Table 1

Test for Carbohydrates Expected Observation for a Substance indicated by a

Positive Test Positive Test

Iodine solution Purple - black coloration is None

observed in a positive
result.

Molisch's An appearance of reddish Potato isolate & unkown

violet or purple colored ring solution
at the junction of 2 liquids is
observed in a positive
Molisch Test

Benedict's Orange coloration Glucose and Fructose

indicates a positive result

Seliwanoff's A cherry red color is Fructose

observed in a positive
reaction

Bial's orcinol The formation of a bluish None

product indicates positive
result.

Barfoed's A scanty brick red Fructose

precipitate will be
observed in a positive
reaction.
 Table 2. Qualitative Test Result for Carbohydrates

Carbohydrate Visible Test Result

Solution
Benedict's Test Barfoed's Test Seliwanoff's Bial's Test
Test

Glucose Orange with Light blue color Clear / no Light orange

blue color (+) (*) color (-) color (*)

Fructose Turbid Orange Light blue with Cherry red Dark orange
(+) red precipitate color (+) color (*)
(*)

Galactose dark blue Light blue color Clear / no Orange color

green color (*) (*) color (-) (*)

Lactose Light blue with Light blue color Turbid white to Yellow color (-)
Dark green (-) Clear (-)
color (*)

Starch Light blue color Turbid light Turbid white to Yellow orange
(-) blue (-) Clear / no color (-)
color (-)

Liver Dark blue color Turbid light Turbid white Turbid orange
hydrolysate with dark blue blue (*) color (*) (*)
green turning
to brown (*)

Cassava / Dark blue (*) Light blue color Turbid white Orange color
Potato (*) color (*) (*)
hydrolysate

Unknown Dark blue Light blue color Clear / no Orange color

green with (*) color (*) (*)
brown color (*)

In the table above, the results from the performed qualitative tests were shown.
The positive sign (+) indicates that a positive result is shown on the performed experiment
while the negative sign (-) indicates a negative result. The asterisk (*) indicates
questionable or inaccurate result.
 Description Molisch's Test KI/12 (Iodine
solution)

Isolate I (Liver Light brown color is Appearance of Turbid white color

hydrolysate) observed violet or purple appeared
colored ring at the
junction of 2 liquids
is observed

Isolate II (Potato Semi turbid with Appearance of Formation of Light

hydrolysate) Brown color violet or purple Orange color
colored ring at the appeared
junction of 2 liquids
is observed

Unknown Clear and No color Appearance of Clear or No color

violet or purple
colored ring at the
junction of 2 liquids
is observed

Hydrolysate Description (Viscosity) Benedict's Test

Acid I (Liver hydrolysate) High viscosity Formation of dark blue

color

Acid II (Potato hydrolysate) Low viscosity Formation of dark blue

color

Unkown solution Low viscosity Presence of Dark Blue

Green with Brown color
Seliwanoff & Benedicts Testhttps://www.slideshare.net/katealyssacaton/seliwanoff-benedicts-test
Reference: (S.S.R Mauritius) Qualitative tests for carbohydrates
https://www.slideshare.net/namarta28/qualitative-tests-for-carbohydrates-3588