The document discusses plans to meet with Bon and conduct various experiments including miniprepping the genome from E. coli cells, performing colony PCR to amplify promoters, knocking out genes using recombination and primers, using CRISPR with help from Jewett, troubleshooting GFP that does not stay fluorescent possibly due to degradation or with a repressilator, how the lifecycle of a cell causes it to die, related work on cell-free de/rehydration from Collin's Lab at MIT, and talking to Adam about a similar project using cell-free CRISPR.
The document discusses plans to meet with Bon and conduct various experiments including miniprepping the genome from E. coli cells, performing colony PCR to amplify promoters, knocking out genes using recombination and primers, using CRISPR with help from Jewett, troubleshooting GFP that does not stay fluorescent possibly due to degradation or with a repressilator, how the lifecycle of a cell causes it to die, related work on cell-free de/rehydration from Collin's Lab at MIT, and talking to Adam about a similar project using cell-free CRISPR.
The document discusses plans to meet with Bon and conduct various experiments including miniprepping the genome from E. coli cells, performing colony PCR to amplify promoters, knocking out genes using recombination and primers, using CRISPR with help from Jewett, troubleshooting GFP that does not stay fluorescent possibly due to degradation or with a repressilator, how the lifecycle of a cell causes it to die, related work on cell-free de/rehydration from Collin's Lab at MIT, and talking to Adam about a similar project using cell-free CRISPR.
Miniprep out genome from e. Coli cell Colony PCR: Take out a colony and use it as a template Primers will only amplify promoters Run on gel Knockout: Recombineering Order primers to put in placeholder PCR DNA w/ missing part is joined together CRISPR: Jewett GFP: Does not stay fluoresced GFP degrades Represillator 3 interlocking promoters & repressors Turn on GFP expressions Repression will turn off something else Whole cycle If cell duplicates, GFP can already be fluoresced if same environment Lifecycle: Will die; lifecycle of a cell Cell-free de/rehydration: Collin’s Lab @ MIT Talk to Adam Did a similar project Cell-free CRISPR