BAM Media M104: MR-VP Broth

January 2001

Bacteriological Analytical Manual

M104
MR-VP Broth

Medium 1

Buffered peptone-water powder

7g
(Difco or BBL)

Glucose 5g

K2HP04 5g

Distilled water 1 liter

Medium 2

Pancreatic digest of casein 3.5 g

Peptic digest of animal tissue 3.5 g
Dextrose 5.0 g
Potassium phosphate 5.0 g
Distilled water 1 liter

Dissolve ingredients in water with gentle heat if necessary. Dispense 10 ml into 16 x 150 mm test tubes
and autoclave 15 min at 118-121°C. Final pH, 6.9 ± 0.2.

Medium 3
Peptone 5.0 g
Glucose 5.0 g
Phosphate buffer 5.0 g
Distilled water 1 liter
Dissolve ingredients in water. Dispense 10 ml into 16 x 150 mm test tubes and autoclave 15 min at
121°C. Final pH, 7.5 ± 0.2.

For Salmonella: Dispense 10 ml into 16 x 150 mm test tubes and autoclave 12-15 min at 121°C.
For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.