Regent strip test Principle Reagents Color reaction Sources of

interference

pKa change of M: Poly(methylvinyl F(+): High

1. Specific gravity polyelectrolytes ether) maleic Blue-green-yellow concentration of
anhydride, BTB protein
(45 s) F(–): Highly alkaline
C: Ethyleneglycol- urine
bis
(aminoethylether),
BTB

Double indicator Methyl red; Red-yellow-blue Ru nover from

2. pH system bromthymol blue adjacent pads Old
(60 s) specimens

Protein error of M: F(+): Highly buffered

3. Protein indicator Tetrabromphenol Blue-green alkaline urine,
blue pigmented specimens,
chlorhexidine,
(60 s) phenazopyridine,
C:Tetrachloropheno QACs (detergents),
l tetrabromosulfon- antiseptics, loss of
phthalein buffer, high SG
F(–):Proteins other
than albumin, high salt
concentration,
microalbuminuria

Glucose oxidase M: Glucose F(+): Oxidizing

4. Glucose reaction oxidase, M: green-brown agents, detergents
peroxidase, KI C: yellow-green F(–): Ascorbic acid,
ketones, high SG,
C: Glucose oxidase, (30 s) low temperatures,
peroxidase, TMB improperly
preserved
specimens

Sodium M: Sodium F(+): Phthalein dyes,

. Ketones nitroprusside nitroprusside Purple highly pigmented red
reaction urine, levodopa,
medications
C: Sodium (40 s) containing SH group
nitroprusside and F(–): Improperly
glycine preserved specimens

Pseudo-peroxidase M: F(+): Strong oxidizing

6. Blood activity of heme Diisopropylbenzene Blue-green agents, bacterial
dihydroperoxide peroxidases,
menstrual
TMB (60 s) contamination
F(–): High SG,
C: 2,5-dimethyl 2,5- crenated cells,
dihydroperoxyhexan formalin, captopril,
e TMB nitrite, ascorbic acid,
unmixed specimen
 Diazo reaction M: 2,4- F(+): Highly
7. Bilirubin dichloroaniline Tan, pink, or violet pigmented urine,
diazonium salt indican,
(30 s) phenazopyridine,
C:2,6- metabolites of
dichlorobenzene Lodine
diazoniumtetrafluorob F(–): Specimen
orate exposure to light,
ascorbic acid >25
mg/dL, high
concentration of
nitrite

Ehrlich reaction M: p-diethylamino- F(+): PBG, indican,

8.Urobilinogen benzaldehyde Red procaine, p-
aminosalicylic acid,
C: 4- (60 s) sulfonamides,
methoxybenzene methyldopa,
diazonium chlorpromazine,
tetrafluoro-borate pigmented urine
F(–): Old
specimens,
formalin, high
concentration of
nitrite

Greiss reaction M: p-arsanilic acid, F(+): Old specimen,

9. Nitrite tetrahydrobenzoquin Pink highly pigmented
olinol urine
(60 s) F(–):Nonreductase-
C: sulfanilamide, 3- containing bacteria,
hydroxy- 1,2,3,4- lack of urinary
tetrahydro- 7,8- nitrate, insufficient
benzoquinoline contact time
between bacteria
and nitrate, bacteria
converting nitrite to
nitrogen, antibiotics,
ascorbic acid, high
SG

Granulocytic M: Derivatized F(+): Strong

10. Leukocyte esterase reaction pyerole amino acid Purple oxidizing agents,
esterase ester, diazonium formalin, highly
salt (120 s) pigmented urine,
nitrofurantoin
C: Indoxylcarbonic F(–): protein,
acid ester, glucose, oxalic acid,
diazonium salt ascorbic acid,
gentamicin,
cephalosporins,
tetracyclines