MEETING W/ BON:

Take out genome (miniprep)

Miniprep out genome from e. Coli cell
Colony PCR:
Take out a colony and use it as a template
Primers will only amplify promoters
Run on gel
Knockout:
Recombineering
Order primers to put in placeholder
PCR
DNA w/ missing part is joined together
CRISPR:
Jewett
GFP:
Does not stay fluoresced
GFP degrades
Represillator
3 interlocking promoters & repressors
Turn on GFP expressions
Repression will turn off something else
Whole cycle
If cell duplicates, GFP can already be fluoresced if same environment
Lifecycle:
Will die; lifecycle of a cell
Cell-free de/rehydration:
Collin’s Lab @ MIT
Talk to Adam
Did a similar project
Cell-free
CRISPR