Protocol 1 p1000

Initials Pipette Protocol Weight measured (g) Weight Expected (g)

EH 1000ul x 1 0.993
EH 200ul x 5 0.987 0.998
HN 1000ul x 1 0.992 0.998
HN 200ul x 5 0.986 0.998
DG 1000ul x 1 1.003 0.998
DG 200ul x 5 0.991 0.998
CK 1000ul x 1 0.966 0.998
CK 200ul x 5 0.922 0.998
HC 1000ul x 1 0.966 0.998
JM 200ul x 5 1.007 0.998
MW 1000ul x 1 0.994 0.998
MW 200ul x 5 0.993 0.998
NM 1000ul x 1 1.002 0.998
NM 200ul x 5 1.007 0.998
MC 1000ul x 1 0.991 0.998
MC 200ul x 5 1.007 0.998
DIG 1000ul x 1 0.996 0.998
DIG 200ul x 5 1.013 0.998
1000ul x 1 1.001 0.998
MB-T 200ul x 5 1.002 0.998
MB-T 1000ul x 1 1.001 0.998
200ul x 5 0.998
CH 1000ul x 1 0.975 0.998
CH 200ul x 5 0.995 0.998
JG 1000ul x 1 1.002 0.998
JG 200ul x 5 1.001 0.998
MZ 1000ul x 1 0.987 0.998
MZ 200ul x 5 0.989 0.998
PS 1000 ul x 1 0.992 0.998
PS 200ul x 5 1.011 0.998
CC 1000ul x 1 1.001 0.998
CC 200ul x 5 0.99 0.998
AR 1000 ul x 1 0.994 0.998
AR 200ul x 5 1.006 0.998
Protocol 2 p200 Protocol 3
Pipette Protocol Weight measured (g) Weight Expected (g) Pipette Protocol
200ul x 1 0.201 0.199 10ul x 2
50ul x 4 0.203 0.199 5ul x 4
200ul x 1 0.202 0.199 10ul x 2
50ul x 4 0.203 0.199 5ul x 4
200ul x 1 0.202 0.199 10ul x 2
50ul x 4 0.208 0.199 5ul x 4
200ul x 1 0.2 0.199 10ul x 2
50ul x 4 0.203 0.199 5ul x 4
200ul x 1 0.206 0.199 10ul x 2
50ul x 4 0.197 0.199 5ul x 4
200ul x 1 0.202 0.199 10ul x 2
50ul x 4 0.198 0.199 5ul x 4
200ul x 1 0.204 0.199 10ul x 2
50ul x 4 0.197 0.199 5ul x 4
200ul x 1 0.201 0.199 10ul x 2
50ul x 4 0.212 0.199 5ul x 4
200ul x 1 0.2 0.199 10ul x 2
50ul x 4 0.199 0.199 5ul x 4
200ul x 1 0.201 0.199 10ul x 2
50ul x 4 0.201 0.199 5ul x 4
200ul x 1 0.205 0.199 10ul x 2
50ul x 4 0.205 0.199 5ul x 4
200ul x 1 0.2 0.199 10ul x 2
50ul x 4 0.199 0.199 5ul x 4
200ul x 1 0.201 0.199 10ul x 2
50ul x 4 0.205 0.199 5ul x 4
200ul x 1 0.199 0.199 10ul x 2
50ul x 4 0.199 0.199 5ul x 4
200ul x 1 0.2 0.199 10ul x 2
50ul x 4 0.198 0.199 5ul x 4
200ul x 1 0.201 0.199 10ul x 2
50ul x 4 0.197 0.199 5ul x 4
200ul x 1 0.202 0.199 10ul x 2
50ul x 4 0.201 0.199 5ul x 4
p10 Protocol 4 Serological Pipette
Weight measured (g) Weight Expected (g) Pipette Protocol Weight measured (g)
0.019 0.019 1000ul x 2 1000ul x 2
0.017 0.019 200ul x 6 200ul x 6
0.019 0.019 5ml x 1 5.066
0.018 0.019 2.5ml x 2 5.081
0.016 0.019 5ml x 1 4.94
0.015 0.019 2.5ml x 2 4.9
0.017 0.019 5ml x 1 4.93
0.022 0.019 2.5ml x 2 4.91
0.016 0.019 5ml x 1 5.021
0.02 0.019 2.5ml x 2 5.03
0.019 0.019 5ml x 1 4.95
0.018 0.019 2.5ml x 2 4.941
0.019 0.019 5ml x 1 5.03
0.02 0.019 2.5ml x 2 5.03
0.018 0.019 5ml x 1 5.014
0.019 0.019 2.5ml x 2 4.994
0.019 0.019 5ml x 1 4.912
0.019 0.019 2.5ml x 2 4.769
0.016 0.019 5ml x 1
0.016 0.019 2.5ml x 2 4.289
0.017 0.019 5ml x 1 4.238
0.019 2.5ml x 2
0.015 0.019 5ml x 1 5.052
0.017 0.019 2.5ml x 2 5.002
0.016 0.019 5ml x 1 4.99
0.017 0.019 2.5ml x 2 5.01
0.017 0.019 5ml x 1 5.019
0.019 0.019 2.5ml x 2 4.968
0.017 0.019 5ml x 1 5.032
0.021 0.019 2.5ml x 2 4.934
0.019 0.019 5ml x 1 4.92
0.017 0.019 2.5ml x 2 4.848
0.021 0.019 5ml x 1 5.02
0.018 0.019 2.5ml x 2 4.927
 Protocol 5 Pipetting Oil
Weight Expected (g) Pipette Protocol Weight measured (g)Weight Expected (g)
1.003 1.008 200ul x 2 0.197
1.009 1.02 50ul x 5 0.195
4.99 1000ul x 1 0.851 0.93
4.99 200ul x 1 0.171 0.186
4.99 1000ul x 1 0.82 0.93
4.99 200ul x 1 0.17 0.186
4.99 1000ul x 1 0.79 0.93
4.99 200ul x 1 0.18 0.186
4.99 1000ul x 1 0.854 0.93
4.99 200ul x 1 0.17 0.186
4.99 1000ul x 1 0.903 0.93
4.99 200ul x 1 0.172 0.186
4.99 1000ul x 1 0.857 0.93
4.99 200ul x 1 0.186
4.99 1000ul x 1 0.893 0.93
4.99 200ul x 1 0.157 0.186
4.99 1000ul x 1 0.71 0.93
4.99 200ul x 1 0.126 0.186
4.99 1000ul x 1 0.93
4.99 200ul x 1 0.824 0.186
4.99 1000ul x 1 0.135 0.93
4.99 200ul x 1 0.186
4.99 1000ul x 1 0.9 0.93
4.99 200ul x 1 0.179 0.186
4.99 1000ul x 1 0.824 0.93
4.99 200ul x 1 0.135 0.186
4.99 1000ul x 1 0.841 0.93
4.99 200ul x 1 0.162 0.186
4.99 1000ul x 1 0.823 0.93
4.99 200ul x 1 0.128 0.186
4.99 1000ul x 1 0.725 0.93
4.99 200ul x 1 0.175 0.186
4.99 1000ul x 1 0.888 0.93
4.99 200ul x 1 0.182 0.186
 Mean (g) SD (g) (Mean +/- SD) (g)
p1000 0.9928 0.0168 1.0097
0.9760
p200 0.2015 0.0032 0.2048
0.1983
p10 0.0180 0.0017 0.0197
0.0162
Serological 4.9256 0.1923 5.1179
4.7332
Expected Value (g) (Meaured-Expected) (g) Accuracy (%) Precision (%)
0.9980 -0.0052 0.5192 1.6960

0.1990 0.0025 1.2711 1.6032

0.0190 -0.0010 5.4226 9.6873

4.9900 -0.0644 1.2912 3.9049