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Genetics, Lecture 12 (LEcture Notes)
Genetics, Lecture 12 (LEcture Notes)
Nabeel
MOHAMMAD Basheer
AL- RUSAN
Prokaryotic Gene10
1
8/11/2010
Expression
2
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What I want to talk today about it, is the regulation of gene
expression in prokaryotes, We already talked about the gene
regulation and expression in eukaryotes, talking about the
different transcription factors and how they bind to the
promoter, response elements, enhancers, etc. and we talked
about the leucine zipper and the zinc fingers transcription
factors and how their structure regulates transcriptions.
What I am going to talk about today is the regulation of gene
expression in prokaryotes and I'll talk briefly about one
system.
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into glucose and galactose. Now we will look at the anatomy of
this Operon.
And the Lac repressor, protein that is produced from this Lac I
regulatory sequence will bind to the operator.
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Both promoter and operator have specific regulatory sequence
,that means each has a specificity to their own proteins.
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We can summarize the action of the inducer of the Lac
Operon in five steps:
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without these factors the binding affinity for the promoter to
RNA polymerase is very low, so RNA polymerase cannot form a
stable complex with the promoter.
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for the cell to metabolize lactose for energy because it will
use glucose as the source of energy, it's favorable.
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So we have negative control and positive control in the
presence and absence of inducers and the presence and
absence of glucose, depends on the physiological condition of
the cell in the presence or the absence of the cAMP, glucose
or lactose, the Lac Operon will be regulated under these
different conditions.
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