Halu Oleo Pharm.Sci. Vol. 1 No.

ACTIVITY TEST OF HAIR GROWTH AND IRRITATION TEST HAIR TONIC

PREPARATION FORMULA FOR JACKFRUIT SEED ETHANOL EXTRACT (Artocarpus
heterophyllus)

Astried Amalia Amanat1 , Suryani, Hasnawati 3

1 Pharmacy Study Program Faculty of Pharmacy University of Halu Oleo Kendari Southeast Sulawesi
Jl. HEA. Mokodompit, 93132

astried.amalia.a@gmail.com

ABSTRACT
Jackfruit seeds (Artocarpus heterophyllus) contain alkaloids, polyphenols, flavonoids, and saponins which have
activity as hair growth. This study aims to determine the activity as hair growth and determine the irritation
potential of hair tonic preparations from jackfruit seed ethanol extract. The ethanol extract of jackfruit seeds was
obtained by soxletation using 96% ethanol. The extract obtained was then used as an active ingredient in hair tonic
preparations with a variation of extract concentration of 5%; 7.5%; and 10%. Additional ingredients used are
ethanol, propylene glycol, propyl paraben, methyl paraben, sodium metabisulfite, menthol, distilled water and
vanilla flavoring. The preparation was tested for activity as a stimulator of hair growth using the Tanaka method
(1980) which was treated with rabbits (Oryctolagus cuniculus). Based on the results of the activity test shows that all
hair tonic dosage formulas have stimulating activity on hair growth. Jackfruit seed extract concentration which has
the biggest activity is 10%. Then an irrita tion potential test is conducted which refers to BPOM (2014). The most
effec tive preparations for the hair growth were tested using rabbits (Oryctolagus cuniculus), then pay attention to
the degree of irritation. The results of the degree of irritation score performed on male white rabbits is 0. So it can
be said that the preparation of hair tonic ethanol extract of jackfruit seeds does not cause irritation and is safe to
use.

Keywords: ethanol extract, jackfruit seeds, hair tonic, hair growth stimulator, irritation.

INTRODUCTION Hair loss ( efluvium ) is the release of

Hair is a crown for everyone because hair from the scalp. Hair has a period of

hair functions in addition to providing growth, rest and release. About 100 strands

warmth, protection, hair as well as for a day occur in hair loss . Excessive hair loss
beauty and supporting appearance. Healthy will cause baldness. Hair needs to be

hair has thick, black, shiny, non-tangled and maintained, cared for and given nutrients to
does not fall out. But not everyone can have be healthy and beautiful (Wasitaatmadja,

healthy hair, because it can be influenced by 1997). Preparations that are widely used to
various factors including age, genetics, stress, overcome the growth of hair loss, intensify

certain skin diseases, side effects of drugs hair growth and stimulatehair growth which
and food consumed.The characteristics of is considered the most effective is hair

hair damage include dull / not shiny, matted tonic (Ministry of Health, 1985).
/ unruly, oily, split ends, easily broken hair, Various cosmetic products have been

and excessive hair loss (Diana, 2014; developed to overcome the problem of hair
Purnamasari, 2013). loss and hair loss, both from synthetic
materials and from natural

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 Halu Oleo Pharm.Sci. Vol. 1 No. 2

ingredients. Preparations on the market showed positive results containing saponin

usually come from synthetic materials, even compounds, tannins,
though many medicinal plants around us can terpenoids,alkaloids, flavonoids and
be used as hair tonics. Synthetic ingredients polyphenols. Flavonoids function as
to deal with hair loss, including Minoxidil, in antibacterial works to inhibit bacteria so that
its use have side effects in the form of they can accelerate hair growth. Alkaloids
erythema, headaches, vertigo, edema. In line are secondary metabolites that can increase
with this, the concept of living back to hair growth and enlarge the hair shaft
nature began to be in demand and because of the increased supply of food
supported by the abundance of natural substances. Saponins have the ability to form
wealth in Indonesia (Nurjanah, 2014; foams so that they can clean the scalp from
Kuncari, 2015; Rahmawati et al., 2009). We dirt and its properties as contaminants,
have been developed therapies using natural consequently an increase in peripheral blood
materials y ang generally has several circulation can accelerate hair
advantages, among others fairly cheap and growth. Polyphenols and tannins can bind
widely available raw materials in Indonesia and protect the proteins needed for hair
(Kristishanti et al., 2011). growth (Delphin et al., 2014; Akib et al.,
Plants that can be used to help hair 2016; Sitompul, 2002 ; Jubaidah et al.,
growth are jackfruit seeds ( Artocarpus 2018 ).
heterophyllus ). Jackfruit seeds are very This research was previously conducted
useful in hair growth and keeping hair by phytochemical screening of jackfruit
healthy ( Maurya, 2017; Babu, 2017; Tejpal seeds, after which a preliminary test of hair
and Parle, 2016). Jackfruit plants are annual
growth activities using extracts, formulation
shrub plants that are widely used by the
of preparations with variations in
people of Indonesia, both as vegetables and
as constituents of a dish. Utilization of concentrations of 5%, 7.5%, 10% and tested
jackfruit is still very limited so that people the physical stability of the preparation, then
only consume fresh fruit flesh, while the the next researcher took the initiative to test
seeds are underutilized and are usually
hair growth activities and see the irritation
thrown away without further
potential of the hair tonic preparations that
processing. Jackfruit seeds have many useful
ingredients, such as minerals (calcium and have been formulated before.
phosphorus) and vitamins (vitamin A, vitamin METHODS
B and vitamin C) (Nusa, et al 2014). The This type of research
content of vitamin A, vitamin B is known is Experimental research with the design
to stimulate hair growth and keep hair
of Posttest With Control Group Design . This
healthy and shiny. Vitamin C is useful for
producing collagen which provides hair research was conducted at the

structure (Tambunan et al., 2014; Nurjannah Halu Oleo University Faculty of Pharmacy
et al., 2014). Jackfruit seed extract also Research Laboratory . The implementation of
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 Halu Oleo Pharm.Sci. Vol. 1 No. 2

this research has been approved by the Halu ethanol extract using 3 rabbits were tested
Oleo University Ethics Committee of Medical by male rabbits.
Faculty through the publication of Ethical The making of formulas in this study
Clearance (EC). refers to the hair tonic formula by Lestari
The tools that will be used in the research, (2018). Other additives used were
research are latent stirring rods, 96% ethanol, propylene glycol, sodium
glassware, elektromantel (Stuart®, drop metabisulfite, propyl paraben, methyl
pipette, measuring pipette (MC ®), analytical paraben, menthol, vanillin and distilled
scales (Precisa®), water bath (Memmert®), water.
hair shaver (gillate) , scissors, calipers, ruler, Weighing the ingredients used and
scissors, rotary vacuum evaporator , series of marked according to the name of
devices soclet, spoite, marker, small brush. the material. Jackfruit seed extract is
The materials to be used in the study are dissolved in propylene glycol and stirred until
distilled water, aluminum foil (Klin Pak ® ), homogeneous (obtained by solution
jackfruit seeds ( Artocarpus heterophyllus ) A). Sodium Metabisulfite is dissolved in 5 mL
obtained from Koronua Village, Soblakoa of distilled water (obtained B solution) and
District, South Konawe Regency, Southeast mixed with a solution of A. Methyl paraben
Sulawesi Province, 96% ethanol, male white and propyl paraben dissolved in 5 mL
rabbit ( Oryctolagus cuniculus ), filter paper, ethanol, then added menthol and mixed into
cotton, gauze ( One Med ® ), 70% alcohol, solution A and B to obtain C. Propylene glycol
menthol, methyl paraben, sodium solution added little by little into solution C,
metabisulfite, plaster (Hansplast®), propylene stirred homogeneously and then added
glycol, vanillin, positive control ( Hair distilled water until the volume limit reached
Growt ® minoksidil 2%), clear masking tape, 100 mL and added vanillin flavoring to taste,
distilled water, black cardboard paper. and stirred until homogeneous then labeled.
The stages carried out in this study
were taking and preparing jackfruit seeds,
determination of jackfruit seed plants,
extracting jackfruit seeds, hair tonic dosage
formulations, hair growth activity test,
preparation of jackfruit seeds ethanol extract
using 3 rabbits and male irritation test
formula. preparations of jackfruit seed

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 Halu Oleo Pharm.Sci. Vol. 1 No. 2

Table 1. Hair Tonic preparation formula longest strands and then placed on black
is made in 100 ml . cardboard. To simplify measurements, rabbit
Composition Function FI (%)(b/v) FII (%)(b/v) FIII (%)(b/v) hair is placed on clear tape and then

Jackfruit Active 5 7,5 10

measured by length of the sorong (Aini,
seeds substance
Ethanol Solvent 30,00 30,00 30,00 2017). Data collected in the form of
Propylene Kosolven 15,00 15,00 15,00
glycol and quantitative data. Quantitative data was
humectants
Propyl Preservative 0,01 0,01 0,01 obtained from the results of hair length
paraben
Methyl Preservative 0,1 0,1 0,1 measurements of hair growth activity tests.
paraben
Sodium 0,1 0,1 0,1
Metabisulfite Antioxidants Results of hair length measurements were
Mentol Giver cold 0,30 0,30 0,30
sensation tested statistically using the ANOVA test
and help
penetration method, SPSS 22.
Vanilin Flavoring qs qs qs

Distilled Carrier ad 100 mL ad 100 mL ad 100 mL

After preparation the best
water
preparation for stimulating hair growth is
Information: Ad 100 mL = enough volume to be 100 mL with
continued by testing the irritation potential
the addition of distilled water

Next is testing of hair growth activity. of the preparation. 3 healthy rabbits

This was done to determine the effect of the weighing 2-3 kg are used. This test is carried

formula of hair tonic on the ethanol extract out using patch test method. Before the

of jackfruit seeds in helping hair growth in treatment, the rabbit had shaved his hair 1

male rabbits. The treatment area was divided box to the left and 1 box to the right about

into 6 regions consisting of normal controls 2.5 x 2.5 cm. The rabbit is left for 24 hours

(without administration), negative controls before use. After shaving is complete, then

(base of hair tonic preparations), positive proceed with matching hair tonic formula.

controls (Hair growth minoxidil®) and hair Before applying the preparation, the rabbit's

tonic preparation formula 5% jackfruit seed skin is gently cleaned with cotton soaked

extract concentration; 7.5% and 10%. Tests with distilled water, then the hair tonic is

were carried out in the morning and evening applied evenly to the rabbit's skin (Iryoto,

with a volume of 1 mL in each treatment 2010). The dose used is 0.5 mL for the

area. The first day of delivery is considered to material in the form of liquid applied to the

be the 0th day. Provision of preparations is rabbit's back, 1 area for hair tonic to be

carried out for 21 days. Observation of hair tested and 1 area for negative control (hair

length of each region was carried out on the tonic without the extract of jackfruit ethanol

7th, 14th and 21st days. Before being seeds). Then cover it with sterile gauze and

measured, the hair was pulled as many as 5 glued it together with plaster, this gluing

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 Halu Oleo Pharm.Sci. Vol. 1 No. 2
must be maximal so that the gauze is not
separated (BPOM, 2014).
Hair tonic treatment is carried out for
24 hours, after which the hair tonic attached
to the rabbit's skin is cleaned and removed
using cotton soaked with distilled water,
observe for any toxic symptoms that arise,
namely primary irritation in the form of
erythema and edema for 24, 48 and 72 hours
on the clock and the same time (BPOM,
2014).
RESULTS AND DISCUSSION
1. Sampling
Sampling of jackfruit seeds (artocarpus
heterophylus) in the village of koronua,
Soblakoa Subdistrict, Konawe Regency,
South East Sulawesi Province. The jackfruit
seed sample used is a type of ripe salak
jackfruit. Jackfruit seed samples obtained
are sorted wet to separate impurities or
other foreign materials. Washing using
running water aims to remove impurities
attached to jackfruit seeds, washed jackfruit
seeds cut into small pieces to facilitate the
drying process (Anggraini, 2017).
Jackfruit seed samples are dried in the sun
with black cloth covered. The purpose of
closing with black cloth during drying is to
avoid direct contact between the sample and
the sun so that damage to the components
contained in the sample can be reduced and
the water content can be reduced (Anggraini,
2017).
5
Black cloth also absorbs heat so that the
drying process by using black cloth will not
interfere with the drying process. Scouring is
done to change the size of the sample to be
smaller with a larger surface area so that it
can facilitate the socletation process
(Harbone, 1996).
2. Plant Determination
Plant determination aims to ascertain the
correctness of jackfruit seeds used in the
study. The process of determination of
jackfruit seeds was carried out at the Teacher
Training and Education Faculty Biology
Laboratory, Halu Oleo Kendari University.
The results of the determination stated that
the samples used were true jackfruit seeds
with the species Artocarpus heterophyllus
Lamk.
3. Extraction
Extraction is the separation of one or
several materials from a solid or liquid with
the aid of a solvent. Soxhlet extraction
method is a method of extracting materials
in the form of solids with a continuous liquid
solvent. The equipment used is called Soxhlet
extractor (Depkes RI, 1985).
The 2,000 gram jackfruit seeds sample
was extracted using the socletation method
using 96% ethanol. A sample of 15 grams is
wrapped using filter paper and put in a
socklet tool. 96% ethanol solvent was put
into 500 ml soclet flask. In the process of
socletation, the solvent placed in the flask
 Halu Oleo Pharm.Sci. Vol. 1 No. 2
will evaporate when heated through the
soclet side pipe and cool down as it passes
through the condenser. The condensed
solvent will fall on the inside of the soclet
containing the sample wrapped in filter
paper and soak it to the top of the chiffon
tube. One socletation cycle can be said to
have passed, if the solvent-filled soclet tool
has been submerged to the top of the chiffon
tube, then all parts of the solvent will be
attracted and stored in the pumpkin where
the initial solvent is. Submerged dancer
solutions will penetrate the cell wall and
enter into cell cavities containing active
substances that will dissolve (Elda et al.,
2014).
The solvent used in this study was 96%
ethanol. The choice of ethanol as a solvent is
because ethanol is relatively less toxic than
methanol, cheap, easy to obtain and the
extract obtained is not easily overgrown with
fungi and bacteria and is commonly used in
making extracts. In addition, ethanol is
semipolar so that polar and non-polar
compounds found in simplicia can be
attracted.
The extract produced was evaporated
using a rotary vacuum evaporator at 50oC to
separate the solvent from the filtrate. then
concentrated using a water bath at 50 ° C.
The use of a water bath to vaporize ethanol
solvents so that they are separated from the
extract until a thick extract of 166.9 grams is
6
obtained. The thick extract of jackfruit seeds
obtained a yield of 8.345%. The results of
jackfruit seed extract are dark brown in black
with the distinctive aroma of jackfruit seeds.
4. Hair Tonic Formulations
The concentration of jackfruit seed
extract contained in the formula was
determined based on a preliminary test
conducted by Zainuddin (2018) which stated
that the optimal concentration of jackfruit
seed extract can help hair growth starting at
concentrations of 5%, 7.5% and 10%. Based
on this description, researchers were
interested in developing the use of jackfruit
seeds by making jackfruit seed extract
formulations into hair tonic preparations.
The type of hair care cosmetics that are
effective for hair loss is hair tonic. Hair tonic
dosage form in the form of a solution that is
easy to apply and convenient for hair skin,
easy to spread, not sticky, and does not leave
a residue that can trigger dandruff (Aini,
2017). Additional ingredients used are
ethanol, propylene glycol, menthol, sodium
metabisulfite, propyl paraben, methyl
paraben, vanillin and aquades.
Ethanol is used as a solvent, propylene
glycol is mainly used to increase the solubility
of the ingredients contained in formulations
and as a humectant because of its nature
that can withstand water absorption. Ethanol
has helped the solubility of the thick extract
used but not completely dissolved. Therefore
 Halu Oleo Pharm.Sci. Vol. 1 No. 2
propylene glycol is also used to further
increase the solubility of thick extracts to be
completely dissolved. In addition, propylene
glycol also helps control the viscosity of the
preparation by maintaining a fixed solution
on the scalp so that contact with the scalp is
longer and preparations penetrate more
optimally (Rowe et al., 2009).
Menthol is used as a penetration
enhancer for the skin, the mechanism of
action is to improve the permeability of the
membrane so that the active substance
penetrates well on the skin. Besides being
used to provide a cold sensation on the
scalp, Menthol is also used to provide a fresh
odor and can increase penetration to the
skin (Rowe et al., 2009).
Sodium metabisulfite is used as an
antioxidant in water-based preparations to
prevent oxidation processes that may occur
in the preparation. Methyl paraben and
propyl paraben and used as preservatives, a
preservative combination is carried out
because of the amount of water that is large
enough in hair tonic which can be a medium
for microbial growth, so that the
combination of these two preservatives can
expand the spectrum activity of these
preservatives. Vanillin is used as a flavoring
and to increase the aesthetics of the
preparation (Rowe et al., 2009).
7
5. Hair Growth Activity Test
This test was conducted to determine
the activity of hair tonic preparations of
ethanol extract of jackfruit seeds (Artocarpus
heterophyllus) in male rabbits. The
treatment group was divided into normal
control groups (without administration),
positive controls (circulating synthetic
preparations), negative controls (bases) and
hair tonic formulas with extract
concentrations of 5%, 7.5% and 10%. The
concentration was chosen based on a
preliminary test of the jackfruit seed ethanol
extract conducted by Zainudin (2018). The
concentration of 5%, 7.5 and 10% is what has
activity as hair growth. The measurement of
hair length is carried out for 21 days. On the
7th day (1st week) hair growth can be seen
by the eye so that measurements can be
made. Subsequent measurements were
carried out at the 2nd and 3rd week. From
the hair length measurement data obtained
then the average hair length of each
treatment was calculated from 3 male
rabbits. After testing the test animals rabbits
are treated and fed and drunk. The test
animal is not turned off because testing is
only on the body surface, does not affect the
systemic and organs. So that it does not
harm humans and the environment. The
results of measurements of rabbit hair length
can be seen in Table 6.
 Halu Oleo Pharm.Sci. Vol. 1 No. 2

Table 6. Average length of rabbit hair during based on the significance level of each
treatment. treatment area (P 5 0.05), then continued on
Average Treatment of Hair Length (mm) ± SD homogeneity tests to see homogeneous
1st Week 2nd Week 3rd Week data populations with significant provisions
Normal (P 5 0.05) which means the data has the
control 2.51 ± 0.36 4.38 ± 0.35 5.48 ± 0.46
Positive same or homogeneous nature.
Control 6.7 ± 0.49 7.59 ± 0.59 9.66 ± 0.51
The homogeneity test results for week
Negative
Control 2.7 ± 0.32 4.52 ± 0.37 5.6 ± 0.43 1, week 2 and week 3, showed homogeneous

Formula I 8.9 ± 1.11 10.39 ± 1.45 12.36 ± 0.88 data assessed from (P 5 0.05), so that it could
be continued with ANOVA test. showed that
Formula II 11.54 ± 1.01 13.54 ± 0.84 15.93 ± 0.46
there were significant differences
Formula III 14.41 ± 0.5 16.92 ± 0.83 19.31 ± 0.59
(significantly different) between week 1,
Observation of rabbit hair growth for 3
week 2 and week 3 data of hair length
weeks revealed that all treatment groups
between treatment areas. Rabbit hair length
experienced growth in hair length as seen in
data between treatment areas have
the rabbit hair length graph which
significant differences (significantly different)
experienced an increase every week (Figure
so that it can be said that all hair tonic
17).
formulas have activities to stimulate hair
growth.
25
The test was continued with the Least
Rabbit hair length

20 Kontrol normal
15 Kontrol positif Significant Differences (LSD) test, aimed at
(mm)

10 Kontrol negatif
5 analyzing data groups that had significant
Formula A (5%)
0 differences and groups of data that had no
Formula B (7,5%)
1 2 3
Time (weeks) Formula C (10%) significant differences. Data on rabbit hair
length at week 1, week 2 and week 3 on the
Data obtained from the results of test of
line of treatment of positive control, formula
hair growth activity in rabbits were analyzed
1, formula 2, formula 3 has a significant
using ANOVA test to determine whether the
difference in significant values not greater
average rabbit hair length of each treatment
than 0.05 in the LSD test table ( Appendix
was different or not. Data ANOVA
10). This shows the hair growth of each
requirements must be normally distributed.
treatment is different because the
The results of the normality analysis showed
concentration of extract increases so that the
that rabbit hair length in each treatment
effectiveness of active substances has the
area was normally distributed, which was

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 Halu Oleo Pharm.Sci. Vol. 1 No. 2
ability to stimulate hair growth getting better
and triggered by the addition of hair tonic
formula to help trigger hair growth. In the
normal control line LSD test table it can be
seen that the negative control (preparation
basis) does not have a significant difference
(not significantly different) can be seen in the
LSD test. This shows that additional
ingredients do not play an active role in
providing hair growth activities.
The chemical content of active
substances that are efficacious as hair
growers also increases so that the
effectiveness of growth. Hair tonic jackfruit
seed extract contains secondary metabolites
of alkaloids, flavonoids, saponins,
polyphenols, tannins and vitamins and
nutrients that can stimulate hair growth
(Zainudin, 2018).
Alkaloids are secondary metabolites that
can increase hair growth and enlarge the hair
shaft because the supply of food substances
increases (Jubaidah et al., 2018).
Flavonoids as a group of many phenolic
compounds found in plant tissues can act as
antioxidants. Free radicals are one of the
causes of hair loss, so that flavonoids can
prevent free radicals and accelerate hair
growth (Sitompul, 2002).
Saponin has the ability to form foam
which means it is able to cleanse the skin
from dirt, besides it functions to increase
9
blood flow to the hair follicles (Nurjannah et
al., 2014).
Polyphenols and tannins function as
antioxidants to reduce free radicals and as
antibacterial and antiviral agents work to
inhibit bacteria and viruses so they can
accelerate growth and prevent hair loss
(Robinson, 1995). In addition, with the form
of hair tonic preparations have very good
absorption by the scalp and facilitate blood
circulation (Depkes RI, 1985).
Nutrition plays a role in stimulating hair
growth. Water is an important nutrient
because almost a quarter of the weight of
hair consists of water. Moisture due to the
presence of water causes the hair to become
soft. In addition to malnutrition, it affects
hair growth, especially protein and calorie
malnutrition, where 98% protein
consumption of foods with high protein
content can nourish hair, vitamin A obtained
through retinol obtained from foods derived
from animals and beta carotene obtained
from foods originating from plants, vitamin E
obtained from nuts, seeds, grains, avocados,
eggs, milk, meat, soybean oil, cabbage and
some vegetables such as spinach and other
broccoli, vitamin B complex, vitamin C,
iodine, substances iron, and cysteine,
(Soedibyo et al., 1999).
 Halu Oleo Pharm.Sci. Vol. 1 No. 2

6. Potential Irritation Test and 1 box to the right. After leaving it for 24
The test animals used in this study were hours to get used to the rabbit. Then apply
male white rabbits (Oryctolagus cuniculus), the preparation then cover with sterile
the selection of male white rabbits due to gauze. Observations for the irritation test
having sensitive skin than other types of were carried out at 1 hour, 24 hours, 48
rabbits and based on white rabbit hair more hours and 72 hours after the test preparation
easily shaved than other types of rabbits. The was given by observing the skin reactions
purpose of this study was to see whether the that arise with 2 observational parameters,
hair tonic preparation of jackfruit seeds namely the level of erythema (redness
ethanol extract is safe to use and to reaction) and the level of edema (swelling)
determine the level of erythema and edema that arises. Observations were made at 24,
and its primary irritant index (BPOM, 2014). 48 and 72 hours after the bandage was
In topical preparations, one of the released aiming to determine the possibility
important parameters to note is the of a delayed irritation reaction. Then the
possibility of the product being applied to results of these observations are given a
cause irritation to the skin. The appearance score of 0 to 4 according to the severity. The
of irritation can occur after a period of time test animal is not turned off because testing
from the application of the preparation, is only on the body surface, does not affect
characterized by several symptoms such as the systemic and organs. So that it does not
the skin will dry up feeling painful, bleeding, harm humans and the environment. The
and cracking. Irritations that occur in the skin level of irritation is calculated based on the
are characterized by erythema and edema, calculation of the observation score (Ditjen
where erythema or redness occurs due to POM, 2014).
dilation of blood vessels in an irritated area, Table 7. Irritation Test Results on Male White
whereas in edema there is enlarged plasma Rabbits
24 hours 48 hours 72 hours
No.
enlargement in the injured area (Pratimasari Eyi the Ede
rabbit Erythema Edema Erythema Edema
ma ma
et al., 2015). 1 0 0 0 0 0 0
2 0 0 0 0 0 0
The irritation test was carried out in this 3 0 0 0 0 0 0
Total 0 0 0 0 0 0
study, which was carried out on three Pri ma ry
Irri ta tion 0
animals. This test is done by applying a hair Index

tonic preparation and dosage base to the From the results of observations in the

back area of the test animal. The back of the table, for erythema that occurs in rabbits 1,

test animal was shaved 1 box before the left 2, and 3 at 24 hours a score of 0 is obtained
(without erythema) whereas for edema
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 Halu Oleo Pharm.Sci. Vol. 1 No. 2
scores of rabbits 1, 2 and 3 at 24 hours a
score of 0 is obtained because at the time of
observation no edema formation reaction
was seen. Likewise at the 48th and 72nd
hours for erythema and edema scores in
rabbits 1, 2 and 3 obtained a score of 0. With
the results of observations on rabbits 1, 2
and 3 at 24, 48 and 72 hours 0, then the
result of the degree of irritation score is 0.
This shows that the hair tonic preparation of
jackfruit seed extract applied to the skin of
the male rabbit does not cause undesirable
effects on the skin in this case it is irritation.
CONCLUSION
1. Formula for hair tonic preparations with a
concentration of 5%, 7.5% and 10% ethanol
extract of jackfruit seeds has hair growth
activity in rabbits, 10% concentration has the
best activity in accelerating rabbit hair
growth and can replace positive control (Hair
growth Minoksidil® )
2. The result of the degree of irritation score
in male white rabbits is 0, so it can be said
that the hair tonic preparations of the
ethanol extract of jackfruit seeds do not
cause irritating effects and are safe to use.
REFERENCES
Achmad A.S., 1990, Hakim E.H., dan Makmur
L., Flavonoid dan Fitomedika,
Kegunaan dan Prospek, Jakarta: Phyto-
Medika.
Azis, Sriana, dan S.R. Muktiningsih., 1999,
Artikel Studi Kegunaan Sediaan
Rambut, Jakarta: Puslitbang Farmasi
Badan Litbangkes.
11
Apriyani, Delta dan Marwiyah., 2014,
Pengaruh Nanas (Ananas Comosus)
Terhadap Rambut Berketombe
(Dandruff) Pada Mahasiswa Pendidikan
Tata Kecantikan, Journal of Beauty and
Beauty Health Education JBBHE, 3 (1).
Akib, Nur I., Salim, Armin N.A., Malaka, M.
H., Baka, W.A., 2016, Development
and Evaluation of Waru (Hibiscus
tiliaceus Linn.) Leaf and Avocado
(Persea americana Mill.) Fruit Extracts
for Hair Growth, International Journal
of Chemical, Environmental &
Biological Sciences (IJCEBS), 4(2).
Anggraini, S., Safitriani, R. R., dan Santosa,
U., 2007, Pengaruh Penutupan
Dengan Kain Hitam Dan Konsentrasi
Etanol Terhadap Kandungan
Kurkuminoid Dan Aktivitas
Antioksidan Ekstrak Simplisia
Temulawak (Curcuma xanthrrhiza),
Jurnal Teknologi dan Industri
Pangan, 18(2).
Arista, Mega., 2013, Aktivitas Antioksidan
Ekstrak etanol 80% dan 96% daun
katuk (Sauropus androgynus (L.)
Merr.) Jurnal Ilmiah Mahasiswa
Universitas Surabaya. 2(2).
Aini,Q., 2017, Uji Aktivitas Pertumbuhan
Rambut Kelinci Jantan Dari Sediaan
Hair Tonic Yang Mengandung Ekstrak
Etanol Daun Mangkokan (Nothopanax
scutellarium L.), Jurnal Farmasi
Lampung, 6 (2).
Balsam, M.S., and Sagarin, E., 1974,
Cosmetic Science and Technology,
Vol.III, 2nd Ed., Wiley Interscience, a
division of Wiley and Son, New York.
BPOM, 2014, Pedoman Uji Toksisitas
Nonklinik Secara In Vivo, Depkes RI,
Jakarta.
Babu, Nandhitha G., 2017. Extraction And
Comparison Of Properties Of Jackfruit
 Halu Oleo Pharm.Sci. Vol. 1 No. 2

Seed Oil And Sunflower Seed Oil, Ekstrak Daun Kembang Sepatu
International Journal of Scientific & (Hibiscus rosa-sinensis) Pada
Engineering Research, 8 (11). Pertumbuhan Rambut Kelinci. Jurnal
Farmasi Indonesia, 8(1), 262-263.
Departemen Kesehatan RI, 1985,
Formularium Kosmetika Indonesia, Heyne, K, 1987, Tumbuhan Berguna
Jakarta: Departemen Kesehatan RI. Indonesia Jilid 3, Departemen
Kehutanan, Jakarta.
Damron, M., 2003, Klasifikasi Makhluk Hidup
: Mamali, Gramedia Pustaka Utama, Harbone, J. B., 1996, Metode Fitokimia:
dan Keindahan Rambut: Jakarta. Penuntun Cara Modern
Menganalisa Tumbuhan (K.
Delphin.D.V., Haripriya.R., Subi.S., Jothi.D., Padmawinata, dan I. Soediro,
Thirumalai P.V., 2014, Phytochemical Penerjemah). Penerbit ITB, Bandung.
Screening Of Various Ethanolic Seed
Extracts, World Journal Of Pharmacy Hustamin, R, 2006, Panduan Memelihara
And Pharmaceutical Sciences, Vol. 3 Kelinci Hias, PT Agro Media Pustaka:
(7). Jakarta Selatan.

Diana, W, 2014, Penggunaan Ekstrak Buah Handayani, C.S., Indri, H., dan Susanti, 2009,
Alpukat Dan Madu Sebagai Bahan Aktif Uji Fototoksisitas Sediaan Krim Muka
Hair Tonic Untuk Rambut Rontok, e- “X” terhadap Kelinci Putih Jantan.
Journal, 3(1). Pharmacy, Vol. 6(1).

Darwis, D., 2000, Teknik Dasar Laboratorium Iryoto, G., Ika, Y.H dan Didik, S., 2010, Sifat
Dalam Penelitian Senyawa Bahan Alam Fisik dan Uji Iritasi Primer Gel Ekstrak
Hayati, Workshop Pengembangan Etanol Buah Mahkota Dewa
Sumber Daya Alam Hayati dan Phaleriamacrocarpa (Schft Boert)
Rekayasa Bioteknologi, FMIPA, dengan Basis CMC Na dan Aqupect
Universitas Andalas, Padang. 505, Pharmacy, 7(2).

Ditjen POM., 2000, Parameter Standar Illing, I., Wulan, S., dan Erfiana, 2017, Uji
Umum Ekstrak Tumbuhan Obat, 1, 3, Fitokimia Ekstrak Buah Dengen.
Jakarta, Departemen Kesehatan Jurnal Dinamika, 8(1).
Rebublik Indonesia.
Jubaidah, S., Ria, I., Hayatus, S., Heri, W.,
Elda, M., Fatmawati., dan Santy, O., 2014, 2018, Formulasi Dan Uji
Ekstraksi Minyak Biji Kapuk Dengan Pertumbuhan Rambut Kelinci Dari
Metode Ekstraksi Soxhlet, Jurnal Sediaan Hair Tonic Kombinasi
Teknik Kimia, 20(1). Ekstrak Daun Seledri (Apium
graveolens Linn) Dan Daun
Fitriana, Apri Yudis., Retno, Wahyuningrum, Mangkokan (Polyscias scutellaria
Sudarso., 2012, Daya Repelan dan Uji (Burm.f.) Fosberg), Jurnal Ilmiah
Iritasi Formula Lotion Ekstrak Etanol Manuntung, 4(1).
Daun Sirih (Piper betle Linn) dengan
Variasi Basis Lanolin terhadap Nyamuk Kartadisastra, H.R., 1997, Ternak Kelinci,
Aedesaegypti,Pharmacy, 9(2). Penerbit Kanisius: Yogyakarta.

Febriani, A., Berna, E, & Mahdi, J., 2016, Uji Kamkaen, N., Phuntuwate, W., Samee, W.,
Akvitas dan Keamanan Hair Tonic Boonrod, A., & Treesak, C. 2007. The
12
 Halu Oleo Pharm.Sci. Vol. 1 No. 2
Investigation of the rabbit and Human
Skin Irritation Herbal Antiwrinkle
Cream. Vol. 2 (1). Thai Pharmaceutical
and Health Science Journal, 20-25.
Kusumadewi, 2011, Pengetahuan dan Seni
Tata Rambut Moderen, Jakarta: Meutia
Cipta Sarana & DPP, Tiara Kusuma.
Kristishanti,A., Phuntuwate, W., 2011,
Kemampuan Sediaan Hair Tonic Ekstrak
Kulit Apel (Malus Sylvestris L.) Var
Rome Beauty Dalam Menumbuhkan
Rambut Kelinci, Simposium Penelitian
Bahan Obat Alami XV dan Kongres
Obat Tradisional Indonesia IV.
Kartodimedjo, S, 2013, Cantik dengan
Herbal, Rahasia Puteri Keraton,
Yogyakarta, Citra Media.
Kuncari, Emma Sri., Iskandarsyahdan
Praptiwi., 2014, Evaluasi, Uji Stabilitas
Fisik dan Sineresis Sediaan Gel yang
Mengandung Minoksidil, Apigenin dan
Perasan Herba Seledri (Apium
graveolensL.), Bul. Penelit. Kesehat,
42(4).
Lestari, F. A., Formulasi Dan Evaluasi Fisik
Sediaan Hair Tonic Yang Mengandung
Ekstrak Etanol Biji Nangka (Artocarpus
heterophyllus. Lmk). Skripsi.
Mescher, A.L., 2010, Junqueira’s Basic
Histology Text & Atlas, New York: Mc
Graw Hill Medical.
Marchaban, Soegihardjo, C.J., dan
Kumarawati, F.E., 2010, Uji Aktivitas
Sari Daun Randu (Ceiba pentandra
Gaertn.) sebagai Penumbuh Rambut,
Fakultas Parmasi UGM, Yogyakarta.
Maurya, Pooja, 2017, Utilization Of Jackfruit
(Artocarpus heterophyllus lam.) Seed
For The Development Of Value Added
Products And Their Quality Evaluation,
Thesis, Maharana Pratap University Of
Agriculture And Technology.
13
Mukhriani, 2014, Ekstraksi, Pemisahan
Senyawa, dan Identifikasi Senyawa
Aktif, Jurnal Kesehatan, 7(2).
Nurjanah., Maria Krisnawati., 2014, Pengaruh
Hair Tonic Lidah Mertua (Sanseviera
Trifasciata Prain) Dan Seledri (Apium
Graveolens Linn)” Untuk Mengurangi
Rambut Rontok, Journal Of Beauty
And Beauty Health Education, 3 (1).
Nusmara, K.G., 2012, Uji Stabilitas Fisik dan
Aktivitas Pertumbuhan Rambut Tikus
Putih dari Sediaan Hair Tonic yang
Mengandung Ekstrak Etanol Daun Pare
(Momordica charantia), Skripsi,
Program Studi Farmasi FMIPA UI.
Depok.
Nusa, M.I., Misril.F dan Siti.F., 2014, Studi
Pengolahan Biji Buah Nangka Dalam
Pembuatan Minuman Instan, Agrium,
19 (1).
Pusponegoro, Erdina H.D, 2002, Kerontokan
Rambut Etiopatogenesis, Kesehatan
dan Keindahan Rambut. Jakarta.
Parker, K, 2010, The Rabbit Handbook,
Barron’s Aducational Series, New York.
Purnamas ari. D., 2013, Pengaruh Jumlah Air
Bongol Pisang klutuk Terhadap Sifat
Fisik dan Masa Simpan Hair Tonic
Rambut Rontok, e-Jurnal,
Surabaya,Universitas Negeri Surabay a.
Pratiwi, I., 2009, Uji Antibakteri Ekstrak Kasar
Daun Acalypha indica terhadap
Bakteri Salmonella choleraesuis dan
Salmonella typhimurium, Jurusan
Biologi FMIPA UNS, Surakarta.
Purwantini, 2013, Kombinasi Daun Teh Dan
Daun Mangkokan Sebagai Penumbuh
Rambut, Skripsi, Jogjakarta, Universitas
Gadjah Mada.
 Halu Oleo Pharm.Sci. Vol. 1 No. 2
Pratimasari, D., Nining, S., dan Nining, S.,
2015, Evaluasi Sifat Fisik dan Uji Iritasi
Sediaan Salep Minyak Atsiri Bunga
Cengkeh Dalam Basis Larut Air, Jurnal
Ilmiah Farmasi, 11(1).
PT. Surya Dermanto Medica Laboratories.
Surabaya. Indonesia.
Robinson T, 1995, Kandungan Organik
Tumbuhan Tinggi, Bandung: Institut
Teknologi Bandung.
Rowe, R.C., Sheskey, P.J., dan Quinn M.E.,
2009, Handbook of Pharmaceutical
Excipients sixth edition.Lexi-Comp:
American Pharmaceutical
Association,Ino, London:
Pharmaceutical Press.
Rahmawati, A., Sudarso., Hartanti, D., 2009,
Efek Hair Tonic Buah Mengkudu
(Morinda Citrifolia L) Dan Uji
Fitokimianya, Pharmacy, 1 (2).
Syamsuhidayat, S.S and Hutapea, J.R, 1991,
Inventaris Tanaman Obat Indonesia,
edisi kedua, Departemen Kesehatan RI,
Jakarta.
Soedibyo, B.R.A.M., dan Dalimartha, S.,
1998, Perawatan Rambut dengan
Tumbuhan Obat dan Diet Suplemen,
PT. Penebar Swadaya, Bogor.
Sitompul S., 2002, Kandungan Senyawa
Polifenol dalam Tanaman Lidah Buaya,
Daun Mimba, dan Ampas Buah
Mengkudu, Bogor: BPT Ciawi.
Soedirman, I., Ika, Y.A dan Kristanti., 2009,
Pengaruh Basis Salep Terhadap Sifat
Fisik dan Iritasi Primer Ekstrak Etanol
Jahe Merah (Zingiber officinale Roxb),
Pharmacy, 1(9).
Soepardiman, L., 2010, Kelainan Rambut,
Dalam: Djuanda, Adhi, dkk., Ilmu
Penyakit Kulit dan Kelamin, Jakarta:
14
Badan Penerbit Fakultas Kedokteran
Universitas Indonesia.
Sinaga, R., Sunny, W., dan Marie, M.K., 2012,
Peran Melanosit pada Proses Uban,
Jurnal Biomedik, 4(3).
Tanaka, S., M. Saito., Sabata M., 1980.
Bioassay of Crude Drugs for Hair
Growth Promoting Activity in Mice by A
New Simple Method, Journal of
Medicinal Plant Research.
Tranggono, S.R., 1992, Kiat-kiat Apik Tampil
Sehat dan Cantik, Gramedia Pustaka
Utama, Jakarta.
Tranggono, I.R., Latifah, 2007, Buku
Pegangan Ilmu Pengetahuan
Kosmetika, Jakarta, PT. Gramedia
Pustaka Utama.
Tambunan, Lidia Romito, 2012, Uji Stabilitas
Mikro Emulsi Ektrak Daun Seledri dan
Mikroemulsi Ekstrak Daun Urang Aring
dan Efektivitasnya Terhadap
Pertumbuhan Rambut Tikus Jantan
Spraque Dawley, Skripsi, Fakultas
Matematika dan Ilmu Pengetahuan
Alam Program Studi Farmasi.
Tejpal, A., and Parle, A., 2016, Jackfruit: A
Health Boon, Int. J. Res. Ayurveda
Pharm,7(3).
Umborowati, M.A dan Rahmadewi., 2012,
Rambut Rontok Akibat Lingkungan dan
Kosmetik (Environment and Cosmetic
Induced Hair Loss), Berkala Ilmu
Kesehatan Kulit & Kelamin, 24 (1).
Van Steenis, 2008, Flora, Cetakan ke-12.
Jakarta: PT. Pradnya Paramita.
Wade, A., and Weller, P.J., 1994, Handbook
of Pharmaceutical Excipients,
American Pharmaceutical Association,
London.
 Halu Oleo Pharm.Sci. Vol. 1 No. 2

Wasitaatmadja, S.M., 1997, Penuntun Ilmu

Kosmetik Medik, Cetakan , 202 -11,
Universitas Indonesia Press, Jakarta.

Zainuddin, N.S., 2018. Uji Aktivitas

Pertumbuhan Rambut Dari Ekstrak
Etanol Biji Nangka (Artocarpus
heterophyllus Lamk.) Terhadap
Hewan Coba Kelinci (Oryctolagus
cuniculus). Skripsi.

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