Solution: Sample Conc. Response

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solution

Sample Conc. Response


5 46
10 102
15 155
350
20 205

response
25 250 300
30 298 f(x) =
250 R² =
Y = a.x + b
200
slope 10.02286
intercept 0.60000 150
corr. Coeff. 0.998460370 rsq
100
Estimated dilute concentration
50
Reading Linear Interpolation x = (y – b) / a undiluted concentration
55 5.4462 5.4276 1089.24 0
60 5.4339 5.9265 1086.78 0 5
100 9.1084 9.9173 1821.68
220 21.6678 21.8900 4333.56
250 25.0000 24.8831 5000.00

units aliquot amount taken diluted to


g 5 100
ml 1 10

dilution factor 200

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solution

Calibration line

350
response

300
f(x) = 10.0228571429x + 0.6
250 R² = 0.9984603701

200

150

100

50

0
0 5 10 15 20 25 30 35
concentration

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