DOCUMENTATION

1. Preparation Of Eluen Solution ( Motion Phase )

Tools in this experiment Materials in this experiment

Entered 25 mL n-butanol + 6 mL
glacial acetic acid + 25 mL aquadest
into chromatography cabinet :
colorless solution

2. Determination The Component of Amino Acid

Made a distance 1 cm from bottom of Oven for 5 minutes : white
and 0,5 cm from the top and made 4
spot which labeled A, B,C and S

Dropped 4 solution ( A, B, C and S) in Hung up in the chromatography

each spot 3 times cabinet that contain of eluent

After the eluen reach the upper limit of Dried in oven for 5 minutes
chromatography paper

Sprayed with ninhydrin Dried in oven for 5 minutes again

After dried 5 minutes the color on the Coat with tape of the entire of
papet appear : chromatography paper
A : brownish
B : orange (+)
C : orange
S : orange (+)