II.

MATERIAL AND METHODS

A. Material

The materials that used in this practice freshwater fish; nilem fish (Osteochilus
vittatus), nila fish (Oreochromis niloticus), KOH-KI, H2SO4, Na2S2O3, MnSO4, and
amylum reagent.
The tools that used in this practice are aerator, technical scales, respirometer,
erlenmeyer, Winkler bottle, burette, statif, and measuring cup.

B. Methods

1. Respirator is being run, the water pump is being turned on for 15 minutes.
2. The weight and volume of fish are measured (Vfish = Vtotal – V1(initial))
3. The fish is put into the respirometer, no bubbles inside are made sure.
4. The water sample is taken with Winkler bottle (250 mL).
5. The solutions are added
a) MnSO4 1 mL
b) KOH-KI 1 mL
c) H2SO4 1 mL
6. The solution is taken into the erlenmeyer (100 mL), 2-3 drops of amylum is
added.
7. The early titration with Na2S2O3 is done.
8. After 15 minutes, the water sample is taken with Winkler bottle (250 mL).
9. The final titration is done.
10. The consumption of O2 is calculated.
1000
Ota/Otak = ×p×q×8
100
VO2= (cO2i - cO2f) × V × H-1 × W-1