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  • C-92 IC Application Note No.: Title: Amines in Fish

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    Ramiro Pulgarín
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    This document summarizes a study that used cation chromatography with direct conductivity detection to determine levels of 6 amines (dimethylamine, trimethylaminoxide, trimethylamine, putrescine, cadaverine, and histamine) in a sample of fresh fish (plaice). The fish sample was homogenized, centrifuged, and filtered before injection onto a Metrosep C 2 column with 6.0 mmol/L nitric acid mobile phase. The results provide the mean concentration of each amine detected over 5 injections of the fish sample.

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    This document summarizes a study that used cation chromatography with direct conductivity detection to determine levels of 6 amines (dimethylamine, trimethylaminoxide, trimethylamine, putrescine, cadaverine, and histamine) in a sample of fresh fish (plaice). The fish sample was homogenized, centrifuged, and filtered before injection onto a Metrosep C 2 column with 6.0 mmol/L nitric acid mobile phase. The results provide the mean concentration of each amine detected over 5 injections of the fish sample.

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    C-92 IC Application Note No.: Title: Amines in Fish

    Uploaded by

    Ramiro Pulgarín
    This document summarizes a study that used cation chromatography with direct conductivity detection to determine levels of 6 amines (dimethylamine, trimethylaminoxide, trimethylamine, putrescine, cadaverine, and histamine) in a sample of fresh fish (plaice). The fish sample was homogenized, centrifuged, and filtered before injection onto a Metrosep C 2 column with 6.0 mmol/L nitric acid mobile phase. The results provide the mean concentration of each amine detected over 5 injections of the fish sample.

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    © All Rights Reserved
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    IC Application Note No.

    C-92
    Title: Amines in Fish

    Summary: Determination of dimethylamine (DMA), trimethyl-


    aminoxide (TMAO), trimethylamine (TMA), putrescine,
    cadaverine and histamine in a fish sample using cation
    chromatography with direct conductivity detection.

    Sample: Fish (fresh plaice)


    Sample Preparation: 5 g sample homogenized in 50 mL of 0.1 mol/L acetate
    buffer (pH = 4.8), centrifuge and filter (0.45 µm) prior to
    injection

    Column: 6.1010.220 Metrosep C 2 – 150


    Eluent: 6.0 mmol/L nitric acid
    Flow: 1.0 mL/min
    Injection Volume: 20 µL

    mV

    400

    300
    TMA

    200
    Cadaverine
    Putrescine
    DMA

    Histamine
    TMAO

    100

    ch1
    0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 min

    Results: DMA-N TMAO-N TMA-N Putrescine-N Cadaverine-N Histamine-N


    mg/kg mg/kg mg/kg mg/kg mg/kg mg/kg
    Mean of 5 injections 10.37 3.78 129.70 7.16 5.39 n.d.

    C-92

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