Given:

Absorbance of sample: 0.954

Absorbance of standard: 1.034
Concentration of standard: 49.1 g/L
Unknown:
Let X = Concentration of sample

Computation:
(Absorbance of sample/Absorbance of standard) x Concentration of standard = Concentration of sample
(0.954/1.034) x 49.1 g/L = X
(0.9226305609) x 49.1 = X
X = 45.30116054 g/L ~ 45.30 g/L

The concentration of the albumin in the sample was 45.30 g/L and it was within the normal range
for adults ( 35 - 50 g/L).

CONCLUSION

Albumin is known for its ability to bind many types of organic compounds, including organic
dyes. When albumin binds with Bromcresol Green (BCG) it causes a change in the absorbance
maximum of BCG. This change can be measured spectrophotometrically and used to determine
albumin concentration. Hypoalbuminemia is the decrease in albumin which is caused by a
decrease in synthesis secondary to liver diseases, reduced absorption of amino acids due to
malnutrition. Hyperalbuminemia is of little diagnostic significance except in dehydration. No
clinical conditions are known that cause the liver to produce excess albumin. Another clinical
application of albumin concentration is that it affects the plasma protein binding of drugs. The
clinical importance of plasma protein binding is to help the interpretation of measured drug
concentrations. Albumin levels are low in many disease states, resulting in lower total drug
concentrations.