24 hours.
Pharmaceutical industry - raw materials and finished products
Organisms (ATCC) Growth
Other industries & Pathological laboratories where
Ps. aeruginosa (27853) luxuriant (Colourless to green)
microbiological work is carried out.
E. coli (25922) inhibited
Stenotrophomonas maltophila (13637) inhibited
Salient features :
S. aureus (25923) inhibited
For the enumeration of Pseudomonas aeruginosa
Pseudomonas aeruginosa will appear as pigmented blue, blue green or
non-pigmented colony
Confirmed results within 18-24 hours
Storage
Shelf life 1 year.
Pseudomonas aeruginosa
FL014
17
HiTouch FlexiPlate
TM TM
HiTouch™ FlexiPlate™ - DA FL015

For determing bactericidal activity of disinfectants
Medium used : HiTouch™ Selective Environment Count Medium Quality Control :

Sterile Plastic plate containing purple coloured clear to slightly
Peptone, special 7.50
Neutralising agents 9.50 Reaction :
Agar 15.00
Cultural Response :
Final pH (at 25 C) 7.6 ± 0.2
0
Cultural characteristics observed after an incubation at 35 -37°C

for 18 - 24 hours.
User Profile : Organisms (ATCC) Growth

B. subtilis (6633) luxuriant
Environment - water & waste water analysis
E. coli (25922) luxuriant
Sanitary testing - disinfectant & surface testing S. aureus (25923) luxuriant
Salient features :
To enumerate organism from environment and to neutralise
antiseptics & disinfectants
Bacterial growth observed as yellow colonies due to the presence
of an indicator dye
Gives total bacterial count of the samples analysed
Storage
Shelf life 1 year.
Escherichia coli
FL015
18
HiTouch FlexiPlate
TM TM
HiTouch™ Flexi Plate™ - Bacillus Counts FL016

For enumeration of Bacillus species
Medium used : HiTouch™ Selective Bacillus Count Medium Quality Control :

Ingredients Grams/Litre Sterile plastic plate containing light amber coloured clear to slightly opalescent
firm gel.
Biopeptone 6.50
Reaction :
Final pH (at 250C) 7.4 ± 0.2 Sterility Testings :
User Profile :
Cultural Response :
Dairy industry - finished products Cultural characteristics observed after an incubation at 35-37°C for
Poultry & Meat - raw materials 16-18 hours.
Food industry - raw materials & processed foods Organisms (ATCC) Growth
Pharmaceutical industry - inprocess material and B. cereus (10876) luxuriant
finished products B. subtilis (6633) luxuriant
Water and waste water - waste water samples, recreational water
Other labs/industries where microbiological work is carried out
Salient features :
For enumeration of Bacillus species from various samples
Presence of nutritious substrates enhances the growth of Bacillus
Storage
Shelf life 1 year.
Bacillus cereus
FL016
19
HiTouch FlexiPlate
TM TM
HiTouch™ FlexiPlate™ - Thermophilic Bacillus FL017

For selective enumeration of thermophilic Bacillus
Medium used : HiTouch™ Thermophilic Bacillus Count Medium Quality Control :

Sterile plastic plate containing purple coloured clear to slightly opalescent
firm gel.
Casein enzyme hydrolysate 10.00
Indicator dye 0.04 Reaction :
Final pH (at 25oC) 6.7 ± 0.2 Sterility Testings :
User Profile :
Cultural Response :
Food industry - raw materials & processed food Cultural characteristics observed after an incubation at 55°C for 48 hours.
Dairy industry - raw materials & processed dairy Organisms (ATCC) Growth Colour of Colony
products
B. brevis (8246) good-luxuriant yellow
Salient features : B. coagulans (8038) good-luxuriant yellow
B. stearothermophilus (7953) good-luxuriant yellow
Easy enumeration of aciduric & thermophilic spore-formers from raw
and processed food products
Easy detection of mesophilic spore-formers (aerobic) from dairy products
Thermotolerant acid producers appear as yellow colonies due to the
presence of an indicator dye
Incubation temperature is 55oC
Results within 48 hours
Storage
Shelf life 1 year.
Bacillus stearothermophilus
FL017
20
HiTouch FlexiPlate
TM TM
HiTouch™ ECC Count Flexi Plate™ FL022

For detection and enumeration of Escherichia coli and coliforms
Medium used : HiTouch™ Sensitivity Coliform Count Medium Quality Control :

Ingredients Grams/Litre Sterile plastic plate containing light pink coloured opalescent clear to slightly
Casein enzymic hydrolysate 3.30
Buffering agent 1.60
Reaction :
Sodium pyruvate 1.00
L-Tryptophan 1.00 Sterility of the medium was checked for 14 days. Medium was found sterile
Sorbitol 1.00 without any colony formation on surface of solid medium.
Tergitol 0.15 Cultural Response :
Chromogenic indicator 0.43 Cultural characteristics was observed after an incubation at 35-37°C
for 18-24 hours.
Agar 10.00
Organisms (ATCC) Growth Colour of Colony SalmonGal X-
Final pH (at 25°C) 6.8 ± 0.2 glucuronide
C. freundii (8090) good-luxuriant salmon to red (big) + -
** Formula adjusted, standardized to suit performance parameters E. aerogenes (13048) good-luxuriant salmon to red + -
E. coli(0157 : H7 ) good-luxuriant salmon to red + -
User Profile : (NCTC 12900)
E. coli (25922) good-luxuriant dark blue to violet + -
Food and Dairy Industries - raw material, air & surface testing Ent. faecalis (29212) inhibited - -
S. Enteritidis (13076) good colourless - -
Poultry & Meat - raw material, air & surface testing S. flexneri (12022) good colourless to - +
slightly pink
Water & waste water - water samples
Other laboratories where microbiological work is carried out.
Salient features :
For simultaneous detection of E. coli and other coliforms.
Rich in ingredients that help even the sublethally injured
coliforms to grow rapidly.
Contains Tergitol 7 which inhibits gram-positive as well as some
gram-negative bacteria other than coliforms.
Presence of chromogens helps to differentiate between different

coliforms on basis of their contrast colony colours.
Incubation temperature is 35-37°C.
Results within 18-24 hours.
Storage
On receipt store between 2-8°C.
Shelf life 1 year.
Citrobacter freundii
FL022
21
HiTouch FlexiPlate
TM TM
HiTouch™ Salmonella Count Flexi Plate™ FL023

For selective isolation and enumeration of Salmonella Typhi and other Salmonella species
Medium used : HiTouch™ Salmonella Count Medium Quality Control :

Ingredients Grams/Litre Sterile plastic plate containing red coloured clear to slightly
Yeast extract 3.00
L-Lysine 5.00
Carbohydrate source 18.50
Reaction :
Bile salts 2.50 Sterility Testings :
Sodium thiosulphate 6.80 Sterility of the medium was checked for 14 days. Medium was found sterile
Ferric ammonium citrate 0.80 without any colony formation on surface of solid medium.
Phenol red 0.08 Cultural Response :
Agar 15.00 Cultural characteristics observed after an incubation at 35-37°C for
18-24 hours.
Final pH (at 25°C) 7.4 ± 0.2
E. aerogenes (13048) fair yellow
User Profile:
E. coli (25922) fair yellow
Food and Dairy Industries - raw materials, inprocess P. mirabilis (25933) good-luxuriant yellow (black centres)
and finished products P. vulgaris (13315) good-luxuriant yellow (black centres)
Poultry & Meat - raw materials, and meat products S. aureus (25923) inhibited -
S. dysenteriae (13313) good-luxuriant red
Pharmaceutical Industry - raw materials and finished products
S. Enteritidis (13076) good-luxuriant red with black centres
Water & waste water - water samples S. Paratyphi A (9150) good-luxuriant red
testing laboratories S. Paratyphi B (8739) good-luxuriant red with black centers
Other laboratories where microbiological work is carried out. S. Typhi (6539) good-luxuriant red with black centres
S. Typhimurium (14028) good-luxuriant red with black centres
Salient features : S. sonnei (25931) fair-good red
Selective medium for the isolation and enumeration of Salmonella

Typhi and other Salmonella species.
Contains Deoxycholate as a selective agent which inhibits gram-positive
microorganisms.
Salmonella utilizes carbohydrate and decarboxylase lysine
changing the pH to alkaline.
Salmonella shows black centred colonies due to H2S production.
Other coliforms show yellow colonies.
Confirmed results within 18-24 hours.
Salmonella Enteritidis
Storage
Shelf life 1 year.
Escherichia coli FL023

22
HiTouch FlexiPlate
TM TM
HiTouch™ Listeria Count Flexi Plate™ FL024

For isolation and enumeration of Listeria species
Medium used : HiTouch™ Listeria Count Medium Quality Control :

Ingredients Grams/Litre Sterile plastic plate containing dark amber coloured clear to
slightly opalescent firm gel.
Biopeptone 24.00
Lithium chloride 15.00 9 ml gel in plastic plate
Reaction :
Esculin 1.00 Reaction of the medium is pH 7.0 ± 0.2.
Ammonium ferric citrate 0.50 Sterility Testings :
Final pH (at 25°C) 7.0 ± 0.2
Cultural characteristics observed after an incubation at 35-37°C
Water and waste water - Water samples Organisms (ATCC) Growth Esculin hydrolysis
testing laboratories B. subtilis (6633) inhibited -
Clinical laboratories - Clinical samples E. faecalis (29212) inhibited -
E. hirae (10541) inhibited -
L. monocytogenes (19111) luxuriant +
Salient features : L. monocytogenes (19112) luxuriant +
L. monocytogenes (19117) luxuriant +
For enumeration of Listeria species from clinical specimens, water
S. aureus (25923) good -
supples etc.
KEY : + = Black zone around colonies.
Contains Lithium chloride and antibiotics which inhibit gram-negative
and most gram-positive organisms.
Black colonies of Listeria monocytogenes which hydrolyzes esculin to
esculatin.
Incubation temperature 35-37°C.
Storage
Shelf life 1 year.
Listeria monocytogenes
FL024
23
HiTouch FlexiPlate
TM TM
HiTouch™ Enteric Pathogen Count Flexi Plate™ FL030

For selective isolation and differentiation of coliform organisms and other enteric pathogens
Medium used : HiTouch™ Pathogen Count Medium Quality Control :

Ingredients Grams/Litre Sterile Plastic plate containing red w/purplish tinge coloured clear to
Lactose 10.00 9 ml of gel in plastic plate
Bile salts 1.50
Reaction :
Indicator dyes 0.01 Sterility Testings :
Final pH (at 25°C) 7.1 ± 0.2
Cultural characteristics observed after an incubation at 35-37C
Food and Dairy Industries - raw materials, inprocess Organisms (ATCC) Growth Colour of Colony
and finished products E. aerogenes (13048) luxuriant pink to red
Poultry & Meat - raw materials and meat products E. coli (25922) luxuriant pink to red w/ bile ppt
E. faecalis (29212) fair to good pale pink to red
Cosmetic and - raw materials
P. vulgaris (13315) luxuriant colourless
pharmaceutical industries S. aureus (25923) inhibited -
Water & waste water - water samples S. Enteritidis (13076) luxuriant colourless
testing Laboratories S. Paratyphi A (9150) luxuriant colourless
S. Paratyphi B (8739) luxuriant colourless
Other laboratories where microbiological work is carried out. S. Typhi (6539) luxuriant colourless
S. flexneri (12022) fair to good colourless
Salient features :
For selective isolation and differentiation of coliforms and
other enteric pathogens.
Contains bile nutrients along with indicator dyes as selective agents.
Coliforms ferment lactose, produce acid and gas which can be
differentiated from non-lactose fermenters.
E. coli form pinkish red colony with bile precipitate around it which can be
differentiated from other pinkish red coloured colonies of coliforms.

Storage
Shelf life 1 year.
Escherichia coli
FL030
24
HiTouch FlexiPlate
TM TM
HiTouch™ HexaCrome Flexi Plate™

For differentiation of six pathogenic organisms- Escherichia coli, Klebsiella, FL031
Enterococcus, Proteus, Staphylococcus aureus and Pseudomonas
Medium used : HiTouch™ HexaCrome Count Medium Quality Control :

Ingredients Grams/Litre Sterile Plastic plate containing light amber coloured clear to
Meat extract B 6.00
Casein enzymc hydrolysate 4.00
Reaction :
Chromogenic indicator 12.45
Agar 15.00
** Formula adjusted, standardized to suit performance parameters without any colony formation on surface of solid medium.
User Profile : Cultural Response :
Cultural characteristics observed after an incubation at 35-37C
Food & Dairy industries – raw materials, air & surface testing
for 18-24 hours.
Poultry & Meat – raw materials, air & surface testing
Pharmaceutical & – raw materials, air & surface testing Organisms (ATCC) Growth Colour of Colony
Cosmetic industries E. coli (25922) luxuriant purple
Water testing laboratories – waste water E. faecalis (29212) luxuriant blue - green
K. pneumoniae (13883) luxuriant blue - green (mucoid)
Hospitals, operation – for air & surface testing
P. mirabilis (10975) luxuriant light brown
theaters / I.C.U.
Ps. aeruginosa (27853) luxuriant colourless (greenish pigment)
Other industries, laboratories where microbiological may be observed)
work is carried out S. aureus (25923) luxuriant golden yellow
Salient features :
For differentiation of six pathogens on the basis of their contrast
colony colours.
Contains rich sources of amino acids which support the
growth of wide variety of organisms.
Facilitates and expedites the identification of some gram-negative
bacteria and gram-positive bacteria on the basis of reactions of genus
or species specific enzymes with chromogenic substrates.
The indole production by E. coli can be confirmed using DMACA discs

(DD040) to distinguish between E. coli and Enterobacter colonies.
Proteus colonies can be confirmed by using TDA reagent (R035)
for Tryptophan deamination reaction.
Recommended for use in place of MacConkey Agar.
Confirmed results within 18-24 hours. Enterococcus faecalis
Storage
On receipt store between 2-8°C. Pseudomonas aeruginosa
Shelf life 1 year.
Escherichia coli
FL031
25
HiTouch FlexiPlate
TM TM
HiTouch™ OGYE Flexi Plate™ FL032

For isolation, enumeration and differentiation of yeasts and moulds
Medium used : HiTouch™ OGYE Medium Quality Control :

Ingredients Grams/Litre Sterile Plastic plate containing light amber coloured clear to
Yeast extract 4.00
Carbohydrate source 20.00
Chromogenic mixture 1.10
Reaction :
Inhibitory agent 0.10
Agar 12.00
User Profile : Cultural Response :
Cultural characteristics observed after an incubation at 22-28°C
Cosmetics & - raw materials, inprocess and finished
for 48-72 hours.
Pharmaceutical industries products
Veterinary & Vaccine - for environmental testing Organisms (ATCC) Growth Colour of Colony
Agriculture - raw material, soil samples *A. brasiliensis (16404) luxuriant light blue w/ black spores
C. albicans (10231) luxuriant green
Food & Dairy industries - raw material, inprocess and processed
E. coli (25922) inhibited -
food products
S. cerevisiae (19615) luxuriant colourless
Beverage industry - raw material and environmental testing
Other laboratories where microbiological work is carried out * formerly known as A. niger
Salient features :
Selective and differential medium for yeast and moulds.
Contains inhibitory agent that inhibits the growth of Lactobacilli
encountered in milk and milk products.
Contains chromogenic substrate for differentiation.
Storage
Shelf life 1 year.
FL032
26
HiTouch FlexiPlate
TM TM
HiTouch™ Flexi Plate™ M-TA FL033

For isolation and enumeration of coliforms
Medium used: HiTouch™ Modified Tergitol 7 Agar Quality Control :

Ingredients Grams/Litre Sterile Plastic plate containing green coloured clear to slightly
Peptic digest of animal tissue 10.0
Meat extract 5.00 9 ml of gel in plastic plate.
Lactose 20.0 Reaction :
Tergitol 0.10 Reaction of the medium is pH 7.2 ± 0.2.
Bromo thymol blue 0.05 Sterility Testings :
1% Triphenyl Tetrazolium chloride solution 2.5 ml
Final pH (at 25C) 7.2 ± 0.2 Cultural Response :
** Formula adjusted, standardized to suit performance parameters Cultural characteristics was observed after an incubation at 35 - 37°C
for 18 - 24 hours.
User Profile : Organisms (ATCC) Growth Colour of Colony
Water and Waste water - water sample E. aerogenes (13048) luxuriant reddish brown
testing laboratories E. coli (25922) luxuriant yellow with red centre
P. vulgaris (13315) good yellow with red centre
Food and Dairy industry - raw materials, inprocess material
Ps. aeruginosa (27853) good red with bluish zone
S. aureus (25923) inhibited -
Cosmetics and - raw materials, inprocess material
S. Typhimurium (14028) luxuriant red with bluish zone
Pharmaceuticals Industries and finished products
Meat and poultry - raw material and meat products
Other laboratories where microbiological work is carried out.
Salient features :
For detection and enumeration of coliforms.
E. coli gives yellow coloured colonies with yellow zone.
Triphenyl Tetrazolium chloride allows earlier recognition and identification E. coli
of Escherichia coli and Enterobacter aerogenes in water and food.
Incubate at 35 -37°C.
Results within 18 - 24 hours.
Storage
Shelf life 1 year.
Proteus vulgaris
E. aerogenes FL033
27
HiTouch FlexiPlate
TM TM
HiTouch™ Flexi Plate™ TJA FL034

For isolation and enumeration of Lactobacilli
Medium used : HiTouch™ Tomato Juice Agar Quality Control :

Ingredients Grams/Litre Sterile plastic plate containing medium amber coloured clear
to slightly opalescent firm gel.
Tomato juice (400 ml) 20.0
Peptonized milk 10.0
Reaction :
Final pH (at 25C) 6.1 ± 0.2 Sterility Testings :
User Profile : without any colony formation on surface of solid medium.
For industry - inprocess material and finished product. Cultural Response :
Meat raw material and meat products. Cultural characteristics was observed after an incubation at 35 - 37°C
for 40 - 48 hours.
Dairy industry milk products and processed products.
L. acidophilus (4356) luxuriant
Salient features : L. casei (9595) luxuriant
For easy enumeration of lactobacilli. L. leichmannii (4797) luxuriant
S. aureus (25923) luxuriant
Essential nutrients in this medium facilitates the growth of Lactobacilli.
Incubate at 35 -37°C.
Storage
Shelf life 1 year.
Lactobacillus casei
FL034
28
HiTouch FlexiPlate
TM TM
HiTouch™ SCDA w/ Polysorbate 80 & Lecithin Flexi Plate™

for determining the efficiency of sanitization of containers , equipment surfaces, water miscible cosmetics,etc.
It can also be used to enumerate the organisms from water insoluble products and fatty products
FL035
containing preservatives or antimicrobials
Medium used : HiTouch™ Soyabean Casein Digest Agar Quality Control :

w/ Polysorbate 80 and Lecithin
Appearance :
Composition** Sterile plastic plate containing light to medium amber coloured clear to
Soypeptone 5.00
Reaction:
Sodium chloride 5.00 Reaction of the medium is pH 7.3 ± 0.2
Lecithin 0.70 Sterility Testings:
Polysorbate 80 (Tween 80) 5.00 Sterility of the medium was checked for 14 days. Medium was found sterile
Agar 15.00 without any colony formation on surface of solid medium.
Final pH (at 25°C) 7.3 ± 0.2 Cultural response:

Cultural characteristics observed after incubation at 35-37°C for 18-24
** Formula adjusted, standardized to suit performance parameters hours.
User profile :
Organisms (ATCC) Growth Colour of colony
Environment and sanitary testing S. aureus (25923) luxuriant Yellow to gold
For surface examination of surfaces and for the detection and enumeration of E. coli (25922) luxuriant colourless
microorganisms present on surfaces of sanitary importance. Ps. aeruginosa (27853) luxuriant may have
green pigment
Salient Features :
For easy enumeration of microorganisms of sanitary importance.

form surfaces with residues of disinfectant.
Incubation temperature is 35 - 37°C.
Storage
Shelf life 1 year.
FL035
29
HiTouch FlexiPlate
TM TM
HiTouch™ SCDA w/ Polysorbate 80 & Lecithin Flexi Plate™ (  irradiated)

for determining the efficiency of sanitization of containers , equipment surfaces, water miscible cosmetics,etc.
It can also be used to enumerate the organisms from water insoluble products and fatty products
FL035G
containing preservatives or antimicrobials

Appearance :
Soypeptone 5.00
Reaction:

User profile :
Environment and sanitary testing S. aureus (25923) luxuriant Yellow to gold
For surface examination of surfaces and for the detection and enumeration of E. coli (25922) luxuriant colourless
microorganisms present on surfaces of sanitary importance. Ps. aeruginosa (27853) luxuriant (may have
green pigment)
Salient Features :
Gamma irradiated
Storage
Shelf life 1 year.
FL035G
30
HiTouch FlexiPlate
TM TM
HiTouch™ SCDA w/ Polysorbate 80 & Lecithin Flexi Plate™

(  irradiated) (Triple Pack) FL035GT
for determining the efficiency of sanitization of containers , equipment surfaces, water miscible cosmetics,etc. It can also be
used to enumerate the organisms from water insoluble products and fatty products containing preservatives or antimicrobials

Appearance :
Soypeptone 5.00
Reaction:

User profile :
Environment and sanitary testing E. coli (25922) luxuriant colourless
For surface examination of surfaces and for the detection and enumeration of Ps. aeruginosa (27853) luxuriant (may have
microorganisms present on surfaces of sanitary importance. green pigment)
S. aureus (25923) luxuriant Yellow to gold
Salient Features :
Gamma irradiated, Triple Packed

Storage
Shelf life 1 year.
FL035GT
31
HiTouch FlexiPlate
TM TM
HiTouch™ HiCrome Ent.sakazakii Agar Flexi Plate™

For enumeration (count) and differentiation of Enterobacter sakazakii (Cronobacter sakazakii) FL036
Medium used : HiTouch™ HiCrome Ent.sakazakii Agar Medium Quality Control:

Soypeptone 5.00
Reaction :
Sodium deoxycholate 0.50 Reaction of the medium is pH 7.3 ± 0.2
Sodium thiosulphate 1.00 Sterility Testings :
Chromogenic mixture 10.17 Sterility of the medium was checked for 14 days. Medium was found sterile
Final pH (at 25C) 7.3 ± 0.2 Cultural response :
Cultural characteristics observed after incubation at 35-37C for 18-24
hours.
User profile :
Food & Dairy industries - for specific identification of
E. coli (25922) good-luxuriant yellow
Ent.sakazakii
E. aerogenes (13048) good-luxuriant bluish
Water& waste water testing - for detection of Enterobacters E. faecalis (29212) inhibited -
laboratories in water. E. sakazakii (12868) good-luxuriant blue
Clinical samples-Urine & - for differentiation of S. aureus (25923) inhibited -
faeces testing Ent.sakazakii from other K. pneumoniae (13883) good-luxuriant yellow (mucoid)
Enterobacteriacaeae
Salient Features :
For easy enumeration,differentiation and interpretation of Ent.sakazakii.
from food, water, sewage, urine and faeces samples
Chromogenic substance differentiates between E. coli, E. aerogenes,
E. sakazakii and K. pneumoniae.
Incubate on 35-37C.
Results within 18-24 hours.
Storage
Shelf life of 3 months.
Enterobacter sakazakii
FL036
32
HiTouch FlexiPlate
TM TM
HiTouch™ Flexi Plate™ -R2A FL037

For enumeration (count) of treated potable water using longer incubation periods
Medium used : HiTouch™ R2A Agar Medium Quality Control:

Sterile plastic plate containing yellow coloured clear to slightly opalescent
firm gel.
Casein acid hydrolysate 0.50
Dextrose 0.50 Reaction:
Starch, soluble 0.50 Reaction of the medium is pH 7.2 ± 0.2
Dipotassium phosphate 0.30 Sterility Testings :

Magnesium sulphate 0.024 Sterility of the medium was checked for 14 days. Medium was found sterile
Sodium pyruvate 0.30
Agar 15.00 Cultural response:
Final pH (at 25C) 7.2 ± 0.2 Cultural characteristics observed after an incubation at 35-370C for
24-72 hours.
Organisms (ATCC) Growth Colour of
User Profile: Colony
Water testing laboratories- treated potable water Candida albicans (10231) good-luxuriant colourless
Escherichia coli (25922) good-luxuriant colourless
Salient Features : Escherichia coli (8739) good-luxuriant colourless
This medium is standard medium recommended Salmonella Enteritidis good-luxuriant colourless
for heterotrophic plate counts. (13076)
Salmonella Typhi (6539) good-luxuriant colourless
It contains nutritionally rich substrates supporting growth
Enterococcus faecalis (29212) good-luxuriant colourless
of heterotrophs.
Incubation temperature is 35-37C.
Results within 24-72 hours (incubate longer if necessary).
Storage
Shelf life of 1 year
Escherichia coli
FL037
33
HiTouch FlexiPlate
TM TM
HiTouch™ SCDA w/ Lactamase Flexi Plate™

A general purpose medium for cultivation of wide variety of organisms and for inactivation of FL038
 lactam antibiotics
Medium used : HiTouch™ Soyabean Casein Digest Agar with Lactam Quality Control:
Sterile Plastic plate containing light yellow coloured clear to slightly
opalescent firm gel firm gel
Tryptone 15.00
Soyapeptone 5.00 Quantity of Medium :
9 ml of gel in plastic plate
Sodium Chloride 5.00
Agar 15.00 Reaction:
 Lactam 330IU Reaction of the medium is pH 7.3 ± 0.2
Final pH (at 25°C ) 7.3 ± 0.2 Sterility Testings :

without any colony formation on surface of solid medium
User Profile:
Pharmaceutical industry - Environmental Monitoring during aseptic Cultural response:
production of lactam antibiotics Cultural Characteristics observed after an incubation at 35-370C for18-24
Other industries where microbiological work is carried out hours.
Salient Features : Organisms (ATCC) Growth
For enumeration of microorganisms which otherwise would get E.coli (25922) luxuriant
inhibited in routine agar medium Proteus mirabilis (25933) luxuriant
Gives bacterial count of the environment analysed Ent.faecalis (29212) luxuriant
Incubation temperature is 35-37°C S.aureus (25923) luxuriant
Confirmed results within 18-24 hours S.aureus (29213) luxuriant
Storage
Shelf life of 1 year
FL038
34
HiTouch FlexiPlate
TM TM
HiTouch™ Sabouraud Dextrose Agar w/ LTHTh Flexi Plate™ FL039

For determining the efficiency of sanitization of containers, equipment surfaces, water miscible cosmetics etc.
Medium used: HiTouch™ Sabouraud Dextrose Agar w/ LTHTh Quality Control:

Sterile Plastic plate containing light amber coloured clear to slightly
Mycological Peptone 10.00 Quantity of Medium :
Dextrose 40.00 9 ml of gel in plastic plate
Lecithin 0.70
Reaction:
Agar 15.00 Reaction of the medium is pH 5.6 ± 0.2
Sodium thiosulphate 0.5
Histidine 0.5 Sterility Testings :
Polysorbate 80 5.00 without any colony formation on surface of solid medium
Final pH (at 250C ) 5.6 ± 0.2 Cultural response:

Cultural Characteristics observed after an incubation at 22-25°C for
40-48 hours.
User Profile:
Environment and Sanitary testing Colony
For surface examination of surfaces and for the detection and enumeration Candida albicans (10231) luxuriant white
of microorganisms present on surfaces of sanitary importance. Saccharomyces cereviasiae (9763) luxuriant white
Salient Features : Aspergillus brasiliensis (16404) *Good- luxuriant -
For easy enumeration of fungi of sanitary importance Trichophyton rubrum ( 28191) **Good- luxuriant -
Presence of lecithin and polysorbate 80 helps to recover microorganism * incubated for 72 hours
from surfaces with residues of disinfectant ** incubated for 5-7 days
Evaluation of samples collected from areas before and after the treatment
with disinfectant evaluates cleaning procedures in environmental
sanitation.
Incubation temperature is 22-25°C
Results within 40-48 hours
Storage
On receipt store between 2-8°C
Shelf life 1 year
Candida albicans
FL039
35
HiTouch FlexiPlate
TM TM
HiTouch™ Sabouraud Dextrose Agar w/ LTHTh Flexi Plate™

 irradiated) (Triple Pack) FL039GT
For determining the efficiency of sanitization of containers, equipment surfaces, water miscible cosmetics etc.
Medium used: HiTouch™ Sabouraud Dextrose Agar w/ LTHTh Quality Control:

Mycological Peptone 10.00
Dextrose 40.00 Quantity of Medium :
Lecithin 0.7
Polysorbate 80 5.0 Reaction:
Histidine 0.5 Reaction of the medium is pH 5.6 ± 0.2
Sodium thiosulphate 0.5 Sterility Testings :

Final pH (at 25°C ) 5.6 ± 0.2
** Formula adjusted, standardized to suit performance parameters Cultural response:
User Profile: Cultural Characteristics observed after an incubation at 22-25°C for
40-48 hours.
Environment and Sanitary testing
For surface examination of surfaces and for the detection and enumeration of Colony
microorganisms present on surfaces of sanitary importance.
Candida albicans (10231) luxuriant white
Salient Features :
Saccharomyces cereviasiae (9763) luxuriant white
Triple pack, Gamma irradiated
Aspergillus brasiliensis (16404) *Good- luxuriant -
For easy enumeration of fungi of sanitary importance Trichophyton rubrum ( 28191) **Good- luxuriant -
Presence of lecithin and polysorbate 80 helps to recover microorganism * incubated for 72 hours
** incubated for 5-7 days
Evaluation of samples collected from areas before and after the treatment
with disinfectant evaluates cleaning procedures in environmental
sanitation.
Storage
Shelf life 1 year
Aspergillus brasiliensis
FL039GT
36
HiTouch FlexiPlate
TM TM
HiTouch™ Sabouraud Chloramphenicol Agar Flexi Plate™ (  irradiated)

For selective cultivation of yeasts and moulds
FL041G
Medium used: HiTouch™ Sabouraud Chloramphenical Agar Quality Control:

opalescent firm gel
Peptone 5.00 Quantity of Medium :
Dextrose 40.00
Chloramphenicol 0.05 Reaction:
Agar 15.00 Reaction of the medium is pH 5.6 ± 0.2
Final pH (at 25°C) 5.6 ± 0.2 Sterility Testings :

User Profile:
Pharmaceutical industries: Microbial limit Tests for fungi-Yeasts and molds Cultural response:
Veterinary & Vaccines : General Purpose Culture media Cultural Characteristics observed after an incubation at 22-25°C for
40-48 hours
Agriculture : General Purpose Culture media
Clinical : Non selective culture media for solid media, Fungi- Yeasts and molds
Salient Features : Candida albicans (10231) luxuriant
Escherichia coli (25922) inhibited
Gamma irradiated
Lactobacillus casei (9595) inhibited
For enumeration of fungi-yeasts and molds
Aspergillus brasiliensis (16404) luxuriant
Contains inhibitory agent that inhibits growth of Gram positive S.aureus and
Gram negative E.coli Sachharomyces cerevisiae (9763) luxuriant

Storage
Shelf life 1 year
FL041G
37
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GMP
CERTIFIED HiMedia Laboratories Pvt. Ltd.
TM
Manufacturer's and Exporters of World's Best Quality Dehydrated Culture Media

Pioneer in Animal free Culture Media (BSE and TSE Free)
World's Largest Range of Veg Peptone
1500 Animal Free Culture Media & Full Range of Vegtable Based Media bases such as
TM TM TM
HiVeg Peptones HiVeg Extracts HiVeg Peptone C Soya Peptone
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HiVegTM Infusions HiVegTM Special Infusion HiVegTM Special Peptone Proteose Peptone B, HiVegTM
3500 Dehydrated Culture Media & Full Range of Media Base ..............such as
Peptones Yeast Extract Casein Enzyme Hydrolysate / Tryptone Peptonized Milk Liver Hydrolysate
Meat Extract Casein Acid Hydrolysate Tryptose Proteose Peptones Liver Extract
Beef Extract Gelatin Peptone Meat Peptone Brain Heart Infusion
World biggest Range (70 Products) of Chromogenic Media ........... and many more
HiCromeTM UTI Agar HiCromeTM Candida Differential Agar HiCromeTM Coliform Agar, Modified
HiCromeTM MeResa Agar Base HiCrome ListeriaTM Agar Base HiCromeTM KPC Agar Base
HiCromeTM VRE Agar Base HiCromeTM Ent.sakazakii Agar / Modified HiCromeTM ESBL Agar Base
Full Range of Dehydrated Media for Plant, Animal & Insect Cell Culture
Ready Prepared Media for Microbiology, Animal, Plant & Insect Cell Culture
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We also offer full range of Laboratory Chemicals and Biochemicals

Above 4000 Chemicals of purified /ACS /AR grade
Lab Consumables
Acids - LR and AR grade
Solvents - Pure / AR / HPLC grade
,
Plate a
HiTouch FlexiPlate
TM TM
S c ored & Pharm

m d
55 m e of Foo
c
Choi
Ideal
For use in clean as well as unprotected areas
Page
HiTouch Aerobic Count Flexi Plate FL001 3 HiTouch ECC Count Flexi Plate FL022
Page
21
Page
HiTouch E. coli / Coliform Count FlexiPlate FL002 HiTouch Salmonella Count Flexi Plate
Page
4 FL023 22
Page
HiTouch Coliform Count FlexiPlate FL003 HiTouch Listeria Count Flexi Plate
Page
5 FL024 23
HiTouch High Sensitivity Coliform Page Page

Count Flexi Plate
FL004 6 HiTouch Enteric Pathogen Count Flexi Plate FL030 24
Page
HiTouch Rapid Coliform Count Flexi Plate FL005 HiTouch HexaCrome Flexi Plate
Page
7 FL031 25
Page
HiTouch Yeast and Mould Count Flexi Plate FL006 HiTouch OGYE Flexi Plate
Page
8 FL032 26
Page
HiTouch Rapid S. aureus Count FlexiPlate FL007 HiTouch Flexi Plate M-TA
Page
9 FL033 27
Page
HiTouch Lactic Acid Count Flexi Plate FL008 10 HiTouch Flexi Plate TJA FL034
Page
28
Page
HiTouch Environmental Testing Flexi Plate FL009 HiTouch SCDA w/Polysorbate 80 Page
11 and Lecithin
FL035 29
HiTouch Environmental Testing Flexi Plate Page

HiTouch SCDA w/ Polysorbate 80 & Page
FL009G 12 FL035G
( irradiated) Lecithin Flexi Plate (  irradiated) 30
HiTouch Selective Yeast and Mould Count

FL010
Page HiTouch SCDA w/ Polysorbate 80 & Lecithin Page
Flexi Plate 13 Flexi Plate (  irradiated, Triple Pack)
FL035GT 31
Page
HiTouch Flexi Plate - SB FL011 HiTouch HiCrome Ent.sakazakii Agar Page
14 Flexi Plate
FL036 32
Page
HiTouch FlexiPlate - VJ FL012 HiTouch Flexi Plate R2A
Page
15 FL037 33
Page
HiTouch FlexiPlate - VR FL013 16 HiTouch SCDA w/ Lactamase Flexi Plate FL038
Page
34
Literature Code : TL013_07/HiTouch FlexiPlate/0715

HiTouch Flexi Plate - CT FL014
Page
HiTouch Sabouraud Dextrose Agar Page
17 w/ LTHTh Flexi Plate
FL039 35
Page
HiTouch FlexiPlate - DA FL015 HiTouch Sabouraud Dextrose Agar w/ LTHTh FL039GT Page
18 36
Flexi Plate ( irradiated, Triple Pack )
Page HiTouch Sabouraud Chloramphenicol Agar Page

HiTouch Flexi Plate - Bacillus Counts FL016 19 FL041G 37
Flexi Plate ( irradiated)
Page
HiTouch FlexiPlate - Thermophilic Bacillus FL017 20
TM : Trade Mark Owned by HiMedia Laboratories Pvt. Limited Packaging : Pack of 50

All rights reserved  HiMedia Laboratories Pvt. Limited, 2015
plates of 55 mm dia, grid scored on base,
sterile packed in individual plastic bags.
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GMP
CERTIFIED
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Tel: 00-91-22-6147 1919 Fax: 6147 1920, 2500 5764 Email : info@himedialabs.com Web : www.himedialabs.com

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