ACTIVITY 5C

QUALITATIVE TEST FOR

CHOLESTEROL

GROUP 4
OBJECTIVES:
> To discuss the principles behind the
qualitative tests for cholesterol
> To explain the effects of the different reagents
used in the activities
> To discuss the properties of cholesterol that
makes it responsive to Salkowski and
Liebermann-Burchard Test.
SCHEMATIC
DIAGRAM
 Homogenize Resuspend
20 g of beef the residue
Filter
fat with 30 in 30 mL
mL acetone acetone

PREPARATION Evaporate to Redissolve

Filter dryness 25 mL in 5 mL
Twice of acetone warm ethyl
filtrate alcohol

Add 1 mL of
1% digitonin Set aside
in 90% for 3 hrs.
alcohol
 To 1 mL of the
Dissolve a small
chloroform
portion of the
solution, add 1
SALKOWSKI residue in 2 mL
mL conc.
TEST: chloroform
Sulfuric acid

Shake and
stand
 Add 10 drops of
acetic anhydride
to the remaining Shake
Liebermann- chloroform
Burchard Test: solution

Add 2 drops of Mix and allow

conc. Sulfuric to stand for 5
acid mins.
SALKOWSKI
TEST