Sample

Name (-)

Standard Total Mass of Flask (g) A1

Stock
Solution Species 1 Species 2 Species 3 A1
Empty Container (g)
MeOH (g) iPrOH (g) nBuOH (g) A1
102.181 - 199.185 A2
A 108.091 Mass frac 1 Mass frac 2 Mass frac 3 A2
0.34 0.00 0.66 A2
- 195.573 - A3
B 119.002 Mass frac 1 Mass frac 2 Mass frac 3 A3
0.00 1.00 0.00 A3
MeOH nBuOH
IPrOH (g/mol) A4
(g/mol) (g/mol)
32.04 60.1 74.123 A4
A4
MeOH A5
0.35 A5
0.30 f(x) = 0.0117517295x + 0.0009795264 A5
R² = 0.9056905975
A6
0.25
A6
0.20
Mass %

A6
0.15 B1
0.10 B1
0.05 B1
0.00 B2
0.00 5.00 10.00 15.00 20.00 25.00 30.00 B2
% Area B2
B3
B3
iPrOH B3
1.00 B4
0.90 B4
f(x) = 0.009955231x - 0.0058209189
0.80 R² = 0.9991388175 B4
0.70
0.60 B5
Mass %

0.50 B5
0.40 B5
0.30 B6
0.20
0.10
B6
0.00
0.00 10.00 20.00 30.00 40.00 50.00 60.00 70.00 80.00 90.00 100.00
% Area
 0.50

Mas
0.40
0.30
0.20
0.10
0.00 B6
0.00 10.00 20.00 30.00 40.00 50.00 60.00 70.00 80.00 90.00 100.00
C1
% Area C1
C1
C2
nBuOH C2
0.70 C2
f(x) = 0.0087739713x + 0.0246640278 C3
0.60 R² = 0.9854324852
0.50
C3
C3
0.40
Mass %

C4
0.30
C4
0.20 C4
0.10 C5
0.00 C5
0.00 10.00 20.00 30.00 40.00 50.00 60.00 70.00 80.00
C5
% Area C6
C6
C6
D1
D1
D1
D2
D2
D2
D3
D3
D3
D4
D4
D4
D5
D5
D5
D6
D6
D6
 Sum of Species 1 Species 2 Species 3
Species 1 Species 2 Species 3
Trial (#) Corrected Pct Area Pct Area Pct Area
Area (-) Area (-) Area (-)
Area (-) (%) (%) (%)

1 12593985 443554586 33305231 489453802 2.57 90.62 6.80

2 12837797 452769742 33965765 499573303 2.57 90.63 6.80

3 13660856 482456624 36280219 532397699 2.57 90.62 6.81
1 39296438 400945263 81770414 522012115 7.53 76.81 15.66
2 40378605 412532706 84286681 537197991 7.52 76.79 15.69
3 41370628 422919181 86462881 550752689 7.51 76.79 15.70
1 86477525 282049721 176601434 545128681 15.86 51.74 32.40
2 89688677 292149027 182652185 564489889 15.89 51.75 32.36
3 80937616 264186931 165572535 510697082 15.85 51.73 32.42

1 111333317 155387606 311645228 578366151 19.25 26.87 53.88

2 109025745 152195807 305393456 566615008 19.24 26.86 53.90
3 103581306 144915314 291464945 539961565 19.18 26.84 53.98
1 163487446 65104181 363549214 592140841 27.61 10.99 61.40
2 158532950 62958467 350906766 572398183 27.70 11.00 61.30
3 150213076 59689304 333250527 543152908 27.66 10.99 61.35
1 132310331 161373349 271843468 565527149 23.40 28.54 48.07
2 139056757 169708037 285724220 594489013 23.39 28.55 48.06
3 133723040 163252185 275524617 572499842 23.36 28.52 48.13
1 12818326 450970814 28078009 491867149 2.61 91.69 5.71
2 13770526 484873117 30304267 528947910 2.60 91.67 5.73
3 13322257 468748651 29202778 511273686 2.61 91.68 5.71
1 45318133 351877176 97951591 495146901 9.15 71.07 19.78
2 47142449 366572888 102212157 515927494 9.14 71.05 19.81
3 47956572 373141005 104114112 525211689 9.13 71.05 19.82
1 85479506 312174600 199854407 597508513 14.31 52.25 33.45
2 74678918 272924403 174844313 522447634 14.29 52.24 33.47
3 76169081 278021542 177339088 531529711 14.33 52.31 33.36
1 119126590 162762212 244322171 526210972 22.64 30.93 46.43
2 127765494 174298138 261480629 563544261 22.67 30.93 46.40
3 127570387 174106523 261634602 563311512 22.65 30.91 46.45
1 103792957 52481238 434481742 590755937 17.57 8.88 73.55
2 103171122 52261190 432813565 588245878 17.54 8.88 73.58
3 104969464 53322852 442768316 601060633 17.46 8.87 73.66
1 126467974 166958900 285050782 578477656 21.86 28.86 49.28
2 126515268 167303124 285722378 579540769 21.83 28.87 49.30
3 126570594 167516445 286287126 580374165 21.81 28.86 49.33
1 41487947 398746000 88867036 529100983 7.84 75.36 16.80
2 37853321 363860923 80842370 482556615 7.84 75.40 16.75
3 40044493 384865821 85581101 510491415 7.84 75.39 16.76
1 54908626 344730140 127646468 527285235 10.41 65.38 24.21
2 54480439 341394013 126422833 522297286 10.43 65.36 24.21
3 52871037 331181626 122944248 506996911 10.43 65.32 24.25
1 79356993 259220657 173913369 512491020 15.48 50.58 33.93
2 80041052 260874420 174299428 515214901 15.54 50.63 33.83
3 80041664 261223924 174941028 516206616 15.51 50.60 33.89
1 114044099 222443332 234548733 571036165 19.97 38.95 41.07
2 104039971 202743335 213594246 520377552 19.99 38.96 41.05
3 114628587 223320277 235299865 573248729 20.00 38.96 41.05
1 118668107 148144195 297801354 564613655 21.02 26.24 52.74
2 119977499 150285000 303237189 573499689 20.92 26.20 52.87
3 117102719 146734316 295649790 559486825 20.93 26.23 52.84
1 131469991 165123063 279580221 576173275 22.82 28.66 48.52
2 122046559 153314785 259600475 534961818 22.81 28.66 48.53
3 119786312 150473249 254797270 525056830 22.81 28.66 48.53
1 13269534 461840190 33691432 508801155 2.61 90.77 6.62
2 12960790 452210909 33059663 498231361 2.60 90.76 6.64
3 13091169 455752471 33303524 502147164 2.61 90.76 6.63
1 48800405 373123522 105100437 527024364 9.26 70.80 19.94
2 47761947 365347454 102916409 516025810 9.26 70.80 19.94
3 50372583 385369971 108705963 544448517 9.25 70.78 19.97
1 83643640 275897511 193097255 552638406 15.14 49.92 34.94
2 77038060 254625332 178365334 510028727 15.10 49.92 34.97
3 76822634 253422724 177121130 507366487 15.14 49.95 34.91
1 128689966 163208667 290454936 582353568 22.10 28.03 49.88
2 119095546 151023248 268856496 538975290 22.10 28.02 49.88
3 119483364 151509606 269855374 540848343 22.09 28.01 49.89
1 136027923 68029423 385370586 589427932 23.08 11.54 65.38
2 125288941 62642403 354425818 542357162 23.10 11.55 65.35
3 136395649 68413162 387918389 592727200 23.01 11.54 65.45
1 132796596 161190162 270481542 564468301 23.53 28.56 47.92
2 134753591 163670804 274912685 573337080 23.50 28.55 47.95
3 132467576 160609334 269430911 562507821 23.55 28.55 47.90
 Vial Vial Vial
Total Area Empty Vial Volume Volume Volume
Weight A Weight B Weight C
(%) Weight (g) Std A (µl) Std B (µl) Std C (µl)
(g) (g) (g)

100.00 2.242 100 2.313 900 2.971 - -

100.00 2.242 100 2.313 900 2.971 - -

100.00 2.242 100 2.313 900 2.971 - -
100.00 2.228 300 2.429 700 3.01 - -
100.00 2.228 300 2.429 700 3.01 - -
100.00 2.228 300 2.429 700 3.01 - -
100.00 2.247 500 2.627 500 3.028 - -
100.00 2.247 500 2.627 500 3.028 - -
100.00 2.247 500 2.627 500 3.028 - -

100.00 2.242 700 2.774 300 2.958 - -

100.00 2.242 700 2.774 300 2.958 - -
100.00 2.242 700 2.774 300 2.958 - -
100.00 2.254 900 2.994 100 3.083 - -
100.00 2.254 900 2.994 100 3.083 - -
100.00 2.254 900 2.994 100 3.083 - -
100.00 2.221 - - - - 1000 2.993
100.00 2.221 - - - - 1000 2.993
100.00 2.221 - - - - 1000 2.993
100.00 2.243 100 2.312 900 2.989 - -
100.00 2.243 100 2.312 900 2.989 - -
100.00 2.243 100 2.312 900 2.989 - -
100.00 2.217 300 2.458 700 2.984 - -
100.00 2.217 300 2.458 700 2.984 - -
100.00 2.217 300 2.458 700 2.984 - -
100.00 2.239 500 2.573 500 2.949 - -
100.00 2.239 500 2.573 500 2.949 - -
100.00 2.239 500 2.573 500 2.949 - -
100.00 2.216 700 2.761 300 2.983 - -
100.00 2.216 700 2.761 300 2.983 - -
100.00 2.216 700 2.761 300 2.983 - -
100.00 2.247 900 2.857 100 2.912 - -
100.00 2.247 900 2.857 100 2.912 - -
100.00 2.247 900 2.857 100 2.912 - -
100.00 2.181 - - - - 1000 2.974
100.00 2.181 - - - - 1000 2.974
100.00 2.181 - - - - 1000 2.974
100.00 2.231 100 2.545 900 3.478 - -
100.00 2.231 100 2.545 900 3.478 - -
100.00 2.231 100 2.545 900 3.478 - -
100.00 2.248 300 2.675 700 3.438 - -
100.00 2.248 300 2.675 700 3.438 - -
100.00 2.248 300 2.675 700 3.438 - -
100.00 2.222 500 2.847 500 3.468 - -
100.00 2.222 500 2.847 500 3.468 - -
100.00 2.222 500 2.847 500 3.468 - -
100.00 2.239 700 2.994 300 3.466 - -
100.00 2.239 700 2.994 300 3.466 - -
100.00 2.239 700 2.994 300 3.466 - -
100.00 2.186 900 3.115 100 3.43 - -
100.00 2.186 900 3.115 100 3.43 - -
100.00 2.186 900 3.115 100 3.43 - -
100.00 2.187 - - - - 1000 3.209
100.00 2.187 - - - - 1000 3.209
100.00 2.187 - - - - 1000 3.209
100.00 2.212 100 2.282 900 2.97 - -
100.00 2.212 100 2.282 900 2.97 - -
100.00 2.212 100 2.282 900 2.97 - -
100.00 2.234 300 2.459 700 2.992 - -
100.00 2.234 300 2.459 700 2.992 - -
100.00 2.234 300 2.459 700 2.992 - -
100.00 2.203 500 2.588 500 2.954 - -
100.00 2.203 500 2.588 500 2.954 - -
100.00 2.203 500 2.588 500 2.954 - -
100.00 2.215 700 2.74 300 2.936 - -
100.00 2.215 700 2.74 300 2.936 - -
100.00 2.215 700 2.74 300 2.936 - -
100.00 2.185 900 2.769 100 2.847 - -
100.00 2.185 900 2.769 100 2.847 - -
100.00 2.185 900 2.769 100 2.847 - -
100.00 2.226 - - - - 1000 2.99
100.00 2.226 - - - - 1000 2.99
100.00 2.226 - - - - 1000 2.99
 Mass MeOH Mass iPrOH Mass frac
Mass A (g) Mass B (g) Mass C (g) Mass nBuOH (g)
(g) (g) MeOH

0.071 0.658 - 0.024 0.658 0.047 0.03

0.071 0.658 - 0.024 0.658 0.047 0.03

0.071 0.658 - 0.024 0.658 0.047 0.03
0.201 0.581 - 0.068 0.581 0.133 0.09
0.201 0.581 - 0.068 0.581 0.133 0.09
0.201 0.581 - 0.068 0.581 0.133 0.09
0.38 0.401 - 0.129 0.401 0.251 0.16
0.38 0.401 - 0.129 0.401 0.251 0.16
0.38 0.401 - 0.129 0.401 0.251 0.16

0.532 0.184 - 0.180 0.184 0.352 0.25

0.532 0.184 - 0.180 0.184 0.352 0.25
0.532 0.184 - 0.180 0.184 0.352 0.25
0.74 0.089 - 0.251 0.089 0.489 0.30
0.74 0.089 - 0.251 0.089 0.489 0.30
0.74 0.089 - 0.251 0.089 0.489 0.30
- - 0.772 - - - -
- - 0.772 - - - -
- - 0.772 - - - -
0.069 0.677 - 0.023 0.677 0.046 0.03
0.069 0.677 - 0.023 0.677 0.046 0.03
0.069 0.677 - 0.023 0.677 0.046 0.03
0.241 0.526 - 0.082 0.526 0.159 0.11
0.241 0.526 - 0.082 0.526 0.159 0.11
0.241 0.526 - 0.082 0.526 0.159 0.11
0.334 0.376 - 0.113 0.376 0.221 0.16
0.334 0.376 - 0.113 0.376 0.221 0.16
0.334 0.376 - 0.113 0.376 0.221 0.16
0.545 0.222 - 0.185 0.222 0.360 0.24
0.545 0.222 - 0.185 0.222 0.360 0.24
0.545 0.222 - 0.185 0.222 0.360 0.24
0.61 0.055 - 0.207 0.055 0.403 0.31
0.61 0.055 - 0.207 0.055 0.403 0.31
0.61 0.055 - 0.207 0.055 0.403 0.31
- - 0.793 - - - -
- - 0.793 - - - -
 - - 0.793 - - - -
0.314 0.933 - 0.106 0.933 0.208 0.09
0.314 0.933 - 0.106 0.933 0.208 0.09
0.314 0.933 - 0.106 0.933 0.208 0.09
0.427 0.763 - 0.145 0.763 0.282 0.12
0.427 0.763 - 0.145 0.763 0.282 0.12
0.427 0.763 - 0.145 0.763 0.282 0.12
0.625 0.621 - 0.212 0.621 0.413 0.17
0.625 0.621 - 0.212 0.621 0.413 0.17
0.625 0.621 - 0.212 0.621 0.413 0.17
0.755 0.472 - 0.256 0.472 0.499 0.21
0.755 0.472 - 0.256 0.472 0.499 0.21
0.755 0.472 - 0.256 0.472 0.499 0.21
0.929 0.315 - 0.315 0.315 0.614 0.25
0.929 0.315 - 0.315 0.315 0.614 0.25
0.929 0.315 - 0.315 0.315 0.614 0.25
- - 1.022 - - - -
- - 1.022 - - - -
- - 1.022 - - - -
0.07 0.688 - 0.024 0.688 0.046 0.03
0.07 0.688 - 0.024 0.688 0.046 0.03
0.07 0.688 - 0.024 0.688 0.046 0.03
0.225 0.533 - 0.076 0.533 0.149 0.10
0.225 0.533 - 0.076 0.533 0.149 0.10
0.225 0.533 - 0.076 0.533 0.149 0.10
0.385 0.366 - 0.131 0.366 0.254 0.17
0.385 0.366 - 0.131 0.366 0.254 0.17
0.385 0.366 - 0.131 0.366 0.254 0.17
0.525 0.196 - 0.178 0.196 0.347 0.25
0.525 0.196 - 0.178 0.196 0.347 0.25
0.525 0.196 - 0.178 0.196 0.347 0.25
0.584 0.078 - 0.198 0.078 0.386 0.30
0.584 0.078 - 0.198 0.078 0.386 0.30
0.584 0.078 - 0.198 0.078 0.386 0.30
- - 0.764 - - - -
- - 0.764 - - - -
- - 0.764 - - - -
 MeOH iPrOH nBuOH
Mass frac Mass frac Sum Calc. Area
CONC CONC CONC
iPrOH nBuOH Check MeOH
(mM) (mM) (mM)

0.90 0.06 1.00 3.48

6.1E+04 8.9E+05 5.1E+04
0.90 0.06 1.00 1.6E+05 7.1E+05 1.3E+05 8.26
0.90 0.06 1.00 2.9E+05 4.7E+05 2.4E+05 14.71
0.74 0.17 1.00 4.2E+05 2.3E+05 3.5E+05 21.38
0.74 0.17 1.00 4.9E+05 9.3E+04 4.1E+05 25.04
0.74 0.17 1.00 3.9E+05 2.6E+05 3.5E+05 19.85
0.51 0.32 1.00 5.8E+04 8.9E+05 4.9E+04 3.32
0.51 0.32 1.00 1.9E+05 6.5E+05 1.6E+05 9.91
0.51 0.32 1.00 2.8E+05 4.9E+05 2.3E+05 14.27

0.26 0.49 1.00 20.57

4.0E+05 2.6E+05 3.4E+05
0.26 0.49 1.00 5.0E+05 7.1E+04 4.2E+05 25.63
0.26 0.49 1.00 3.7E+05 2.6E+05 3.6E+05 19.08
0.11 0.59 1.00 1.5E+05 7.2E+05 1.3E+05 8.10
0.11 0.59 1.00 2.1E+05 6.0E+05 1.8E+05 11.18
0.11 0.59 1.00 2.9E+05 4.6E+05 2.5E+05 15.11
- - - 3.5E+05 3.5E+05 3.0E+05 18.12
- - - 4.2E+05 2.2E+05 3.5E+05 21.47
- - - 3.9E+05 2.6E+05 3.6E+05 19.74
0.91 0.06 1.00 5.8E+04 8.9E+05 4.9E+04 3.32
0.91 0.06 1.00 1.8E+05 6.7E+05 1.5E+05 9.41
0.91 0.06 1.00 3.0E+05 4.5E+05 2.5E+05 15.41
0.69 0.21 1.00 4.1E+05 2.4E+05 3.5E+05 21.01
0.69 0.21 1.00 4.9E+05 1.0E+05 4.1E+05 24.79
0.69 0.21 1.00 4.0E+05 2.6E+05 3.5E+05 20.21
0.53 0.31 1.00
0.53 0.31 1.00
0.53 0.31 1.00
0.29 0.47 1.00
0.29 0.47 1.00
0.29 0.47 1.00
0.08 0.61 1.00
0.08 0.61 1.00
0.08 0.61 1.00
- - -
- - -
 - - -
0.75 0.17 1.00
0.75 0.17 1.00
0.75 0.17 1.00
0.64 0.24 1.00
0.64 0.24 1.00
0.64 0.24 1.00
0.50 0.33 1.00
0.50 0.33 1.00
0.50 0.33 1.00
0.38 0.41 1.00
0.38 0.41 1.00
0.38 0.41 1.00
0.25 0.49 1.00
0.25 0.49 1.00
0.25 0.49 1.00
- - -
- - -
- - -
0.91 0.06 1.00
0.91 0.06 1.00
0.91 0.06 1.00
0.70 0.20 1.00
0.70 0.20 1.00
0.70 0.20 1.00
0.49 0.34 1.00
0.49 0.34 1.00
0.49 0.34 1.00
0.27 0.48 1.00
0.27 0.48 1.00
0.27 0.48 1.00
0.12 0.58 1.00
0.12 0.58 1.00
0.12 0.58 1.00
- - -
- - -
- - -
 Calc. Area
Calc. Area iPrOH Sum Check Average Standard C Results for Each Trial
nBuOH

Sample Species 1
92.2 5.9 101.6347593 Trial (#)
Name Area (-)

74.6 22.1 104.91125 A6 1 1.3E+08

50.8 43.8 109.3312225 A6 2 1.4E+08
26.2 66.3 113.9041004 A6 3 1.3E+08
12.7 78.6 116.4105545 Average 1.4E+08
29.5 65.8 115.167257 B6 1 1.3E+08
92.8 5.4 101.5313819 B6 2 1.3E+08
68.5 27.6 106.0432902 B6 3 1.3E+08
52.4 42.3 109.0310858 Average 1.3E+08

29.2 63.6 113.3452439 C6 1 1.3E+08

10.6 80.6 116.8128196 C6 2 1.2E+08
29.7 68.5 117.3053507 C6 3 1.2E+08
75.2 21.6 104.8066606 Average 1.2E+08
63.8 31.9 106.9119917 D6 1 1.3E+08
49.3 45.2 109.6098163 D6 2 1.3E+08
38.2 55.3 111.6727913 D6 3 1.3E+08
25.9 66.6 113.9686196 Average 1.3E+08
29.3 66.7 115.7453974 Overall Average 1.3E+08
92.8 5.4 101.5283202
70.3 26.0 105.7014819
48.2 46.2 109.8134313
27.6 65.0 113.6488921 MeOH
13.7 77.8 116.2388659 30.00
29.0 65.4 114.6013373 25.00
f(x) = 4.85045736120393E-05x + 0.4289440171
20.00 R² = 0.9156739547
Area y (%)

15.00

10.00

5.00

0.00
0.0E+00 1.0E+05 2.0E+05 3.0E+05 4.0E+05 5.0E+0
Concentration (mM)

iPrOH
100.00
90.00 f(x) = 0.0001004524x + 3.4365188358
80.00 R² = 0.9971582843
 iPrOH
100.00
90.00 f(x) = 0.0001004524x + 3.4365188358
80.00 R² = 0.9971582843
70.00
60.00

Area y (%)
50.00
40.00
30.00
20.00
10.00
0.00
0.0E+00 2.0E+05 4.0E+05 6.0E+05 8.0E+05
Concentration (mM)

nBuOH
80.00
70.00
60.00 f(x) = 0.0001621037x - 4.3179092464
R² = 0.9789924079
50.00
Area y (%)

40.00
30.00
20.00
10.00
0.00
0.0E+00 1.0E+05 2.0E+05 3.0E+05 4.0E+05
Concentration (mM)
d C Results for Each Trial

Sum of Species 1 Species 2 Species 3

Species 2 Species 3 Total Area MeOH CONC
Corrected Pct Area Pct Area Pct Area
Area (-) Area (-) (%) (mM)
Area (-) (%) (%) (%)
1.6E+08 2.7E+08 5.7E+08 23.40 28.54 48.07 100.00 2646.20661142
1.7E+08 2.9E+08 5.9E+08 23.39 28.55 48.06 100.00
1.6E+08 2.8E+08 5.7E+08 23.36 28.52 48.13 100.00
1.6E+08 2.8E+08 5.8E+08 23 29 48
1.7E+08 2.9E+08 5.8E+08 21.86 28.86 49.28 100.00
1.7E+08 2.9E+08 5.8E+08 21.83 28.87 49.30 100.00
1.7E+08 2.9E+08 5.8E+08 21.81 28.86 49.33 100.00
1.7E+08 2.9E+08 5.8E+08 22 29 49

1.7E+08 2.8E+08 5.8E+08 22.82 28.66 48.52 100.00

1.5E+08 2.6E+08 5.3E+08 22.81 28.66 48.53 100.00
1.5E+08 2.5E+08 5.3E+08 22.81 28.66 48.53 100.00
1.6E+08 2.6E+08 5.5E+08 23 29 49
1.6E+08 2.7E+08 5.6E+08 23.53 28.56 47.92 100.00
1.6E+08 2.7E+08 5.7E+08 23.50 28.55 47.95 100.00
1.6E+08 2.7E+08 5.6E+08 23.55 28.55 47.90 100.00
1.6E+08 2.7E+08 5.7E+08 24 29 48
1.6E+08 2.7E+08 5.7E+08 23 29 48

MeOH

045736120393E-05x + 0.4289440171
6739547

2.0E+05 3.0E+05 4.0E+05 5.0E+05 6.0E+05

Concentration (mM)

iPrOH

04524x + 3.4365188358
2843
 iPrOH

04524x + 3.4365188358
2843

4.0E+05 6.0E+05 8.0E+05 1.0E+06

Concentration (mM)

nBuOH

1621037x - 4.3179092464
924079

2.0E+05 3.0E+05 4.0E+05 5.0E+05

Concentration (mM)
iPrOH nBuOH
CONC CONC
(mM) (mM)
 Sample Sample Empty Vial Volume Std Vial Weight Volume Std B
Number (-) Designation (-) Weight (g) A (µl) A (g) (µl)

A1 G4A1 2.242 100 2.313 900

A2 G4A2 2.228 300 2.429 700
A3 G4A3 2.247 500 2.627 500
A4 G4A4 2.242 700 2.774 300
A5 G4A5 2.254 900 2.994 100
A6 G4A6 2.221 - - -
B1 G4B1 2.243 100 2.312 900
B2 G4B2 2.217 300 2.458 700
B3 G4B3 2.239 500 2.573 500
B4 G4B4 2.216 700 2.761 300
B5 G4B5 2.247 900 2.857 100
B6 G4B6 2.181 - - -
C1 G4C1 2.231 100 2.545 900
C2 G4C2 2.248 300 2.675 700
C3 G4C3 2.222 500 2.847 500
C4 G4C4 2.239 700 2.994 300
C5 G4C5 2.186 900 3.115 100
C6 G4C6 2.187 - - -
D1 G4D1 2.212 100 2.282 900
D2 G4D2 2.234 300 2.459 700
D3 G4D3 2.203 500 2.588 500
D4 G4D4 2.215 700 2.74 300
D5 G4D5 2.185 900 2.769 100
D6 G4D6 2.226 - - -

Total Mass of Flask (g)

Standard Stock
Solution Empty Container Species 1 Species 2 Species 3 Species 1
(g) MeOH (g) iPrOH (g) nBuOH (g) w/ Container (g)
102.181 - 199.185 210.272
A 108.091 Mass frac 1 Mass frac 2 Mass frac 3
0.34 0.00 0.66
- 195.573 - -
B 119.002 Mass frac 1 Mass frac 2 Mass frac 3
0.00 1.00 0.00
nBuOH
MeOH (g/mol) IPrOH (g/mol)
(g/mol)
32.04 60.1 74.123
 Vial
Vial Weight B Volume Std C Mass Mass
Weight C Mass A (g) Mass B (g) Mass C (g)
(g) (µl) MeOH (g) iPrOH (g)
(g)

2.971 - - 0.071 0.658 - 0.024 0.658

3.01 - - 0.201 0.581 - 0.068 0.581
3.028 - - 0.38 0.401 - 0.129 0.401
2.958 - - 0.532 0.184 - 0.180 0.184
3.083 - - 0.74 0.089 - 0.251 0.089
- 1000 2.993 - - 0.772 0.178 0.224
2.989 - - 0.069 0.677 - 0.023 0.677
2.984 - - 0.241 0.526 - 0.082 0.526
2.949 - - 0.334 0.376 - 0.113 0.376
2.983 - - 0.545 0.222 - 0.185 0.222
2.912 - - 0.61 0.055 - 0.207 0.055
- 1000 2.974 - - 0.793 0.173 0.229
3.478 - - 0.314 0.933 - 0.106 0.933
3.438 - - 0.427 0.763 - 0.145 0.763
3.468 - - 0.625 0.621 - 0.212 0.621
3.466 - - 0.755 0.472 - 0.256 0.472
3.43 - - 0.929 0.315 - 0.315 0.315
- 1000 3.209 - - 1.022 0.233 0.293
2.97 - - 0.07 0.688 - 0.024 0.688
2.992 - - 0.225 0.533 - 0.076 0.533
2.954 - - 0.385 0.366 - 0.131 0.366
2.936 - - 0.525 0.196 - 0.178 0.196
2.847 - - 0.584 0.078 - 0.198 0.078
- 1000 2.99 - - 0.764 0.180 0.218

Species 2 Species 3 Mass VS Volume

w/ Container (g) w/ Container (g) 1
- 307.276 0.9
0.8
0.7
314.575 -
0.6
Mass (g)

0.5
0.4
0.3
0.2
0.1
0
0 100 200 300 400 500 600

Volume (µl)

Standa rd A Standard B
0.3
0.2
0.1
0
0 100 200 300 400 500 600

Volume (µl)

Standa rd A Standard B
 Mass
Mass frac Mass frac Mass frac Sum
nBuOH Mole MeOH Mole iPrOH
MeOH iPrOH nBuOH Check
(g)

0.047 0.03 0.90 0.06 1.00 7.5E-04 1.1E-02

0.133 0.09 0.74 0.17 1.00 2.1E-03 9.7E-03
0.251 0.16 0.51 0.32 1.00 4.0E-03 6.7E-03
0.352 0.25 0.26 0.49 1.00 5.6E-03 3.1E-03
0.489 0.30 0.11 0.59 1.00 7.8E-03 1.5E-03
0.371 0.23 0.29 0.48 1.00 5.5E-03 3.7E-03
0.046 0.03 0.91 0.06 1.00 7.3E-04 1.1E-02
0.159 0.11 0.69 0.21 1.00 2.6E-03 8.8E-03
0.221 0.16 0.53 0.31 1.00 3.5E-03 6.3E-03
0.360 0.24 0.29 0.47 1.00 5.8E-03 3.7E-03
0.403 0.31 0.08 0.61 1.00 6.5E-03 9.2E-04
0.391 0.22 0.29 0.49 1.00 5.4E-03 3.8E-03
0.208 0.09 0.75 0.17 1.00 3.3E-03 1.6E-02
0.282 0.12 0.64 0.24 1.00 4.5E-03 1.3E-02
0.413 0.17 0.50 0.33 1.00 6.6E-03 1.0E-02
0.499 0.21 0.38 0.41 1.00 8.0E-03 7.9E-03
0.614 0.25 0.25 0.49 1.00 9.8E-03 5.2E-03
0.496 0.23 0.29 0.49 1.00 7.3E-03 4.9E-03
0.046 0.03 0.91 0.06 1.00 7.4E-04 1.1E-02
0.149 0.10 0.70 0.20 1.00 2.4E-03 8.9E-03
0.254 0.17 0.49 0.34 1.00 4.1E-03 6.1E-03
0.347 0.25 0.27 0.48 1.00 5.6E-03 3.3E-03
0.386 0.30 0.12 0.58 1.00 6.2E-03 1.3E-03
0.366 0.24 0.29 0.48 1.00 5.6E-03 3.6E-03

ass VS Volume

400 500 600 700 800 900 1000

Volume (µl)

nda rd A Standard B
400 500 600 700 800 900 1000

Volume (µl)

nda rd A Standard B
 Mole frac Mole frac Mole frac MeOH CONC iPrOH CONC
Mole nBuOH Sum Check
MeOH iPrOH nBuOH (mg/L) (mg/L)

6.3E-04 0.06 0.89 0.05 1.00 2.4E+04 6.6E+05

1.8E-03 0.16 0.71 0.13 1.00 6.8E+04 5.8E+05
3.4E-03 0.29 0.47 0.24 1.00 1.3E+05 4.0E+05
4.7E-03 0.42 0.23 0.35 1.00 1.8E+05 1.8E+05
6.6E-03 0.49 0.09 0.41 1.00 2.5E+05 8.9E+04
5.0E-03 0.39 0.26 0.35 1.00 1.8E+05 2.2E+05
6.2E-04 0.06 0.89 0.05 1.00 2.3E+04 6.8E+05
2.1E-03 0.19 0.65 0.16 1.00 8.2E+04 5.3E+05
3.0E-03 0.28 0.49 0.23 1.00 1.1E+05 3.8E+05
4.9E-03 0.40 0.26 0.34 1.00 1.8E+05 2.2E+05
5.4E-03 0.50 0.07 0.42 1.00 2.1E+05 5.5E+04
5.3E-03 0.37 0.26 0.36 1.00 1.7E+05 2.3E+05
2.8E-03 0.15 0.72 0.13 1.00 1.1E+05 9.3E+05
3.8E-03 0.21 0.60 0.18 1.00 1.4E+05 7.6E+05
5.6E-03 0.29 0.46 0.25 1.00 2.1E+05 6.2E+05
6.7E-03 0.35 0.35 0.30 1.00 2.6E+05 4.7E+05
8.3E-03 0.42 0.22 0.35 1.00 3.1E+05 3.2E+05
6.7E-03 0.39 0.26 0.36 1.00 2.3E+05 2.9E+05
6.2E-04 0.06 0.89 0.05 1.00 2.4E+04 6.9E+05
2.0E-03 0.18 0.67 0.15 1.00 7.6E+04 5.3E+05
3.4E-03 0.30 0.45 0.25 1.00 1.3E+05 3.7E+05
4.7E-03 0.41 0.24 0.35 1.00 1.8E+05 2.0E+05
5.2E-03 0.49 0.10 0.41 1.00 2.0E+05 7.8E+04
4.9E-03 0.40 0.26 0.35 1.00 1.8E+05 2.2E+05
 Average
nBuOH CONC MeOH CONC iPrOH CONC nBuOH CONC Sample MeOH
(mg/L) (mM) (mM) (mM) Name CONC
(mg/L)
4.7E+04 6.1E+04 8.9E+05 5.1E+04 ABCD1 4.4E+04
1.3E+05 1.6E+05 7.1E+05 1.3E+05 ABCD2 9.3E+04
2.5E+05 2.9E+05 4.7E+05 2.4E+05 ABCD3 1.5E+05
3.5E+05 4.2E+05 2.3E+05 3.5E+05 ABCD4 2.0E+05
4.9E+05 4.9E+05 9.3E+04 4.1E+05 ABCD5 2.4E+05
3.7E+05 3.9E+05 2.6E+05 3.5E+05
4.6E+04 5.8E+04 8.9E+05 4.9E+04 Me
1.6E+05 1.9E+05 6.5E+05 1.6E+05
30.00
2.2E+05 2.8E+05 4.9E+05 2.3E+05
3.6E+05 4.0E+05 2.6E+05 3.4E+05 25.00
f(x) = 4.85045736120393
4.0E+05 5.0E+05 7.1E+04 4.2E+05 20.00 R² = 0.9156739547
3.9E+05 3.7E+05 2.6E+05 3.6E+05

Area y (%)
15.00
2.1E+05 1.5E+05 7.2E+05 1.3E+05
2.8E+05 2.1E+05 6.0E+05 1.8E+05 10.00
4.1E+05 2.9E+05 4.6E+05 2.5E+05 5.00
5.0E+05 3.5E+05 3.5E+05 3.0E+05
0.00
6.1E+05 4.2E+05 2.2E+05 3.5E+05 0.0E+00 1.0E+05 2.0E+05
5.0E+05 3.9E+05 2.6E+05 3.6E+05 Conce
4.6E+04 5.8E+04 8.9E+05 4.9E+04
1.5E+05 1.8E+05 6.7E+05 1.5E+05
2.5E+05 3.0E+05 4.5E+05 2.5E+05
3.5E+05 4.1E+05 2.4E+05 3.5E+05 iPrO
3.9E+05 4.9E+05 1.0E+05 4.1E+05 100.00
3.7E+05 4.0E+05 2.6E+05 3.5E+05 90.00 f(x) = 0.0001004524x + 3.436
80.00 R² = 0.9971582843
70.00
60.00
Area y (%)

50.00
40.00
30.00
20.00
10.00
0.00
0.0E+001.0E+052.0E+053.0E+054.0E+055
Concent

nBuO
80.00
70.00
60.00 f(x) = 0.0001621037x - 4.3
R² = 0.9789924079
50.00
Area y (%)

40.00
30.00
 80.00
70.00
60.00 f(x) = 0.0001621037x - 4.3
R² = 0.9789924079
50.00

Area y (%)
40.00
30.00
20.00
10.00
0.00
0.0E+00 5.0E+04 1.0E+05 1.5E+05 2.0E+
Concent
 Average Average Average Average Average
iPrOH nBuOH MeOH iPrOH nBuOH Sample
Mass A (g) Mass B (g)
CONC CONC CONC CONC CONC Name (-)
(mg/L) (mg/L) (mM) (mM) (mM)
7.4E+05 8.7E+04 8.3E+04 8.5E+05 7.0E+04 0.131 0.739 A1
6.0E+05 1.8E+05 1.9E+05 6.6E+05 1.6E+05 0.274 0.601 A1
4.4E+05 2.8E+05 2.9E+05 4.7E+05 2.4E+05 0.431 0.441 A1
2.7E+05 3.9E+05 4.0E+05 2.7E+05 3.3E+05 0.589 0.269 Average
1.3E+05 4.7E+05 4.8E+05 1.2E+05 4.0E+05 0.716 0.134 A2
A2
A2
MeOH
Average
0
A3
0 A3
f(x) = 4.85045736120393E-05x + 0.4289440171
0 R² = 0.9156739547 A3
Average
0
A4
0 A4
0 A4
Average
0
0E+00 1.0E+05 2.0E+05 3.0E+05 4.0E+05 5.0E+05 6.0E+05 A5
Concentration (mM) A5
A5
Average
B1
iPrOH B1
B1
f(x) = 0.0001004524x + 3.4365188358 Average
R² = 0.9971582843
B2
B2
B2
Average
B3
B3
B3
+001.0E+052.0E+053.0E+054.0E+055.0E+056.0E+057.0E+058.0E+059.0E+051.0E+06
Average
Concentration (mM)
B4
B4

nBuOH B4
Average
B5
f(x) = 0.0001621037x - 4.3179092464 B5
R² = 0.9789924079
 f(x) = 0.0001621037x - 4.3179092464
R² = 0.9789924079
B5
Average
C1
C1
C1
Average
+00 5.0E+04 1.0E+05 1.5E+05 2.0E+05 2.5E+05 3.0E+05 3.5E+05 4.0E+05 4.5E+05
C2
Concentration (mM)
C2
C2
Average
C3
C3
C3
Average
C4
C4
C4
Average
C5
C5
C5
Average
D1
D1
D1
Average
D2
D2
D2
Average
D3
D3
D3
Average
D4
D4
D4
Average
D5
D5
D5
Average
Species 1 Species 2 Species 3
Pct Area Pct Area Pct Area
(%) (%) (%)

2.57 90.62 6.80

2.57 90.63 6.80
2.57 90.62 6.81
2.57 90.62 6.81
7.53 76.81 15.66
7.52 76.79 15.69
7.51 76.79 15.70
7.52 76.80 15.68
15.86 51.74 32.40
15.89 51.75 32.36
15.85 51.73 32.42
15.87 51.74 32.39
19.25 26.87 53.88
19.24 26.86 53.90
19.18 26.84 53.98
19.22 26.86 53.92
27.61 10.99 61.40
27.70 11.00 61.30
27.66 10.99 61.35
27.65 10.99 61.35
2.61 91.69 5.71
2.60 91.67 5.73
2.61 91.68 5.71
2.61 91.68 5.72
9.15 71.07 19.78
9.14 71.05 19.81
9.13 71.05 19.82
9.14 71.05 19.81
14.31 52.25 33.45
14.29 52.24 33.47
14.33 52.31 33.36
14.31 52.26 33.43
22.64 30.93 46.43
22.67 30.93 46.40

22.65 30.91 46.45

22.65 30.92 46.43
17.57 8.88 73.55
17.54 8.88 73.58
17.46 8.87 73.66
17.52 8.88 73.60
7.84 75.36 16.80
7.84 75.40 16.75
7.84 75.39 16.76
7.84 75.39 16.77
10.41 65.38 24.21
10.43 65.36 24.21
10.43 65.32 24.25
10.42 65.35 24.22
15.48 50.58 33.93
15.54 50.63 33.83
15.51 50.60 33.89
15.51 50.61 33.89
19.97 38.95 41.07
19.99 38.96 41.05
20.00 38.96 41.05
19.99 38.96 41.06
21.02 26.24 52.74
20.92 26.20 52.87
20.93 26.23 52.84
20.96 26.22 52.82
2.61 90.77 6.62
2.60 90.76 6.64
2.61 90.76 6.63
2.61 90.76 6.63
9.26 70.80 19.94
9.26 70.80 19.94
9.25 70.78 19.97
9.26 70.79 19.95
15.14 49.92 34.94
15.10 49.92 34.97
15.14 49.95 34.91
15.13 49.93 34.94
22.10 28.03 49.88
22.10 28.02 49.88
22.09 28.01 49.89
22.10 28.02 49.88
23.08 11.54 65.38
23.10 11.55 65.35
23.01 11.54 65.45
23.06 11.54 65.39