Standard/sample concentration Absorbance 1 Absorbance 2

std 0 0 0.0047 0.0116

std 200 200 0.1942 0.1674
std 400 400 0.3479 0.348
(1) Whole body without tunic 0.3087 0.3600
(2) Coelomic fluid 0.2138 0.2058

Chart Title
0.4
0.35
f(x) = 0.0008495x + 0.0090666667
0.3 R² = 0.9999126788
0.25
0.2
Axis Title
0.15
0.1
0.05
0
0 50 10015020025030035040045
Axis Title
 Average absorbance Base concentration Dilution factor Volume of Sample
Vanadium concentration in the sample tube

0.00815
0.1808
0.34795
0.33435 406.563 60 2.416 24393.750
0.2098 250.875 40 6.076 10035.000

Chart Title

f(x) = 0.0008495x + 0.0090666667

R² = 0.9999126788
Column E
Linear (Column E)

0 50 100150200250300350400450
Axis Title
Amount of vanadium in the sample tube (ng)wet weight of biological material (g)

29467.65 1.208
30787.38 3.068

0
Vanadium concentration perwet sample (ng/g=ppb)

24393.75
10035
 Standard/sample concentration Absorbance
Absorbance
1 Average
2 absorbance
std 0 0 0.0047 0.0116 0.00815
std 200 200 0.1942 0.1674 0.1808
std 400 400 0.3479 0.348 0.34795
(1) Whole body without tunic 0.3087 0.3600 0.33435
(2) Coelomic fluid 0.2138 0.2058 0.2098