2/10/2019 CN106432479A - Method for extracting collagen from cow leather - Google Patents

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Method for extracting collagen from cow leather

Abstract
CN106432479A
The invention relates to a method for extracting collagen from animal cuticle, in particular to a
China
method for extracting collagen from cow leather; the method comprises the steps of 1, cow leather
pretreatment, 2, decoction and 3, re nement; the method for extracting the collagen from the cow
leather is simple in technology and easy to operate, cow leather resources are fully utilized, a single Download PDF Find Prior Art Similar
act in a traditional method for manufacturing leather products is avoided, the yield of the collagen is
signi cantly improved by the method for extracting the collagen from the cow leather, the yield is up Other languages: Chinese
to 75.6%, and according to the method, the yield of the collagen is not limited by raw materials.
Inventor: 谭春萍, 杨远澄, 周贵金, 陆海龙, 杨远广

Classi cations
Worldwide applications

C07K14/78 Connective tissue peptides, e.g. collagen, elastin, laminin, bronectin, vitronectin, 2016 CN
cold insoluble globulin [CIG]

Application CN201611032604.XA events

2016-11-16 Application led by 广西山水牛农业有限责任公司

2016-11-16 Priority to CN201611032604.XA

2017-02-22 Publication of CN106432479A

Info: Patent citations (1), Non-patent citations (3), Legal events,

Similar documents, Priority and Related Applications

External links: Espacenet, Global Dossier, Discuss

Claims (2) Hide Dependent

translated from Chinese

A method for extracting collagen from the leather, characterized by comprising the steps of: (1) Kraft Pretreatment: Fresh shaved leather, meat removed subcutaneous, 0.15 to
0.2% physiological saline soaked leather 25 ~ 30min, rinsed with water leather 10 ~ 15min, the cut leather pieces, degreasing agent added, soak 35 ~ 40min, ltered, the lter
residue was added 3 to 5 times the mass of tartaric acid soak 20 ~ 25min, rinsed with water to a residue solution PH value of 6-7, to obtain leather residues; degreasing agent,
the enzyme is degreased, chloroform, methanol and distilled water of 1: 1.7 to 2.2: 0.5 to 0.8: 5.5 to 6 mass ratio of mixing at 55 to 60 after the step ⑴ residue treated leather
and drying to a moisture content of 5.5 to 7%, added water and stirred for 55 ~ 65min at 100 ° C: (2) co-boil; boil at 45 ~ 55min ° C, to , was cooled to 40 ~ 50 ° C, using heated
water bath, was added a solution of citric acid adjusted PH value of 4.5 to 5.5, temperature was stirred for 2 ~ 3h, ltered off with suction; ⑶ puri cation: the ltrate obtained in
step ⑵ the suction, the ltrate was added mass 60 to 70% ethanol, at 40 ~ 50 ° C water extraction 50 ~ 60min, recovering ethanol by distillation under reduced pressure,
resulting residue was added to the residue Dichloromethane was 4 to 5 times, the thermostat was stirred 90 ~ lOOmin, methylene chloride recovered by distillation under
reduced pressure, water bath temperature was adjusted to 100 ~ 120 ° C, stirring was continued for 10 ~ 15min, to.

2. The method of extracting collagen from cowhide according to claim 1, wherein said degreasing enzyme, a phosphatase, an enzyme sterol, Bacillus subtilis esterase
and a fat hydrolase species.

Description translated from Chinese

A method for extracting collagen from cowhide

FIELD

[0001] The present invention relates to a method for extracting collagen from animal epidermis, particularly relates to a method for extracting collagen from cowhide.

Background technique

[0002] Collagen is an extracellular protein, peptide chains of three twisted helical brous protein, collagen rich in amino acids, essential amino acids accounting for
15.78% of the total amino acids, higher nutritional value of protein, collagen protein products have been widely used in food, health food, cosmetics, pharmaceuticals,
etc., a sharp increase in market demand, and therefore extraction technology of collagen more and more attention; rich in collagen leather, but leather is generally used as
shoes, handbags , belts and other leather goods, in which collagen is broken out in leather processing, resulting in a waste of resources, the current method for extracting
collagen concern are varied, but due to unreasonable extraction method, leading to a lower rate and purity of puri ed collagen, a serious impact on the latter its value, but
also a waste of resources, how to improve the traditional extraction methods on extraction rate and to achieve higher purity collagen is particularly important.

SUMMARY

[0003] The present invention is to solve the above problems, there is provided a method of extracting collagen from cowhide.

[0004] DETAILED is achieved by technical solution:

[0005] - methods of collagen extracted from the leather, comprising the steps of:

[0006] (1) Kraft Pretreatment: Fresh shaved leather, meat removed subcutaneous, 0.15 to 0.2% physiological saline soaked leather 25 ~ 30min, 10 ~ 15min leather rinsed
with water, cut into pieces cowhide adding a degreasing agent, soak 35 ~ 40min, ltered, the lter residue was added 3 to 5 times the mass of tartaric acid soak 20 ~
25min, to rinse the lter cake with an aqueous solution of PH value of 6 to 7 to give leather residue.

[0007] The degreasing agent, the enzyme is degreased, chloroform, methanol and distilled water of 1: 1.7 to 2.2: 0.5 to 0.8: 5.5 to 6 mass mixing ratio, boiled 45 ~ 55min
at 55 ~ 60 ° C, It can be.

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2/10/2019 CN106432479A - Method for extracting collagen from cow leather - Google Patents
[0008] (2) co-boil: the step ⑴ residue treated leather and drying to a moisture content of 5.5 to 7%, water was added, with stirring at 100 ° C for the 55 ~ 65min, cooled to
40 ~ 50 ° C, heating using water bath, was added citric acid Zi PH value of the solution is adjusted to 5.5 4.5, temperature was stirred for 2 ~ 3h, ltered off with suction.

[0009] (3) puri cation: The ltrate obtained by suction ltration step (2) was added to the ltrate 60 to 70 mass% ethanol at 40 ~ 50 ° C water extraction 50 ~ 60min, the
recovery of ethanol by distillation under reduced pressure, resulting residue was added to 4 to 5 times the residual liquid of dichloromethane, stirred thermostat 90 ~
lOOmin, methylene chloride recovered by distillation under reduced pressure, water bath temperature was adjusted to 100 ~ 120 ° C, stirring was continued for 10 ~
15min, to.

[0010] Further, the defatted enzyme, a phosphatase, an enzyme sterol, fat carboxylesterase and one subtilisin hydrolase.

[0011] In conclusion, the bene cial effects of the present invention is that: the process is simple, easy to operate, leather full use of resources, to avoid the conventional
method for the production of leather goods single practice, after the method of the invention signi cantly increase the yield of collagen, up 75.6%, the method of the
present invention is not limited to the material yield of collagen.

Detailed ways

[0012] The following speci c embodiments of the present invention will be further described in detail, but the present invention is not limited to these embodiments, and
any modi cations or embodiments in place of the basic spirit claimed in the present embodiment, still belongs to the claims of the present invention range.

[0013] Example 1

[0014] - methods of collagen extracted from the leather, comprising the steps of:

[0015] (1) Kraft Pretreatment: Fresh shaved leather, skin removed meat, with 0.15% saline soaked cowhide 25min, rinsed with water lOmin leather, the cut leather pieces,
degreasing agent added, soaking 35min, ltered, the lter residue was added three times the mass of tartaric acid soak 20min, rinsed PH value of 6 to aqueous residue
with water to obtain leather residue.

[0016] The degreasing agent, the enzyme is degreased, chloroform, methanol, and distilled water 1: 1.7: 0.5: 5.5 ratio of mass, boiled at 55 ° C 45min, can.

[0017] (2) co-boil: after the step ⑴ kraft process residues, and drying to a moisture content of 5.5%, water was added, stirred 55min at 100 ° C, cooled to 40 ° C, using
heated water bath, was added citric acid solution adjusted PH value of 4.5, the thermostat was stirred 2h, ltered off with suction.

[0018] (3) puri cation: The step (2) in the ltrate obtained by suction ltration, the ltrate was added 60% by mass of ethanol, at 40 ° C for water extraction 50min,
recovering ethanol by distillation under reduced pressure, resulting residue was added to the residue methylene chloride solution four times, the thermostat was stirred
90min, dichloromethane recovered distillation under reduced pressure, water bath temperature was adjusted to 100 ° C, stirring was continued for lOmin, can.

[0019] The degreasing enzyme, a phosphatase.

[0020] Example 2

[0021] - methods of collagen extracted from the leather, comprising the steps of:

[0022] (1) Kraft Pretreatment: Fresh shaved leather, skin removed meat, with 0.2% physiological saline soaked leather 30min, 15min leather rinsed with water, the cut
leather pieces, degreasing agent added, soaking 40min, ltered, the lter residue was added 5 times the mass of tartaric acid soaking 25min, rinsed aqueous residue PH
value to 7 with water to give leather residue.

[0023] The degreasing agent, the enzyme is degreased, chloroform, methanol, and distilled water 1: 2.2: 0.8: 6 mass ratio of mixed, boiled at 60 ° C 55min, can.

[0024] (2) co-boil: after the step ⑴ kraft process residues, and drying to a moisture content of 7%, water was added, stirred 65min at 100 ° C, cooled to 50 ° C, using
heated water bath, was added citric acid solution to adjust the PH value is 5.5, temperature was stirred 3h, ltered off with suction.

[0025] (3) puri cation: The step (2) in the ltrate obtained by suction ltration, the ltrate was added 70% by mass of ethanol, at 50 ° C for water extraction 60min,
recovering ethanol by distillation under reduced pressure, resulting residue was added to the residue methylene chloride solution 5 times, the thermostat was stirred
lOOmin, dichloromethane recovered distillation under reduced pressure, water bath temperature was adjusted to 120 ° C, stirring was continued for 15min, to.

According to [0026] Degreasing enzyme, the enzyme is a sterol.

[0027] Example 3

[0028] - methods of collagen extracted from the leather, comprising the steps of:

[0029] (1) Kraft Pretreatment: Fresh shaved leather, skin removed meat, with 0.18% saline soaked cowhide 28min, 13min leather rinsed with water, the cut leather pieces,
degreasing agent added, soaking 38min, ltered, the lter residue was added 4 times the mass of tartaric 23min soaking, rinsing aqueous residue PH value to 6.5 with
water to give leather residue.

[0030] The degreasing agent, the enzyme is degreased, chloroform, methanol, and distilled water 1: 2: 0.7: 5.8 mass mixing ratio, boiled at 58 ° C 50min, can.

[0031] (2) co-boil: after the step ⑴ kraft process residues, and drying to a moisture content of 6%, with water, stirred for 60min at 100 ° C, cooled to 45 ° C, using heated
water bath, was added citric acid solution adjusted PH value of 5, the thermostat was stirred 2.5h, ltered off with suction.

[0032] (3) puri cation: The step (2) in the ltrate obtained by suction ltration, the ltrate was added 65% by mass of ethanol, at 45 ° C water extraction 55min, recovering
ethanol by distillation under reduced pressure, resulting residue was added to the residue 4.5 times the methylene chloride, the thermostat was stirred 95 min,
dichloromethane recovered distillation under reduced pressure, water bath temperature was adjusted to ll〇 ° C, stirring was continued for 13min, to.

[0033] The degreasing enzyme is carboxylesterase.

[0034] Example 4

[0035] - methods of collagen extracted from the leather, comprising the steps of:

[0036] (1) Kraft Pretreatment: Fresh shaved leather, skin removed meat, with 0.2% physiological saline soaked leather 25min, 15min leather rinsed with water, the cut
leather pieces, degreasing agent added, soaking 35min, ltered, the lter residue was added three times the mass of tartaric acid soaking 25min, rinsed PH value of 6 to
aqueous residue with water to obtain leather residue.

[0037] The degreasing agent, the enzyme is degreased, chloroform, methanol, and distilled water 1: 2.2: 0.5: 5.5 ratio of mass, boiled at 55 ° C 55min, can.

[0038] (2) co-boil: after the step ⑴ kraft process residues, and drying to a moisture content of 7%, water was added, stirred 55min at 100 ° C, cooled to 50 ° C, using
heated water bath, was added citric acid solution to adjust the PH value is 5.5, temperature was stirred 3h, ltered off with suction.

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2/10/2019 CN106432479A - Method for extracting collagen from cow leather - Google Patents
[0039] (3) puri cation: The step (2) in the ltrate obtained by suction ltration, the ltrate was added 60% by mass of ethanol, at 40 ° C for water extraction 60min,
recovering ethanol by distillation under reduced pressure, resulting residue was added to the residue methylene chloride solution four times, the thermostat was stirred
90min, dichloromethane recovered distillation under reduced pressure, water bath temperature was adjusted to 120 ° C, stirring was continued for 15min, to.

[0040] The degreasing enzyme, fat hydrolase is subtilisin.

[0041] Example 5

[0042] - methods of collagen extracted from the leather, comprising the steps of:

[0043] (1) Kraft Pretreatment: Fresh shaved leather, skin removed meat, with 0.2% physiological saline soaked leather 25min, rinsed with water lOmin leather, the cut
leather pieces, degreasing agent added, soaking 35min, ltered, the lter residue was added three times the mass of tartaric acid soaking 25min, rinsed aqueous residue
PH value to 7 with water to give leather residue.

[0044] The degreasing agent, the enzyme is degreased, chloroform, methanol, and distilled water 1: 2.2: 0.5: 6 mass ratio of mixed, boiled at 55 ° C 45min, can.

[0045] (2) co-boil: after the step ⑴ kraft process residues, and drying to a moisture content of 7%, water was added, stirred 55min at 100 ° C, cooled to 40 ° C, using
heated water bath, was added citric acid solution to adjust the PH value is 5.5, temperature was stirred 3h, ltered off with suction.

[0046] ⑶ puri cation: the step (2) in the ltrate obtained by suction ltration, the ltrate was added 70% by mass of ethanol, at 50 ° C for water extraction 50min,
recovering ethanol distilled under reduced pressure to give residual liquid, the residual liquid was added 5 times dichloromethane, was stirred thermostat 90min,
dichloromethane recovered distillation under reduced pressure, water bath temperature was adjusted to 120 ° C, stirring was continued for lOmin, can.

According to [0047] Degreasing enzyme is carboxylesterase.

[0048] Example 6

[0049] The same operating method as in Example 1, except that the dry fresh cowhide leather replacement for the preservation for 3 months.

[0050] Example 7

[0051] Use the same operation as in Example 1, except that the degreasing agent was not added in the step ⑴.

[0052] Example 8

[0053] Use the same operation as in Example 1, except that in the puri cation step to cancel the ⑶ step.

[0054] Experimental Example

[0055] statistics of the embodiment of the present invention the yield of collagen 1 to 8, the results shown in Table 1:

[0056] TABLE 1

[0057]

[0058] Description: Yield collagen Examples 1 to 5 of the present embodiment of the invention, up to 75.6%, Example 6 instead of fresh leather, yield and yield of collagen
leather fresh approaches, the method of the present invention described yield is not limited to collagen raw material, in Example 7 without adding a degreasing agent,
greatly reducing the yield of collagen step without puri cation Example 8 embodiment, to reduce the yield of collagen.

[0059] Examples 6 to 8 are embodiments Example 1 as control, if the embodiment 2 to 5 as a control for comparison, the same conclusions.

Patent Citations (1)

Publication number Priority date Publication date Assignee Title

CN105061588A * 2015-08-27 2015-11-18 长春博泰医药生物技术有限责任公司 Cowhide processing method for preparing collagen

Family To Family Citations

* Cited by examiner, † Cited by third party

Non-Patent Citations (3)

Title

沈菊泉等: "酸法制备牛皮胶原蛋白及其结构性质研究", 《食品工业》 *

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2/10/2019 CN106432479A - Method for extracting collagen from cow leather - Google Patents
王娜等: "皮胶原的结构、性质与提取方法", 《皮革科学与工程》 *

赵博: "枯草杆菌脂肪水解酶LipA 和LipB", 《中国生物化学与分子生物学报》 *

* Cited by examiner, † Cited by third party

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Priority And Related Applications

Priority Applications (1)

Application Priority date Filing date Title

CN201611032604.XA 2016-11-16 2016-11-16 Method for extracting collagen from cow leather

Applications Claiming Priority (1)

Application Filing date Title

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Legal Events

Date Code Title Description

2017-02-22 C06 Publication

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2017-03-22 C10 Entry into substantive examination

2018-01-02 TA01

Concepts

machine-extracted Download Filter table

Name Image Sections Count Query match

Bos taurus abstract,title 6 0

Collagen abstract,title 7 0

Collagen abstract,title 7 0

collagen abstract,title 7 0

collagens abstract,title 7 0

engineering processes abstract 1 0

improved abstract 1 0

leather abstract,title 7 0

products abstract 1 0

raw materials abstract 1 0

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