First City Providential College

Brgy. Narra, Francisco Homes Subdivision, City of San Jose del Monte, Bulacan

The Effectiveness of Guava Leaves Extract as Hand Sanitizer

A Research Paper Presented to

First City Providential College

In Partial Fulfillment to the requirements for the subject

Research in Daily Life

Research conducted by:

Mario R. Tingson Jr.

12-STEM 5

Submitted to:

Teacher Naomi Jane V. Baconawa

Academic Year 2019-2020

 CHAPTER 1

INTRODUCTION

Earlier, sanitation is one of the problems to the environment. Since the

infectious disease are caused by Pathogenic bacteria. The effectiveness of proper
hygiene is important staying healthy and clean gives a comfort to everyone. Hand
sanitizers are created to help kill bacteria in the body that people may cause
disease, however some sanitizers are based on a botanical ingredients.

Poor sanitation to the environment leads to illness caused by germs or

bacteria. It can cause much sickness and can lead to other health problems. Many
illnesses are spread from the person to person through touch or sometimes
through the objects that they use.

This study aims to inform and to help others to create a product which has
a cheaper but use as the same quality in the market. The Psidium Guavaja leaves
or the Guava leaves as hand sanitizer because of its benefits it contains vitamin C.
Guava leaves are easy to find in the local market and it has a cheaper price.
 STATEMENT OF THE PROBLEM

Main objective

The main objective of this research is to show the Guava leaves as hand sanitizer
and the benefits of it that can be useful.

Specific objective

This study will:

1. Determine the potential of Guava leaves extracts as hand sanitizer.

2. Analyze how effective is the Guava leaves as hand sanitizer.
3. Distinguish the characteristics of guava leaves.
 REVIEW OF RELATED LITERATURE

The Review of Related Literature shows how Guava leaves extract can
inhibit the bacteria and the Guava leaves might be a good for antibacterial agent.
Guava leaves are full of antioxidant, anti inflammatory agents, antibacterial.

Benefits of Guava According to Borelli, L. (2015) Guava leaves are just a

medicinally useful as the nutritional powerhouse fruit. The Guava leaves are full
of antioxidants, anti inflammatory agents, anti bacterial. Ojewole, J. Awe, E.
Chiworoco, (2008) Guava plant leaves are best known as a natural pain reliever,
they contain polyphenols, flavonoids and carotenoids which make them useful in
treating numerous disease and conditions for the local literature According to
Philippine Herbal Medicine (2005-2019) Bayabas or guava is a tropical plant,
which is locally known for its edible fruit. In the backyards of Filipino homes in
the country, this plant is commonly seen and grows because it’s many uses as
fruit and to treat ailment. Bayabas or Guava kills bacteria and fungi. Campaner
(2014 the leaves of Guava are rich in Flavonoids. The flavonoids have
demonstrated anti bacterial activity. Also contain an antioxidant property which is
attributed to the polyphenols.

The similarities of the benefits of guava leaves to the other keywords are it
can inhibit the growth of the bacteria. The differences of the benefits of guava
leaves to the other keywords are in benefits it focuses only in benefits of guava
leaves and in other keywords it shows the antibacterial activity and the
effectiveness.

Antibacterial of Guava leaves According to Biswas B. Rogers, k.

Mclaughin, F. Daniels, D. & Yadav A. (2013) anti-bacterial methanol and ethanol
extracts of Guava leaves showed inhibitory against bacteria Guava leaf extract
might be a good for anti-bacterial agent. Mailoa, Mahendradatta, Laga, Djid.
(2014) various types of plants contain natural preservatives as anti microbial and
anti oxidant compounds can inhibit the growth and have anti microbial activity.
Guava leaves is commonly used as a traditional medicine it may be anti microbial
inhibit the growth of bacteria. For the local literature According to Yaun, E.
Vasquez, B. (2017) Guava leaves is reducing growth rate of staphylococcus
aureus using guava leaf extract formulated into guava hand sanitizer gel. The leaf
of the Guava or Psidium guvaja, locally known as “bayabas” is a plant of the
family Myrtacea. Hwang EI, (2015) Studies that Flavonoid of citrus fruit is
essential oil have proved to be responsible for the antimicrobial activity.

The similarities of antibacterial of guava leaves to the other keywords it

shows that Guava leaves extract can be a good for antibacterial agent. The
differences to the antibacterial of guava leaves it focuses on the ethanol extract
against bacteria.

Effectiveness of Guava leaves According to Turner, Fuls, & Rodgers,

(2010) ethanol of Guava leaves as hand sanitizer from the hand to remove
detectable Rhinovirus were significant more effective.

The similarity of effectiveness of Guava leaves to the other keywords is

the effectiveness of Guava leaves of antibacterial properties and the positive
effects of it as hand sanitizer. The difference of the effectiveness is it focuses to
be main component of in hand sanitizer.

Therefore, the researcher concludes that the Guava leaves extract can
really help to remove and destroy germs to keep hands clean. The strength of the
Guava leaves extract is the properties of leaves that can inhibit the growth of
bacteria. The weaknesses of the review the researcher not completed the review of
related literature.
 REVIEW OF RELATED STUDIES

The Review of Related Study shows the benefits, antibacterial activity and
similarities and differences of Guava leaves extract as hand sanitizer.

According to Shauya, K, Renu, R. & Srinivas M. Study about plant parts

and foods are great interest nowadays due to their various health benefits. Guava
leaves are small evergreen tree belonging to the myrtic family. Guava leaves are
aromatic when it is crushed. Guava leaves extract was found properties like
antimicrobial. Shulman, L. (2006) several studies have concluded that the risk of
spreading gastrointestinal (stomach) and respiratory infection is decreased among
families who use hand sanitizers.

The similarities of the study that the benefit of Guava is it can be an

antibacterial agent. For the differences it can show other benefits like for the skin,
anti inflammatory.

According to Hwang EI, (2015) Studies that Flavonoid of citrus fruit is

essential oil have proved to be responsible for the antimicrobial activity.

The similarities of the study that antibacterial activity of Guava leaves

found properties of antibacterial. For the differences antibacterial it focuses on the
Flavonoid content of the leaves.
 Therefore, the researcher conclude that extraction of Guava leaves as
Hand sanitizer can be useful because of its benefits that can help to kill bacteria
and it is easy to use.
 REVIEW OF RELATED LITERATERATURE MATRIX

Foreign Literature

Author Keyword 1 Keyword 2 Keyword 3

Benefits of Guava Anti bacterial of Effectiveness of Guava
leaves Guava leaves leaves
Biswas B. Rogers, According to the
k. Mclaughin, F. findings of the
Daniels, D. & anti-bacterial
Yadav A. (2013) methanol and
ethanol extracts of
Guava leaves
showed inhibitory
against bacteria
Guava leaf extract
might be a good
for anti-bacterial
agent.
Mailoa, Various types of
Mahendradatta, plants contain
Laga, Djid. natural
(2014) preservatives as
anti microbial and
anti oxidant
compounds can
inhibit the growth
and have anti
microbial activity.
 Guava leaves are
commonly used
as a traditional
medicine it may
be anti microbial
inhibit the growth
of bacteria.

Borelli, L (2015) Guava leaves are

Just as
medicinally useful
as the nutritional
powerhouse fruit
they grow with.
The Guava leaves
of the Guava tree
are full of anti
oxidants, anti
inflammatory
agents, anti
bacterial.
Turner, Fuls, & Effectiveness of ethanol
Rodgers, (2010) of Guava leaves as hand
sanitizer from the hand
to remove detectable
Rhinovirus were
significant more
effective
Ojewole, J. Awe, Guava plant
E. Chiworoco, leaves are best
(2008) known as a
natural pain
reliever, they
contain
polyphenols,
flavonoids and
carotenoids which
make them useful
in treating
numerous disease
and conditions
Local Literature

Author Keyword 1 Keyword 2 Keyword 3

Benefits of Guava Anti bacterial of Effectiveness of
leaves Guava leaves Guava leaves
Yaun, E. Guava leaves is
Vasquez, B. reducing growth
(2017) rate of
staphylococcus
aureus using
guava leaf extract
formulated into
guava hand
sanitizer gel. The
leaf of the Guava
or Psidium
guvaja, locally
known as
“bayabas” is a
plant of the family
Myrtacea.
Philippine Herbal Bayabas or guava
Medicine (2005- is a tropical plant,
2019) which is locally
known for its
edible fruit. In the
backyards of
Filipino homes in
the country, this
plants is
 commonly seen
and grow because
its many uses as
fruit and to treat
ailment. Bayabas
or Guava kills
bacteria and
fungi.

Campaner (2014) The leaves of

Guava are rich in
Flavonoids. The
flavonoids have
demonstrated anti
bacterial activity.
Also contain an
antioxidant
property which is
attributed to the
polyphenols.
 REVIEW OF RELATED STUDIES MATRIX

Foreign Studies

Author Keyword 1 Keyword 2 Keyword 3

Benefits of Guava Anti bacterial of Effectiveness of
leaves Guava leaves Guava leaves
Shauya, K, Renu, Study about plant
R. & Srinivas M. parts and foods
are great interest
nowadays due to
their various
health benefits.
Guava leaves are
small evergreen
tree belonging to
the myrtic family.
Guava leaves are
aromatic when it
is crushed. Guava
leaves extract was
found properties
like antimicrobial
Shulman, L. Several studies
(2006) have concluded
that the risk of
spreading
gastrointestinal
(stomach) and
respiratory
infection is
 decreased among
families who use
hand sanitizers.

Hwang EI, (2015) Studies that

Flavonoid of
citrus fruit is
essential oil have
proved to be
responsible for
the antimicrobial
activity.
 SIGNIFICANCE OF THE STUDY

This study is significant in proving the Effectiveness of Guava Leaves Extract

as Hand Sanitizer. It is also beneficial to the following.

 Students. The Guava leaves sanitizer will help them to kill the bacteria in their
hands. It is easy to find in the local market and it is easy to use.
 Teachers. The Guava leaves sanitizer will help the teachers because when they
are using Chalk, after that they can easily use the hand sanitizer to remove the
bacteria in their hands.
 Consumers. The consumers will easily find the hand sanitizer in the local market
and it has a cheaper price.
 Future Research. They will be informed to create a product which has a cheaper
price but it has the same quality in the market.

SCOPE AND LIMITATION

This study aims to know how effective the Guava leaves extract as hand
sanitizer that can be useful and beneficial to everyone’s health to help kill bacteria
in the body that people may cause diseases. This will be conducted from the
month of November to 2019 to February 2020, in the researcher’s residence at
Blk. 3 Lot 4A Phase M Brgy. Guijo City of San Jose Del monte Bulacan. This
research only focuses on determining the potential of Guava leaves extract as
hand sanitizer that can kill the bacteria in hands. It also limits its study only on the
Guava leaves are the botanical ingredients in using hand sanitizer.
 DEFINITION OF TERMS

Hand Sanitizer- is a liquid generally used to remove infectious agents on the

hands.

Bacteria- are microscopic, single-celled organisms that diverse in environment.

Antioxidants- prevent skin damage from the inside out.

Flavonoids- are diverse group of phytonutrients (plant chemicals) found in almost

all fruits and Vegetables.

Guava leaves- are oblong to oval in shape and average 7-15 centimeters long and
3-5 centimeters wide. Guava leaves are aromatic when crushed and leathery with
faint white veins.

HYPOTHESIS

1. The Guava leaves extract can be an alternative hand sanitizer

2. There is a significant difference between the Guava leaves and the other botanical
ingredients that can be use as hand sanitizer.
 CONCEPTUAL FRAMEWORK

The Effectiveness of Guava Leaves

Extract as Hand sanitizer

Independent Dependent

Guava Leaves Hand Sanitizer

extract

THEORETICAL FRAMEWORK

Plant extraction is a process that aims to extract certain components present in

plants. It is a solid and liquid separation: a solid object (the plant) is placed in
contract with a fluid (the solvent). The plant components of interest are then
solubilised and contained within the solvent. The solution thus obtained is the
desired extract.

In this process of extracting it shows the content of the Guava leaves will be
release. It depends on the operation whether solid separation or liquid separation.
The essential oils are the liquids that are isolated form plants when in introduced
to solvents they are liquefied versions of the plants.
 CHAPTER 2: METHODOLOGY

I. Research design

An experimental research study about the effectiveness of guava leaves

extract as hand sanitizer which is a quantitative research is used in this study. This
type of research allows for greater objectivity and accuracy of the results.

The research method in this study is the reduction of bacteria using the
guava leaves extract compared to the other brands of hand sanitizer containing
alcohol.

II. Research Setting

The quantitative research was done in the researcher’s residence at Blk. 3

Lot 4A Phase M Brgy. Guijo City of San Jose Del monte Bulacan. This research
determine the guava leaves by extracting that can be useful to everyone health.
This will be conducted form the month of November 2019 to February 2020.

III. Data Gathering/Procedure

Materials:

In this study, the material that was used is the dried 50g Guava leaves,
150ml of ethanol with a concentration of 30%, 50%, and 70%, distilled water,
filter paper, blender, and sieve.
 Procedure:

The fresh leaves of the Guava was collected and washed to remove
unwanted contaminants and other particles which may affect in determining
microbial activity of the plant. It needs to be cut into smaller pieces 50g of Guava
leaves will be soaked in 150ml of ethanol with concentration of 30%, 50%, and
70% for 24hrs and then filtered to obtain a filtrate. The filtrate was evaporated
until it reached a syrupy consistency.

Time:

This study aims to provide a Guava leaves extract at the researchers

residence to determine if extraction of Guava leaves is effective as a hand
sanitizer that can kill bacteria. The product is estimated to be done within 2 days.