Cell Lines Human Epidermal keratinocytes (psoriatic Model)

Control Concentration (μg/ml)

OD 1 OD 2 OD 3 % of cell death
0.619 250.00 0.176 0.182 0.173 71.66 70.69 72.14
0.623 125.00 0.243 0.253 0.251 60.87 59.26 59.58
0.621 62.50 0.289 0.29 0.283 53.46 53.30 54.43
0.621 31.25 0.318 0.32 0.312 48.79 48.47 49.76
0.002 15.63 0.389 0.334 0.33 37.36 46.22 46.86
0.001 7.81 0.449 0.451 0.443 27.70 27.38 28.66
3.91 0.525 0.533 0.529 15.46 14.17 14.81
1.95 0.603 0.61 0.619 2.90 1.77 0.32

Non Linear regression Curve fit Cell Viability (%)

150 150

100

Cell viability (%)

Response

100

50
50

0
0 100 200 300
Log dose 0

50

25

.5
25

10

12
Concentration 
 250 ug/mL
Mean SD SEM % Live cell
71.50 0.74 0.43 28.50
59.90 0.85 0.49 40.10
53.73 0.61 0.35 46.27
49.01 0.67 0.39 50.99
43.48 5.31 3.07 56.52
27.91 0.67 0.39 72.09
14.81 0.64 0.37 85.19
1.66 1.29 0.75 98.34

Cell Viability (%)

Normal Human Epidermal Keratinocytes

0

2
50

25

.5

25
25

10

12

56
12

6.

3.

1.

Concentration g/ml
 125ug/mL 62.50 ug/ml

mal Keratinocytes
 150 (a) Global (shared)
Comparison of Fits Can't calculate
Null hypothesis Different curve for each data set
Alternative hypothesis One curve for all data sets
P value
Conclusion (alpha = 0.05) Models have the same DF
Preferred model Different curve for each data set
F (DFn, DFd)

Different curve for each data set

Best-fit values
Bottom = 0.0
Top = 100.0
LogIC50 1.074
HillSlope -1.02
IC50 11.86
Span = 100.0
Std. Error
LogIC50 0.06463
HillSlope 0.1495
95% Confidence Intervals
LogIC500.9159 to 1.232
HillSlope-1.386 to -0.6539
IC50 8.239 to 17.07
Goodness of Fit
Degrees of Freedom 6
R² 0.9605
Absolute Sum of Squares317.3
Sy.x 7.272
Constraints
Bottom Bottom = 0.0
Top Top = 100.0

One curve for all data sets

Best-fit values
Bottom = 0.0
Top = 100.0
LogIC50 1.074 1.074
HillSlope -1.02 -1.02
IC50 11.86 11.86
Span = 100.0
Std. Error
LogIC50 0.06463 0.06463
HillSlope 0.1495 0.1495
95% Confidence Intervals
LogIC500.9159 to 1.232 0.9159 to 1.232
HillSlope
-1.386 to -0.6539 -1.386 to -0.6539
IC50 8.239 to 17.07 8.239 to 17.07
 Goodness of Fit
Degrees of Freedom 6
R² 0.9605 0.9605
Absolute Sum of Squares317.3 317.3
Sy.x 7.272
Constraints
Bottom Bottom = 0.0
Top Top = 100.0
LogIC50LogIC50 is shared
HillSlope
HillSlope is shared
Number of points
Analyzed 8