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  • Ziehl-Neelsen Stain Flowchart

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    Bayu Kresna Wiratama
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    The document provides instructions for Ziehl-Neelsen staining. The staining process involves adding carbolfuchsin stain to slides and heating them until steaming. The slides are then washed and decolorized with acid alcohol until no color remains. Next, methylene blue counterstain is added before washing and drying the slides.

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    The document provides instructions for Ziehl-Neelsen staining. The staining process involves adding carbolfuchsin stain to slides and heating them until steaming. The slides are then washed and decolorized with acid alcohol until no color remains. Next, methylene blue counterstain is added before washing and drying the slides.

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    7 views1 page

    Ziehl-Neelsen Stain Flowchart

    Uploaded by

    Bayu Kresna Wiratama
    The document provides instructions for Ziehl-Neelsen staining. The staining process involves adding carbolfuchsin stain to slides and heating them until steaming. The slides are then washed and decolorized with acid alcohol until no color remains. Next, methylene blue counterstain is added before washing and drying the slides.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
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    ZIEHL-NEELSEN STAINING

    Put your slide at staining rack


    Add CARBOL FUCHSIN ( R1) into surface of slides, with a
    Bunsen burner, heat the smear gently until steaming 3 times
    (do not boilling) wait for 5 minutes

    Wash with running water

    Decolorize slide with ACID ALCOHOL (R2) until no more


    colour appear in the washing (15-30 second)

    Wash with running water

    Add METHYLENE BLUE (R3) into surface of slides, wait


    for 30-60 second

    Wash with running water

    Air dry your slide

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