JOURNAL OF EXPERIMENT

Title of Experiment : Urine

Day/Date of Experiment : Monday/ 30 September 2019
Name : Suci Indah Sari
ID : 1713440007
Class/Group : ICP of Chemistry Education / V
Group Members : 1. Nurleni
2. Yuliani Susilomaris
3. Radiatul Awalia Amir
4. Nurul Hikmah
Assistant : Risdah Damayanti N. S,Pd

A. OBJECTIVES
1. Know how to identification organic and inorganis substances which contain in
urine.
2. Know how to differentiate between normal and abnormal urine
B. APPARATUS AND CHEMICALS
1. Apparatus
a. Test tubes (16 units)
b. Rack of test tube (3 units)
c. Small hose (1 unit)
d. Stirrer (2 units)
e. Regular funnel (1 unit)
f. Drops pipette (10 units)
g. Measure glass 10 mL and 25 mL @(2 units)
h. Beaker glass 500 mL (1 unit)
i. Erlenmeyer flask 100 mL (1 unit)
j. Wood clamp (2 units)
k. Spray bottle (1 unit)
l. Bunsen burner (2 units)
m. Tripod and gauze @(1 unit)
n. Watch glass (1 unit)
o. Spatula (2 units)
p. Stopwatch (1 unit)
q. Rough and soft cloth @(1 unit)
2. Chemicals
a. Urine
b. Silver nitrate dilute solution (AgNO3)
c. Ammonium molybdat solution ((NH4)2Mo7O24)
d. Nitric acid concentrate solution (HNO3)
e. Acid chloride solution 1 M (HCl)
f. Barium chloride solution 0.1 M (BaCl2)
g. Sodium hydroxide solution 0.1 M, 1 M, 2.5 N (NaOH)
h. Barium hydroxide solution (Ba(OH)2)
i. Ammonium oxalate saturate solution ((NH4)2C2O4)
j. Acetic acid solution 0.1 M, 2 N and concentrate (CH3COOH)
k. Acetic acid glacial solution (CH3COOH)
l. Ammonium hydroxide solution 1 M (NH4OH)
m. Xanhydrol in methanol solution
n. Sodium nitroprusid solution 0.1 M and 5% (Na2[Fe(CN)5NO])
o. Ammonium sulphate solid (NH4)2SO4)
p. Glucose solution 1% (C6H12O6)
q. Urea solution 0.1 M (CO(NH2)2)
r. Red phenol solution 0.04% (C6H5OH)
s. Soybean powder
t. Oxalic acid solution 0.1 M (H2C2O4)
u. Copper sulphate solution 0.01 M (CuSO4)
v. Aquadest (H2O)
w. Nessler reagent
x. Benedict reagent
y. Fehling reagent
z. Tollens reagent
aa. Cotton
bb. Aluminium foil
cc. Matches
dd. Litmus paper
ee. Spiritus
ff. Tissue
gg. Filter paper
C. WORK PROCEDURES
1. Determination the organic substances of urine
a. Cl-

3 mL of urine 5 drops of AgNO3 dilute Observe

b. PO43-

3 mL of urine 1 mL of Several drops of Observe

(NH4)2Mo7O24 HNO3 concentrate
c. SO42-

3 mL of urine 3 drops 3 drops Filter Save the filtrate

of BaCl2 0.1 M of HCl 0.1 M
d. NH4+

3 mL of urine 3 drops of NaOH 0.1 M Divide into 2 parts

Part 2 of Nessler Part 1 of Ba(OH)2

solution reagent solution solution

e. Ca2+

5 mL of urine Several drops Several drops of Obeserve

of (NH4)2C2O4 saturate CH3COOH concentrate
f. Mg2+

10 mL of urine Several drops Several drops Several drops

of NaOH 0.1 M of CH3COOH of (NH4)2C2O4 saturate
until base until acid until form precipitate
 Close with cotton Add the filtrate Filter
if there is no precipitate of NH4OH

g. Test of Xanhydrole Urea

1 mL of urine 500 mL of H2O Take 5 mL 5 mL of CH3COOH

Wait for several times Close and shake 0.5 mL of xanhydrole

until dixanhydrolurea separate in methanol

h. Test of Nitroprusid Creatinine

5 mL of urine 5 drops of sodium Drops by drops Heat it

Nitroprusid 0.1 M of NaOH 1 M
until form red color
 Heat it again Add CH3COOH glacial
during 1 minute
i. Test of ketones

10 mL of urine Saturating with 2-3 drops 1-2 mL of NH4OH

(NH4)2SO4 solid of sodium concentrate
Nitroprusid 5%

Wait for several minutes Mix

during 30 minutes
j. Test of reducing sugars

5 drops of urine 5 mL of 5 mL of 5 mL of
Benedict’s Fehling’s Tollens
reagent reagent reagent
(Tube 1) (Tube 2) (Tube 3)
 Repeat for
glucose 1%

Heat for several times

k. Test of Protein Coagulation

5 mL of urine Heat for 1-2 minutes 3-5 drops Observe

of CH3COOH 2 N

Several drops of
CH3COOH until excessive
l. Dissociation react of Urea by Urease

3 mL of 4 drops of Drop by drops 0.5 gram

urea 0.1 M red phenol 0.04% of NaOH 0.1 M of soybean
until form pink solution powder
 Until form a change Shake
m. Formation of urea-oxalate

Repeat
for Urine
1-2 drops 1 drop of Stir it
of urea 0.1 N H2C2O4 0.1 M

n. Formation of Biuret

1 gram of urea Melt it Observe its smell Heat it until freeze

Drop by drop of 1 mL of NaOH 2.5 N Shake 2 mL of H2O Cool it

CuSO4 0.01 M
until form color change
Makassar, September 24th 2019
Assistant, Practican,

Risdah Damayanti N. S,Pd Suci Indah Sari

ID. 1713440007