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Biochem Problem Set For The First Shifting
Biochem Problem Set For The First Shifting
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3. What is the pH of the buffer 0.1OIvl the pH of the solution.
NazHPO+/O.15M KH2PO4? Kur=7.5 x 10-3, 11. How much H3PO4 and NaOH are needed to
Kaz=6.2O x 10-8, K"3=4.8 x 10-13. prepare a buffer solutign containing 0.200 mole
4. What is the pH of a 500. mL buffer HPO42- and 0.500 mole POo3-z
containing.0.250 moles HCO2H and 0.300 12. Describe the preparation of 500. mL 0.250M
phosphate buffer, pH=11.5, frirm 6M NaOH and
moles HCO2Na? Ka=1.70 x 10-4.
5M H3PO4.
5. What is the final pH of a solution made b Y 13. What initial concentration of aqueous sodium
mixing 100 ml of 0.05 M acetic acid and 100 dihyd phosphate and aqueous sodium
ml of 0.1 M sodium acetate? hvd ed to pr:epare 500 mL
6. Refer to the .following buffers and the a buffer
corres ponding pK" of the acid: "' .14. Propionic has a K" of ,1.3 x 1,0-
A
sodium hydrogen citrateJdGodium- hydE[en of sodium p ropionate,
citrate 4.76 CH3CH2COONa, to be ad ded to 1.00 L
D boric acid/ primary sodlum borate 9.23 of 0.500 mol.L-1 acid,for a resulting
C sod iu na!iqq rbonate/sod tum ca rbonate 10.93 solution of pH 5 .00?
n potassium dihydrogen phosphate/ disodium 15. What is the pH of the buffer prepared by mixinrg
hydroqenpho5phate v$a90.t l\&rHPO,l 7.2r 250 mL of 0.20 0 M benzoic acid and 100. mL of
tris( hyd roxymethyl )a m inomethane 0.250M NaOH? p Ka=4.19
E hydrochloride/ 8.30 16. Prepare 200. mL of a 0.100 M formate buffer,
tris(hydroxymethy[)a minomethane
ph=4.50, from a 0.200 M stock soln' of formic
acid and its salt. Ka=4.27x10-7.
Suggest a conjugate bcid-base pair that could be 17. At the physiological pH of 7.4, what is the ratio of
use to prepare the buffer with the given pH. salt concentration to acid concentration for a
Calculate also the ratio of salt to that of the HPO4-. and Fl2POo- buffering system if the pKa is
acid: 6.8? Which:is the acid in this system?
18. Prepare 500. rnl of 0.100 M bicarbonate buffer,
pl.l=6.00, from 0.100 M stock soln' of carbonic
pH Buffer Isalt] / [acid] acid and its salt. K^=4.27x7A't.
9.41 19. Calculate the pH change that take place when a
100. nrl portion of (a) 0.0500 M NaOH a nd (b)
7.00 0.0500 It1 HCI is to 400. m of the buffer ,
sdi'n-Glii€i?ffif o
6. Suggest a suitable buffer range for each of Ku:5.70x10-10.
the following substances: 20. A buffer containing 1.00 M conceRtratioris.'of HOAc
A. and NaOAc has a pH of 4.742. (a) What is the pH
Lactic acid'(pKu=3.86) & its sodium sall:
of the soln' after 0.0100 mol of HCI has been
added to 1.00 L of the buffer? (b) What is the pH
B. HEPES (PK"=8.30) in its zwitterionic forrn of the soln' after the addition of 0.0100 mol of
& its anionic form: NaOH? (c) What is the buffer capacity and pH
change for each soln' I in (a) and (b)]?
7. Describe the preparation of a 200. mL of a 0.200 M 21. A buffered solution of pH = 4.4 is 0.1 M
benzoate buffer, pH=4.7 if your starting materials benzoic Acid, HC7H5O2, EDd 0.15 M sodium
are 80.0 mL of 0.200 M C6H5COONa and 10.0 g benzoate, NaC7H5O2. After addition of 1.5 mL
CGHsCOOH (122.73 g/mol). pKa=4.20.
8. Describe the preparation of 500. mL of 0.300 M of 6.0 M HCI to 250 mL of the buffered
acetate buffer, pH=5.0, from 0.300 M NaOAc and
solution, what is the :resulting pH?:
0.300 M HOAc. Ku=1.8x10-s. K"= 6'5 x 1o-s
9. Describe the preparation of 500. mL acetate buffer, 22. What will be lhe ratio of acid-base pair of
pH=5.0 from solid NaOAc (10.0g) and stock soln, 0.115 M glycine solution that has a pH of
of HoAc (95o/o w/w,p=1.0498). 8.10? What is the charge of the predominant
10. What are the components of the buffer solution form of glycine in the solution? pKar=2.49,
when: i
PKa2=9 gg
[a] 0.30 mol H3pOo is mixed with 23. If i.oo *t of o.0go M HCI was added to
0.10 mol NaOH 0.100 L glycine solution, what will be the
lbl 0.20 mol H3POo is mixed with new pH of the solution? What is the bufFer
0.30 mol of the base
capacrty?
[c] 0.ZO mol H3POa is made to react
with 0.40 mol of the base 24. Can a buffer be prepared by adding 5.0 g of
[d] 65.0 g potassium primary glycine to 0.150 L of 1.04 M NaOH? Justify
phosphate is made to react with your answer.
200.0 mL of 3M NaOH. Calculate