RESULTS AND DISCUSSION

Table 1. Mortality or EC 50 of dead Daphnia per treatment in the 2 Sources of Effluent

Treatments Total Number of dead Total Number of dead

Daphnia Daphnia

(Source 1) (Source 2)

T0- (as positive control with 0 3

pure distilled water)
T1 (100%) - (80 ml 11 (EC50) 10(EC50)
wastewater
+ 0 ml distilled water)
T2 (50%) – (40 ml 5 5
wastewater
+ 40 ml distilled water)
T3 (25%) – (20 ml 2 3
wastewater
+ 60 ml distilled water)
Total 18 21

Table 1 shows the mortality or EC 50 of dead Daphnia per treatment in Source

1 (Purok 7) and source 2. T0, T2 and T3 in both of the sources did not establish an

EC50 . An acceptable EC50 test will have at least two test concentrations where the

number of immobile (dead) animals bracket 50 percent unless there is less than 50

percent response in the 100 percent solution at the solubility limit of the toxicant in

water.

T1 in both of the sources established an EC50 because above 50% of the

daphnids subjected in this treatment died.

It was observed also that as the concentration of wastewater increases, the

death of the Daphnia magna increases, too. Therefore, the two variables are directly

related.
Table 2. One- Way Analysis of Variance on the Mortality of Daphnia magna in

Source 1

Source of Degrees of Sum of Mean Computed Tabular F

Variation Freedom Squares Square F 5% 1%

Between Groups 2 0.5 0.2500 0.1401 4.26 8.02

(Treatment)

Within Groups 9 16.0625 1.7847

(Experimental Error)

Total 11 16.5625

Decision= not significant at 5% and 1 % level

Table 2 shows the One- Way Analysis of Variance on the Mortality Rate of

Daphnia in Source 2.

Based on the result of statistical analysis even though there are slight

differences with the mortality means of Daphnia magna applied with different

treatments with varying concentrations of effluent, there is no significant difference

between and among them.

Sources of error might include the intervening variables like the dissolved

oxygen and others.

Temperature and the pH of the solution are not the source of experimental

error because the two parameters were monitored in the time of the experiment.

To obtain a better data, the addition of replicates per treatment should be done

in future research and also if possible, reduce the value of the experimental error.
Table 3. One- Way Analysis of Variance on the Mortality Rate of Daphnia in Source 2

Source of Degrees of Sum of Mean Computed Tabular F

Variation Freedom Squares Square F 5% 1%

Between Groups 2 .6668 0.3334 0.2308 4.26 8.02

(Treatment)

Within Groups 9 13 1.4444

(Experimental Error)

Total 11 13.6668

Decision= not significant at 5% and 1 % level

Table 3 shows the One- Way Analysis of Variance on the Mortality Rate of

Daphnia in Source 2.

Based on the result of statistical analysis even though there are slight

differences with the mortality rate means of Daphnia Magna applied with different

treatments with varying concentrations of effluent, there is no significant difference

among them.

Sources of error might include the intervening variables like the dissolved

oxygen and others.

Temperature and the pH of the solution are not the source of experimental

error because the two parameters were monitored in the time of the experiment.

To obtain a better data, the addition of replicates per treatment should be done

in future research and also if possible, reduce the value of the experimental error
Table 4. Mortality of Daphnia magna in Source 1 (Purok 7)

Treatment O R1 R2 Total Mean

T0- (as positive control with pure 0 0 0 0 0

distilled water)
T1 (100%) - (80 ml wastewater 4 4 3 11 3.66
+ 0 ml distilled water)
T2 (50%) – (40 ml wastewater 2 1 2 5 1.66
+ 40 ml distilled water)
T3 (25%) – (20 ml wastewater 1 0 1 2 .66
+ 60 ml distilled water)
Total= 18

Table 4 shows that the effluent from source 1 affects the mortality of Daphnia

magna applied with Treatment 1, 2, and 3 which might have been caused by the toxic

contaminants found in the effluent. It might have been a good result because there are

no dead daphnia in Treatment 0. Treatment 1 has the highest total of dead daphnia

which is 11 with 80 ml wastewater and 0 ml distilled water solution. Treatment 2 and

treatment 3 has a total of 5 and 2 dead Daphnia, respectively.

It is worth pointing out that the mean value of the mortality in Treatment 1 is

the highest among all the treatments. Daphnids in Treatment 1 were expected to be

severely affected because of greater quantity of toxic contaminants in the effluent.

During the collection of effluents from the creek in Purok 7, it was observed that

bubbles were found on the flowing wastewater, foul odor was smelled, and weeds

were growing on the soil near it. Therefore, contaminants like phosphates (due to the

occurrence of the bubbles) might be found in the wastewater where the Daphnia were

subjected and caused the death of the test organisms.

Table 5. Mortality of Daphnia magna in Source 2 (Purok 3)

Treatment O R1 R2 Total Mean

T0- (as positive control with pure 1 1 1 3 1

distilled water)
T1 (100%) - (80 ml wastewater 2 5 3 10 3.33
+ 0 ml distilled water)
T2 (50%) – (40 ml wastewater 2 2 1 5 1.67
+ 40 ml distilled water)
T3 (25%) – (20 ml wastewater 1 0 2 3 1
+ 60 ml distilled water)
Total= 21

Table 5 shows that the effluent from source 2 affects the mortality rate of

Daphnia Magna applied with Treatment 1, 2, and 3 which might have been caused by

the toxic contaminants found in the effluent. Treatment 1 has the highest total of dead

daphnia which is 10 with 80 ml wastewater and 0 ml distilled water solution.

Treatment 2 and treatment 3 has a total of 5 and 3 dead daphnia, respectively.

It is worth pointing out that the mean value of the mortality rate in Treatment

1 is the highest among all the treatments. Daphnids in Treatment 3 were expected to

be severely affected because of greater quantity of toxic contaminants in the effluent.

During the collection of effluent sample, it was observed that the creek in Purok 3 was

narrow, wastewater coming from the residents was freely flowing, little pieces

garbages were found, and the wastewater has a slightly brown color. Contaminants

like oil and grease (due to the occurrence of brown color) might be found in the

wastewater where the Daphnia were subjected to.

 Lowest total of dead Dapnia was obtained in T3 and in the control. This is

because of the idea that smaller quantity of toxic contaminants was introduced to the

test organisms in these treatments.

Table 6: Total number of dead Daphnia in Source 1 and Source 2

Source Total Dead Daphnia

1 (Purok 7) 18

2 (Purok 3) 21

Table 6 shows the total number of Daphnia in Source 1 and Source 2. Higher

total of mortality was obtained in source 2 with a total of 21 dead Daphnia. Lower

total of mortality was obtained in source 2 with a total of 21 dead Daphnia.

With these results, the researchers may infer that most of the residents in

Purok 3 produce more pollutants that may cause pollution to the creeks that flows

going to the bodies of water. Furthermore, these results should be presented to the

barangay officials for them to implement rules and regulations to mitigate water

pollution and lessen the extinction of living fauna in the bodies of water.