SUMMARY, CONCLUSION AND RECOMMENDATIONS

Summary

The experiment conducted a bioassay test to observe the effects of the effluent from

the selected creeks of Pinsao Pilot Project, Baguio City with the idea that the death of the test

organisms could mean the occurrence of toxic contaminants in the effluent.

Based on the results, the following are the salient findings from this research

1. T0, T2 and T3 of Sources 1 and 2 did not establish an EC50 . T1 in both of the

sources established an EC50 because above 50% of the daphnids subjected in this treatment

died.

2. Based on the result of statistical analysis even though there are slight differences

with the mortality means of Daphnia magna applied with different treatments with varying

concentrations of effluent, there is no significant difference between and among them.

3. Treatment 1 in both of the sources has the highest total of dead daphnia which is 18

and 21 for Source 1 and 2, respectively. It is worth pointing out that the mean value of the

mortality in Treatment 1 is the highest among all the treatments. Daphnids in Treatment 1

were expected to be severely affected because of greater quantity of toxic contaminants in the

effluent.

4. Higher total of mortality was obtained in source 2 with a total of 21 dead Daphnia

and lower mortality was obtained in source 1 with a total of 18 dead daphnids.
Conclusions

Based on the results, the following conclusions were drawn:

1. When the concentration of wastewater was increased, the mortality of Daphnia

increases, too.

2. There are no significant differences between and among the treatments.

3. Treatment 1 in both sources have the highest total of dead Daphnia.

4. Higher total of mortality was obtained in source 2 with a total of 21 dead Daphnia

and lower mortality was obtained in source 1 with a total of 18 dead daphnids.

5. Wastewater coming from the selected creeks of Pinsao can’t severely affect the test

organisms since results prove that EC50 was not obtained in all of the treatments.

Recommendations

Based on the findings, the following recommendations were drawn:

1. Since EC50 was established in one of the treatments, this research must be

presented to the residents of Pinsao Pilot Project and the barangay officials as well for them

to be aware about the quality of water in the creeks and for them to find ways to lessen water

pollution in the community.

2. Addition of replicates in each treatment is recommended for future studies to obtain

better results.

3. Another study using other test substance can be conducted for future research.

4. Another study using other test organisms can be conducted.

5. Other parameters like dissolved oxygen can be measured for quality research.
 6. Other endpoints like increased heart rate and decreased appetite can be investigated

for further research aside from mortality.