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Biologic interfaces in esthetic dentistry. Part I: the perio/restorative interface

Article  in  European journal of esthetic dentistry : official journal of the European Academy of Esthetic Dentistry, The · June 2011
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scientific session

Biologic Interfaces in Esthetic


Dentistry. Part I: The Perio/
Restorative Interface*
**Gerd Körner, Dr med dent
Educational board member of the German Society of Implantology (DGI),
Dresden International University (DIU) and Ernst-Moritz-Arndt University Greifswald
Member of AAP, EAO, DGP, NEUE Gruppe, DGI, DGÄZ and DGZMK
Private dental practice, Bielefeld, Germany

**Arndt Happe, Dr med dent, DDS


Educational board member of the German Associations for Esthetic Dentistry (DGÄZ),
Periodontology (DGP) and Implantology (DGI)
Private Practice, Münster, Germany

* This article is Part I of a two-part review on biological interfaces in esthetic dentistry that took place
at the European Association of Esthetic Dentistry (EAED) Active Members Meeting in October 2010
in Tremezzo, Italy. Please see Part II (Eur J Esthet Dent 2011;6:226–251) for discussion of the peri-
implant/restorative interface.

** Both authors contributed equally to this article.

Correspondence to: Gerd Körner


Private dental practice, Bielefeld, Germany; phone: +49-521-17-9688; fax: +49-521-17-9855;

e-mail: gerd.koerner@paroplant.com; web: www.paroplant.com

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The scientific chairman’s formation, along with outstanding clin­ical

prologue skills and the contemporary advances


of technology and dental materials can
by Prof. Aris Petros Tripodakis conduct the realization of the ultimate
biologic response of an esthetic out-
In witnessing esthetics within the human come. However a clear documentation,
body, the issues of beauty, health, and definition, and demonstration of the limits
integrity work in continuous synergy. through an interdisciplinary official com-
The pursuit of dental esthetics should munication, is still academically missing.
embrace equally all three in serving the One of the major scopes of the EAED
patient as a person. During the clinical is “to provide leadership in the profes-
practice of esthetic dentistry, the critical sion by defining the highest ethical
issues that differentiate an excellent end standards and to foster interdisciplinary
result from a failure lie mostly within the communication and research through
crucial areas where these items come publications and educational presenta-
into contact: the interfaces. tions.” It is for this reason that the 2010
Biologic and material interfaces in re- Active Members’ Meeting has been
storing the mutilated dentition can com- structured as a workshop aiming to give
pose a clinical intervention of harmony. a documented clinical interpretation of
But which are the attainable limits of clini- the existing scientific evidence con-
cal achievements? Evidence-based in- cerning specific biologic interfaces in

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­esthetic dentistry. The meeting aims to shape and size of teeth to improve the
be a thorough and well-structured dis- smile, which in turn should fit perfectly
cussion that will generate conclusions. in the general esthetic appearance of
For this reason the discussion will be the patient. However when there is the
founded on presented essays address- need to use prosthetic therapy, espe-
ing specific issues divided in two parts cially in periodontally susceptible pa-
as follows: tients, many questions arise.
„„Part I: the perio/restorative interface Surgical and prosthetic crown length
„„Part II: the peri-implant /restorative modifications, surgical and orthodontic
interface. leveling of gingival margins, improving
quality and quantity of attached gingiva,
At this point, it is more than appropri- periodontal plastic surgery procedures
ate for the Scientific Chairman of this such as root coverage, ridge augmen-
meeting to extend genuine gratitude to tation, recapture of papilla height are all
the two essayists, Dr Gerd Körner and problems which the clinician is confront-
Dr Arndt Happe and to the two mod- ed with, every day. Hard and soft tis-
erators, Dr Giano Ricci and Prof. Jörg sues need to be mastered in the proper
Strub for their willingness to invest their way. Stability of the gingival margins,
hard work and time toward the success absence of pocket depth, functionality
of such a demanding workshop. Also, and esthetic long-term results are key-
a warm invitation is extended to all the words for the sophisticated operator.
Life and Active Members of the Acad- Location of the prosthetic finish line
emy to respond to this opportunity by is a fundamental aspect of the esthetic
actively participating in the workshop, outcome. A good long-term result will
as proposed in the guidelines. depend on many different considera-
tions, such as tooth position, periodon-
tal biotype, susceptibility to periodontal
Moderator’s introduction disease, control of inflammation, and
good oral physiotherapy. In order to
by Dr Giano Ricci maintain marginal soft tissue stability, all
of these parameters must be kept under
The times when people wanted just to control along with proper prosthetic ma-
save the natural dentition without con- nipulation. It is imperative not to violate
cern for esthetics have long gone. To- the biologic width. The influence of the
day’s periodontal treatment requires type of restorative material, provided it
great consideration for good esthetic is precise, smooth, polished and doesn’t
results at the end of therapy. In most allow plaque accumulation, has yet to
cases, to completely solve the function- be scientifically demonstrated.
al and esthetic problems of periodontal In patients who have undergone peri-
disease or periodontal therapy, there is odontal surgery for treatment of the at-
the need to utilize a combined surgical tachment apparatus, for periodontal
and prosthetic approach, ­especially plastic surgery or for crown lengthen-
when it is necessary to recapture the ing procedures, it is mandatory to wait

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a long period of time, much longer than ural tooth surface.” This statement by
usually believed especially in esthetic Wunderlich and Cafesse (1985)1 will
cases. This precaution is fundamental stay true even in the future. Ideally no
in order to allow proper maturation of the restoration should approach the gingival
tissues, which present a wide range of apparatus. However, as case demands
patient-related responses. often dictate violating this ideal, the in-
The most demanding esthetic chal- tention of this article is to clarify different
lenge is the recapture of papilla height factors influencing the relationships be-
both around natural teeth or implants. tween the periodontal surroundings and
Different surgical techniques have the restorative situation on the interface.
been proposed but the results seem
questionable and very technically sen-
sitive. It is in this area that surgical and Essay 1: Esthetics and
prosthetic procedures may be really
stability of the marginal
complementary and must act together
interface as influenced
to obtain the ultimate result. This will
be accomplished and maintained in
by its location and the
the long term only if the oral hygiene of restorative material
the patient is excellent and a strict pro-
tocol of supportive periodontal therapy 1.1 The periodontal soft tissue
is followed. The tooth is secured in the alveolar
The planned discussion regarding bone by a combination of connective
most of the above issues will be found- tissue and epithelial attachment.2 Con-
ed on presented essays addressing the nective tissue attaches to a tooth in two
specific items as follows: distinct areas: below the alveolar crest
„„esthetics and stability of the marginal and above the alveolar crest. With this,
interface as influenced by its loca- maxillary gingival fiber bundles pro-
tion and the restorative material vide additional attachment to secure
„„long-term stability of marginal surgi- the tooth in the alveolus, but they also
cal intervention: crown lengthening, serve to immobilize the gingival tis-
guided tissue regeneration and soft sues in relation to the supra-alveolar
tissue grafting portion of the root cementum. This tis-
„„predictability and long-term stability sue immobility, along with resistance
of reconstructing the interproximal to bacterial and mechanical challeng-
papilla in abutment teeth and in pon- es, contributes to the maintenance of
tic areas. a permucosal seal. The outer part of
this seal is constituted by three types
of epithelium.
The essays The junctional epithelium attaches
to the tooth and occupies the area be-
“There is no doubt that at present no tween the most coronal attachment of
man-made restorative can match the the supra-­alveolar connective tissue and
biologic acceptance of a hygienic nat- the base of the gingival crevice. The thin,

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non-keratinized sulcular epithelium lines a clin­ical observation7 regarding only


the entire gingival sulcus and provides maxillary central incisors, a “linear figure
the first line of defence against bacter­ of 3  mm” was reported, determined by
ial ingress or toxin penetration into the probing through the sulcus to the bone
underlying tissue. The oral epithelium crest. However, this dimension varied
lines the external gingival surface from depending on whether it was measured
the crest of the free gingiva to the muco­ at the interproximal papilla or the mid-
gingival junction.3 This relatively thick facial aspect.
keratinized epithelium which is firmly at- In a recent study, Perez et al8 conclud-
tached to the underlying connective tis- ed that variations exist within patients for
sue provides resistance to the forces of similar and different tooth types, arches,
mastication and oral hygiene. and surfaces. Sclar9 summarizes that
nevertheless, although the exact dimen-
1.2 The biologic width sion of biologic width for a particular
clinical situation cannot be determined
The term “biologic width” derives from to date, the concepts derived from these
histometric measurements of some studies can serve as important guide-
of the above-mentioned structures. lines in the clinical restorative and surgi-
In 1961, Gargiulo, Wentz and Orban4 cal practice.
conducted a landmark study of the di-
mension of the dental gingival junction 1.3 Location of the margin
in humans, and re-evaluated and add-
ed to the original data by Orban and Following Maynard and Wilson,10 a dis-
Kohler in 1924.5 The biologic width is tance of 0.5 to 1.0  mm between the re-
defined as the combined dimensions storative margin and the base of the sul-
of the supra-alveolar connective tissue cus is generally considered to be safe.
attachment and junctional epithelial at- To ensure an esthetic and ­physiologic
tachment with a mean value of 2.04  mm. intra-crevicular restoration, they sug-
It represents a dimension of 1.07  mm gested a minimum depth of 1.5 to 2.0  mm
for connect­ive tissue attachment and from the free gingival margin to the base
0.97  mm for epithelial attachment, the of the sulcus prior to intra-sulcular margin
mean of raw data with a very large range. preparation. Furthermore the claim has
Additionally, the tooth type and the di- been made that approximately 5  mm of
mension of the sulcus were not consid- keratinized gingiva, composed of 2  mm
ered. Few studies6–7 have offered more of free gingiva and 3  mm of attached
information on the magnitude of the gingiva, is necessary to maintain health
supra-­alveolar tissues. Basically they when the margins of the restor­ations are
confirmed the above-mentioned data extended into the sulcus.
and found a mean value of 1.34  mm for The placement of restoration margins
sulcus depth, but revealed dissimilari- subgingivally is generally discriminated
ties between different tooth types, with as an invasion of the biologic width,9 and
molars at statistically significantly high- may not only create a direct operative
er mean values than anterior teeth. In trauma to the tissues11 but may also fa-

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cilitate subgingival plaque accumulation Considering that there has been an in-
with resultant inflammatory alterations in creasing demand for esthetic restor­ations
the adjacent gingiva.12–15 All investiga- in recent years, the type of restorative
tions are supporting the landmark study material and the subgingival microflora
by Valderhaug 198016 regarding the features after the placement of well-fin-
negative impact of subgingival restor­ ished subgingival ­restorations were of
ation margins followed by 40% showing interest for Paolantonio et al.15 In a short-
supragingival exposure already after term clinical and microbiological investi-
1 year. At the 10-year examination, as gation over a 1-year observation period,
many as 71% of the restorations had be- amalgam, glass-ionomer cement, and
come supragingivally positioned due to composite resin subgingival restorations
unesthetic recession of the soft tissue did not significantly effect the clinical pa-
margin. rameters recorded. However, composite
Stetler and Bissada17 could demon- resin restorations may have some nega-
strate that teeth with subgingival resto- tive effects on the quantity and the quality
ration margins and a narrow (<  2  mm) of the subgingival plaque. Compared to
band of keratinized gingiva in the apico–­ other investigations26-29 the effects were
coronal direction showed more pro- not as detrimental to gingival health. The
nounced clinical signs of inflammation dissimilarity was explained by the small
than restored teeth with a wide gingival number of subjects highly motivated
zone. But there was no difference in loss towards oral hygiene and by the accu-
of probing attachment. However, if sub- rate contouring, finishing, and polishing
gingivally placed restorations facilitate of subgingival restorations.30-31 These
plaque accumulation and the adjacent findings are supported by a review from
so-called “gingival biotype”18–19 is “thin- Quirynen and Bollen32 explaining the in-
scalloped,” there may be a potential risk fluence of surface roughness and sur-
for the development of soft tissue reces- face-free energy on supra- and subgin-
sion. This conclusion can be drawn from gival plaque formation in man.
findings in an animal model20 and from Rough surfaces will promote plaque
clinical observation.21 formation and maturation, ­high-energy
surfaces are known to collect more
1.4 Influence of material plaque. Although both variables in-
teract with each other, the influence of
Subgingival restoration margins ­neither surface roughness overrules that of the
prevent recurrence of decay,22–23 nor surface free energy. In accordance to
do they stop the onset of gingivitis, peri- these findings, different investigations
odontal attachment loss, or gingival re- are ranking several materials in respect
cession.23-24 Nevertheless there is a ten- to plaque accumulation and biocompat-
dency to hide them, in the sulcus or even ibility:33-39,122
subgingivally, out of esthetic and func- „„glass-ceramics
tional reasons.25 In those situations, den- „„zirconium oxide
tal restoration materials are coming into „„titanium
intimate contact with the adjacent tissues. „„dental porcelain

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Fig 1.1  Situation after periodontal treatment, Fig 1.2   Minimally invasive preparation after ad-
­before restorative correction. hesive filling of cervical erosions with composite.

„„metal alloys Essay 2: Effectiveness


„„composite resin.
and long-term stability
of marginal surgical
1.5 Clinical interpretation
intervention
„„Prefer crevicular or supragingival
margins by minimally to non-invasive
2.1 Surgical crown lengthening
techniques and adhesive ceramics The surgical crown lengthening proce-
(Figs 1.1 and 1.2). dure is often necessary to provide ad-
„„If subgingival margins are inevitable, equate retention and resistance form by
then choose the best ­biocompatible gaining supra-crestal tooth length40 as
materials with optimal biologic re- well as to prevent impingement of res-
sponse with regard to plaque accu- toration margins on the attachment ap-
mulation, to ensure esthetic outcome paratus by re-establishing a logical bio-
and stability in the long run (Figs 1.3 logic width,41–42 especially in cases of
and 1.4). Make sure that the overflow increased esthetic demands. Therefore
of fixation materials can be reached crown lengthening involves the surgical
and properly detached. removal of hard and soft periodontal tis-
„„Go for gingival augmentation to sues.
stabilize the marginal interface for There are only a few controlled stud-
changing the periodontal biotype7 ies43–46 and interestingly they all report
and the dimension of the keratinized that crown lengthening is possible but
gingiva.17 the desired amount of crown lengthen-
„„Downshift the biologic width by ing is either not predictably attained or
resective techniques (surgical crown is subject to change over time. The data
lengthening) to avoid violation of the suggests that there is a significant tissue
biologic width. rebound that has not fully stabilized by

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Fig 1.3   Glass ceramic veneers for best biologic Fig 1.4  Clinical situation after cementation of
response and esthetic outcome. ven­eers 12 to 22.

6 months. This pattern of coronal dis- The best research data available is for
placement of the gingival margin was the coronally advanced flap (CAF) pro-
more pronounced in patients with “thick cedure. It is based on the coronal shift of
gingival biotype” and also appeared to the soft tissues on the exposed root sur-
be influenced by individual variations in face.52-53 This approach may be used
the healing response, not related to gen- alone or in combination with soft tissue
der or age. grafts,54 barrier membranes (BM),55
enamel matrix derivative (EMD),56 acel-
2.2 Root coverage lular dermal matrix (ADM),57 platelet
plasma (RPP),58 and living tissue-engi-
The treatment of gingival recession in neered human fibroblast-derived der-
the area of the perio-restorative inter- mal substitute (HF- DDS).59
face is a common requirement due to Cairo et al60 conducted a systemat-
patient–centered concerns including ic review where only randomized-con-
root sensitivity, difficulty in plaque con- trolled clinical trials (RCTs), including a
trol, increased potential for root caries, split-mouth model of at least 6 months
restorative failure, and compromised es- duration, were considered to measure
thetics.47 and compare clinically relevant out-
The ultimate goal of a root coverage comes for Miller Class I or II localized
procedure is the complete coverage gingival recession defects. Determining
of the recession defect with stable and complete root coverage (CRC) as the
good appearance related to adjacent primary outcome variable revealed the
soft tissues and minimal probing depth most decisive result: only two combina-
(PD).48-50 tions (CAF + CTG and CAF + EMD) pro-
A large variety of different procedures vided better results than CAF alone. And
may be indicated51-52 even though for no other therapy showed better results
some, very limited data are available. than CAF + CTG.

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The decision on whether to select CTG


or EMD in conjunction with CAF could be
influenced by the positive impact of suf-
ficient keratinized tissue (KT)17 following
restorative treatment. This systematic re-
view showed that CAF + CTG were as-
sociated with the best clinical outcome
in terms of KT gain among the compared
combinations. On the other side, CAF +
EMD appears to be an easier procedure
than CAF + CTG and does not require
Fig 2.1  Initial perio-compromised situation.
a donor area for CTG harvest.52 But the
unfavorable cost-benefit ratio of CAF +
EMD, the suboptimal keratinization, and
missing data should be evaluated.
Also in recent years ADM was pro-
posed as a promising alternative to
CTG,61,66 but long-term data are rare or
rather disappointing. Harris62 reported
a study to evaluate the short-term and
long-term coverage results obtained
with ADM and CAF + CTG. He conclud-
ed that the mean results with CAF + CTG
stood the test of time better than ADM.
On the other hand the long-term
stability of the “gold-standard proce-
Fig 2.2   Periodontal regeneration with Emdogain
dure”63 CAF + CTG could be demon-
(EMD) and papilla preservation technique (Cortellini
1995).
strated by Dorfman et al 198264 in a
classic split-mouth study over 4 years.
Different authors65,66 confirmed similar
positive results in favor of grafted sites
with respect to KT, attached gingiva,
and recession. Recent long-term stud-
ies by Agudio et al67,68 with follow-ups
(10–27 years) underlined a sustained
stability of the gingival margin follow-
ing gingival augmentation surgery. The
­contralateral untreated sites showed
a tendency for apical displacement of
the gingival margin with an increase
Fig 2.3   Root coverage of situation Fig 2.1 with an
in the existing recessions. Findings by
autologous connective tissue graft.
Wennström and ­Lindhe69,70 in dog stud-
ies, which concluded that “soft tissue

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grafting was found to be an effective


and predictable means to increase the
width of keratinized tissue but did not
otherwise improve the condition of the
periodontium,” could not be verified.

2.3 Clinical interpretation

„„Surgical crown lengthening and root


coverage are effective treatment mo-
dalities,43–46, 60 and long-term stabil-
ity is reported for the “gold standard Fig 2.4   After regeneration and root coverage with
procedure” – coronally advanced connective tissue, implant placement at tooth 12.
flap in combination with autologous
connective tissue grafts – and with
restrictions the use of EMD63–70
(Figs  2.1–2.6).
„„The impact of biotype and tissue
maturation on the effect of surgi-
cal crown lengthening needs to be
taken into account. Both parameters,
accorded as thick biotypes and less
than 6 months after surgery, can
lead to a marked rebound of tissues
and encroaching of restorative mar-
gins7 (Figs 2.7–2.9).
Fig 2.5  Clinical situation 5 years after restoration:
„„Raising flaps for crown lengthening,
full ceramic crown on implant 12, ceramic veneers
or coronally advancing the pedicle of teeth 13, 11, and 21 to 23.
flap during root coverage via vertical
incisions, is more often now replaced
by incision-less procedures includ-
ing micro-surgical tunnel-techniques
and new suturing methods.119,120
The aim of these up-and-coming
procedures is to minimise trauma to
tissues, avoiding scarring, and re-
ducing mortality.121
„„The question of whether eroded
areas of the denuded root should
be restored before root coverage or
after a sufficient period of healing
cannot be answered definitively be- Fig 2.6   Radiograph situation 5 years after initial
cause of missing data.123 But clinical situation.

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evidence advocates an approach


considering connective tissue as
natural filler at least for moderate
cervical eroded defects.

Essay 3: Predictability
and long-term stability of
reconstructing the inter-
a
proximal papilla next to
restorative situations
Fig 2.7  Situation after conventional periodontal
treatment with persisting violation of biologic width
caused by the old unesthetic restoration. 3.1 Preserving and reconstructing
the interproximal papilla
The interproximal papilla first described
by Cohen in 195971 is the gingival por-
tion, which occupies the space between
two adjacent teeth or adequate clinical
restorations supported by natural teeth
and implants or pontic designs.
The interproximal papilla, playing a
critical role for esthetics and phonet-
ics, may accordingly appear in different
constellations. In any case the founda-
a tion for the structured support is the un-
derlying contour of the osseous crest.
Fig 2.8  Final preparation 6 months after surgery
(maxillary front left).
However the mere existence of the bio-
logic width4,6 with more or less constant
value of 2 mm supra-crestal gingival tis-
sue fails to explain by itself the 5  mm
height72,73 of the interdental papilla.
It became obvious that other key
factors, besides the bone level, may
be involved in the papillary presence/­
absence like the presence of the adja-
cent tooth attachment and the volume
of the gingival embrasure.74,75 Follow-
ing Tarnow et al,73 the vertical height
a
from the base of the interproximal
Fig 2.9  Final situation 1 year later. contact to the bone crest is one deter-
mining factor in maintaining a papilla.
But there are other factors in a more

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Table 1 Tissue height needed from the contact point to the crestal bone level in order to maintain papillae
in different clinical situations. From Zetu and Wang.78

Contact point 100% papillae appearance


Author
alveolar bone crest (mm)

Interdental papillae ≤5 Tarnow et al (1992)

< 4.5 Kois (2001)

Salama et al (1998, 2002),


Implant–tooth papillae < 4.5
Salama (2001)

Implant–implant < 3.5 Tarnow et al (2003)

Implant–pontic < 5.5 Salama et al (2004)

Tooth–pontic < 6.5 Salama et al (2004)

Pontic–pontic <6 Salama et al (2004)

three-dimensional direction like form the needed dimensions among the pos-
and volume of the embrasure, size, sible abutment constellations (Table 1).
shape of the contact area, lateral bone The proper soft tissue management
dimension, root proximity, and biotype is directed to recreate the papillae.97-99
playing a major role.76,77 Hence, there Different techniques for papilla pres-
are different options for therapeutic im- ervation100–105 have been described
pact:78 for beneficial impact on papilla recon-
„„preserving and reconstructing the struction. Especially in regard to implant
interproximal bony support uncovering techniques in combination
„„proper soft tissue management with optional soft tissue grafting, a large
„„beneficial selection of abutment con- variety of techniques were introduced
stellations recently.106–110
„„restorative interproximal design. The predictability of all these tech-
niques remains to be determined.
Socket preservation techniques79-91 Since soft tissue collapse can occur
have been developed to preserve or re- following bone resorption, additional
construct the interproximal space even steps can be taken for the impact on
in combination with forced orthodontic interprox­imal tissue height. Immediate
extrusions92 while maintaining or re- tooth replacement using an ovate pon-
building the soft tissue surroundings. tic to support the papilla for a natural-
Different bone augmentation tech- appearing emergence profile,111 for
niques have been advocated for sup- example in combination with strategic
porting the papilla appearance includ- abutment selection (Table 1) can be an
ing but not limited to GBR,93 onlay advantage. Last but not least, there are
grafting,94 distraction osteogenesis95 different options for having synergetic
and combinations of soft and hard tis- impacts in terms of backing and rein-
sue grafting.96 The aim of reconstruct- forcing the interproximal papilla by a
ing the bony support is to match ideally longer term provisional restoration,112

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Fig 3.1   Periodontally compromised tooth 21 can Fig 3.2   Vertical correction of the defect via osseo­
not be preserved. distraction.

Figs 3.3 and 3.4  Implant 21 uncovering in combination with root coverage of tooth 11 and 22 interprox­
imally and labially at implant site by a connective tissue graft, before and after suturing.

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Fig 3.5  Final restoration: veneers of tooth 11 and Fig 3.6  Final restorative situation 1 year postop-
22 and zirconia-based ceramic restoration of im- eratively.
plant 21.

the interproximal restorative reshap- procedures and/or bone augmenta-


ing,113 and ceramic veneering for al- tion including bone grafting or distrac-
tering the interproximal space. tion osteogenesis. Additional soft tissue
management for tuning the final outline
3.2 Clinical interpretation should be considered (Figs 3.1–3.6).
Synergistic effects can be obtained
The three-dimensional structure of the by strategic selection of abutment con-
interproximal papilla is influenced by stellations, interproximal restorative de-
numerous factors and different constel- sign, and orthodontic impacts.
lations. Provisionalisation is a key element for
Reconstructing a missing papilla is conditioning the interproximal papilla.
a prestigious and bench-marking goal The thereby created individual emer-
of modern treatment concepts. But gence profile should be cautiously trans-
predictability is low and efforts in that ferred into the final restorative situation.
field should be first focused on preserv- Long-term stability has to be seen
ing and then reconstructing. Therefore against this background. Only a few
promising approaches deriving from re- ­articles114-118 have reported a long-term
cent studies of papilla preservation are increase of papilla height, at least around
available.99–104 single implants, even in compromised
In reconstructing this area, the bony situations. But it is evident that there is
support should be enhanced by GTR not sufficient scientific data.

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