Professional Documents
Culture Documents
Final Output. 1111
Final Output. 1111
The Department of Health (DOH) has recorded over 138,000 cases of dengue
fever nationwide in the first three quarters of the year, which represents a 21 percent
increase compared to the number of cases in the same period last year (De Vera, 2018).
Dengue fever is potentially fatal and mainly affects children. Among the hundreds who
died, for instance, the age gap group with the highest case fatality rate is composed of
children who are less than one year old (Geronimo, 2016).
The attraction of mosquitoes towards human beings is due to the lactic acid and
CO2 which is present in our sweat. The chemoreceptors present in the antennae of
mosquitoes perceive the smell of the sweat. The role of natural mosquito repellent is to
mask human scent. Controlling mosquitoes is of utmost importance in the present day
scenario with rising number of mosquito borne diseases. An alarming increase in the
water. Thus, special products like mosquito repellents for combating mosquitoes are
The products used for mosquito repellency show varying degrees of effectiveness.
A number of natural mosquito repellents were studied in many research papers and
review papers that work to repel mosquitoes. Mosquito repellents based on chemicals has
a design with remarkable safety profile, but even then they are toxic against the skin and
nervous system and usually cause rashes, swelling and sometimes eye irritation (Shukla,
2018).
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Insect repellents are important public health tools for the prevention of vector
borne infectious disease (Goddard, 2012). Actions to reduce vector-borne diseases can
result in major health gains and relieve important constraint on development in poor
regions (Amerasinghe, 2010). And this insect repellents help to prevent and control the
outbreaks of insect borne diseases such as malaria, Lyme disease, dengue fever, and
natural that causes insects or other arthropods to make direct oriented movements away
mosquito coil can effectively kill mosquitoes but it has a chemicals that is not safe when
it is expose to people. "Not many people know about it, but the damage done to your
lungs by one mosquito coil is equivalent to the damage done by 100 cigarettes” (Salvi,
2011).
extract as suitable components of the organic mosquito coil. It does not contain harmful
chemicals which are present in some commercial products. Citronella is natural oil found
The researchers decided to make a natural mosquito repellent with the main
ingredients of lemongrass and garlic that has maybe no harmful effects and effectively
repel mosquitoes. To produce a mosquito coil repellent that may repel mosquitoes and
that is affordable and cheaper than other commercial mosquito coil products.
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Statement of the Problem
The main purpose of the study was to determine the effect of alternative mosquito
set-ups?
Hypothesis
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Theoretical Framework
This was anchored in Diane Wells Theory (2017) in which lemongrass is an herb
that can also be used in repelling biting insects such as mosquitoes. Lemongrass oil is
extracted from the lemongrass plant and. In this form, it is easier to use it as a repellent
and lemongrass is a natural repellent because it has high geraniol and citral content. It has
a strong, earthy, fresh, and lemony smell, which repels and biting insects as well.
possible mosquito repellent and have been demonstrated good efficacy against Aedes
spp., and Culex spp. Essential oils of an appreciable number of plants have been shown to
used natural repellents in the world. Lemongrass essential oil is obtained from the aerial
parts of the plant. The plant has been widely recognized for its enthnobotanical and
2017).
These theories are related to our study as it states that the lemongrass and garlic
Conceptual Framework
making alternative mosquito coil repellent. The independent variable were the three set-
ups; Set-up A (3ml lemongrass & 5ml garlic extract); Set-up B (5ml lemongrass & 3ml
garlic extract); Set-up C (5ml lemongrass & 5ml garlic extract). Cassava starch and
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candle wax are also used to mold the mosquito coil repellent. It was an assumption that
the number of mosquito repelled was due to the different concentration of Lemongrass
Figure 1. A Schematic Diagram illustrating the lemongrass and garlic mosquito coil
repellent and the number of mosquito repelled.
This study focused on mosquito coil repellent made from lemongrass and garlic
extract. The lemongrass leaves were taken in Brgy. Taculing. The cassava starch and
candle wax as a mosquito coil binder were bought at Central Market Bacolod. The garlic
used was bought in sari-sari store in Sharina Heights. This study was conducted at
Sharina Heights, Brgy. Taculing, Bacolod City. The researchers used three mosquito nets
to house the 20 mosquitos per net and were tested in two trials for ten minutes per trial.
The same number of mosquitos was tested on the second trial. The experiment was set on
friendly. Production does not involve any use of hazardous substances that could alter our
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environment. Making this alternative mosquito repellent will help the community by its
harmless effect during inhalation of non-toxic substances and its healthier that way.
Homes. Homes are the major beneficiaries of this study. For this alternative
mosquito repellent coil is safe and effective to use. This is the simplest and easiest way to
protect the homes from mosquitoes. This can also be healthy in our homes because of its
natural substances.
alternative mosquito repellent instead of chemical based can be considered as part of the
solution in the mosquito problems. Students also can focus on their studies at night
repellent coil. This is also good for the health of the students because of the natural
Researchers. The positive results of this study would make them helpful
and relatively safe mosquito repellent coil. This can also be safe to the persons who have
Future researchers. This study can help other researchers on the alternative ways
in repelling mosquitoes. This would improve their strategic way of doing their research
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Definition of Terms
To ensure clarity and understanding of the terms in this study, the following terms
Garlic. It refers to a plant in the onion family that’s grown for its distinctive taste
and health benefits (Leech, 2018). In this study, it refers as a one of the main ingredients
that is to be extracted in making the natural mosquito coil repellent and it is the
and Ceylon that is grown in tropical regions for its lemon-scented foliage used as a
seasoning and that is the source of an aromatic essential oil (Merriam, 2019). In this
study, lemongrass refers as one of the main ingredients to be extracted in making the
Mosquito. It refers to a family of flies called the “Culicidae” that can transmit
diseases through its bite (Reinagel, 2010). In this study, mosquito serves as the main
subject.
(Karen, 2018). In this study, mosquito coil is shaped as a spiral and burns slowly in order
mankind (Oxford, 2019). In this study, natural is unmade from humankind, it grows
everywhere or live by itself and it can be used as an alternative mosquito coil repellent.
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Repellent. It refers to a substance used to repel something (Cambridge, 2019). In
this study, this can be used in repelling such as mosquitoes/insects to prevent mosquito
bites.
This part presented the review of related literature that had relation to this study.
These literature and studies were related to this present investigation in terms of its
concept, variables, findings and other researched that supports this study. Literature
included here was related to the lemongrass and garlic as alternative natural mosquito
coil.
cuisine (Agron, 2012). Cymbopogon is a genus of about 55 species, which are indigenous
in tropical and semi-tropical areas of Asia and are cultivated in South and Central
America, Africa and other tropical countries. These are tufted perennial C4 grasses with
numerous stiff stems arising from a short, rhizomatous rootstock, as with citrus flavor,
and can be dried and powdered or used fresh. The name Cymbopogon is derived from the
Greek words “kymbe” (boat) and “pogon” (beard), referring to the flower spike
When it comes to citrusy herbs, it’s tough to beat lemongrass. This easy-growing
herb is a citrus power house. Lemongrass is a tropical herb, hardy only to Zones 8 and
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warmer. Lemongrass grows quickly and spreads to fill a planting bed or pot. Expect a
plant to reach a size of 3 to 5 feet tall and up to 2 feet wide in a growing season (Forney,
2011).
across. We cut the tops every couple of weeks because it shaded out the other herbs in the
herb bed (lots of green stuff for the compost), but it quickly grew back. And we found it
keeps the mosquitoes away. It contains something very similar to citronella oil, it's a safe
and natural insect repellent that's just as effective as the commercial chemical products,
Lemongrass Essential Oil can be helpful when carefully used in very-very low
dilution by those that are challenged with acne-prone skin. Aromatically, Lemongrass
Essential Oil blends well with essential oils in the citrus, wood, mint and herbaceous
families. Like Geranium Essential Oil and Peppermint Essential Oil, Lemongrass
Essential Oil can easily overpower a blend, so use it minimally at first. It is also a use for
muscular aches and pains, gastrointestinal disorders, indigestion, physical and mental
exhaustion, acne, insect repellent, acne, athlete's foot, excessive perspiration, flatulence,
insect repellent, muscle aches, oily skin, scabies and stress (Aromaweb, 2019).
With possible origins in India and Sri Lanka, this aromatic herb has an extensive use
throughout much of Asia as a flavorful cooking additive for salads and curries. It was
also historically used in teas, cleaning materials, perfumes, soaps, creams and deodorants.
South American folk medicine used the grass for treating hypertension, inflammation,
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nervousness, sleep disorders, infection, fevers and gastrointestinal disorders (Marshall,
2016).
Lemongrass oil is extracted from the dried leaves of the plant. It has a thin
consistency and a pale or bright yellow color. It is a great addition to various skincare,
cosmetic and industrial products such as soaps, lotions, creams and detergents. It also
works as an air freshener and deodorizer, especially when blended with other essential
oils like geranium, peppermint, cedarwood, helichrysum, ylang ylang, and conifers like
spruce, fir and pine. These blends may simply be put it in an oil burner, diffuser or
vaporizer. It is also known for its ability to repel insects such as mosquitoes and
houseflies due to its high citral and geraniol content (Mercola, 2017).
Cymbopogon citratus (Lemon grass) oil has been known as effective insect
repellent; its effectiveness in mosquito repellence has also been documented. This study
was aimed at testing the mosquito repellent property of Cymbopogon citratus on human
Cymbopogon produces the most used natural repellents in the world. Many
extracts and essential oils isolated from these plants have been tested against different
kinds of arthropods. Cymbopogon excavatus gave 100% repellence for 2 h, when it was
evaluated in the laboratory against Anopheles arabiensis and its repellence decreased to
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Garlic (Allium sativum)
Garlic is a perennial member of the onion family, Alliaceae, and is closely related
to leeks, onions, shallots, and chives. All of these plants send up hollow, tubular
(sometimes flattened) leaves from a bulb that grows below the ground. The leaves are
followed by a flower stalk (scape), and then by the flower itself. Garlic may also produce
“bulbils” – tiny bulbs that may begin to sprout, on the flower head. All parts of the garlic
plant are edible, but the bulb is the most prized and useful in the kitchen (Macdonald,
2018).
Garlic is a bulbous plant; grows up to 1.2 m in height. It is easy to grow and can
be grown in mild climates. There are different types or subspecies of garlic, most notably
thiosulfinate) is the principal bioactive compound present in the aqueous extract of garlic
activated and produce allicin from alliin (present in intact garlic) (Phytome, 2014).
In very cold regions, garlic cloves can be planted into module tray for planting out
in spring and it can be grown outdoors over winter in many regions. Garlic needs very
little attention. Water, if the weather is dry ̶ especially garlic in containers ̶ and weed
between rows to prevent plants from getting swamped. It will benefit from a mulch of
grass clippings lay an organic mulch such as grass clippings occasionally during the
growing season to help feed plants, while keeping the ground cool and moist (Vanheems,
2017).
Garlic is an herb that is grown around the world. It is used for many conditions
related to the heart and blood system. Some people use garlic to prevent and treat
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different kinds of cancers. Garlic has been tried for treating an enlarged prostate, cystic
fibrosis, diabetes, osteoarthritis, allergic rhinitis, traveler’s diarrhea, high blood pressure
late in pregnancy (pre-eclampsia), yeast infection, flu and swine flu. It is also used to
prevent tick bites, as a mosquito repellant, and for preventing the common cold, and
Eating garlic offers mild protection from mosquitoes, both from the odor on your
breath as well as sulfur compounds that you emit through your skin. The smell of garlic is
concentrations tested (Nchu, Magalo & Eloff, 2016). In the study, by the department of
Thailand affirmed that lemongrass is indeed contains organic repellents against mosquito.
Garlic bulb extract contains potential insect repellents such as thiosphens and diathiane,
and further studies to evaluate the repellent effects of the individual compounds against
There are many anecdotes and ethnobotanical claims suggesting that garlic
Garlic is used as an insecticide with both repellent and biocidal properties. Garlic
crop residues have both nematicidal and herbicidal properties. The Select Committee on
GRAS Substances (SCOGS, 1973) states there is no evidence that garlic or oil of garlic
demonstrates a hazard to the public when used at current levels (Khan & Abourashed,
2010).
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Repellents
Insect repellents are useful to prevent bites, skin eruptions and rashes that may be
caused by an insect's bite. Mosquito bites can cause severe skin irritation through an
allergic reaction to the mosquito's saliva. Authorities, such as the Center for Disease
Control and Prevention (CDC) recommend the use of insect repellents to prevent
and ethyl hexanediol. For more than 50 years, DEET has been the gold standard in
Natural anti-mosquito agents are likely to offer an inexpensive and effective that
can be used to prevent mosquitoes from human beings (Geetha, 2014). Insect repellents
work by masking humans, a number of natural and chemical mosquito repellents were
DEET is a reliable and highly effective insect repellent. The chemical has been in
public use since 1957.The repellent is sold under numerous brand names and comes in
lotion, spray and many other forms. Concentrations of DEET range from about 5% all the
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bioaccumulation (vice versa) may be an important phenomenon for toxic response of
Consumer Reports’ tests have found that some insect repellents, especially those
with the chemical DEET, can help keep bugs away. But its safety experts say that the
About 30 percent of Americans use repellents with DEET every year, but you
should know that this chemical – though generally effective in keeping away insects –
can have deadly repercussions. From 1961 to 2002, the Agency for Toxic Substances and
Disease Registry reports eight deaths related to DEET exposure (Mercola et al., 2015).
On the other hand, there is a study that lemon eucalyptus oil has been found to be
the most effective natural mosquito repellent. Studies have found that the efficacy of this
particular oil falls third in line, behind DEET and picaridin. It is the only natural repellent
that is registered with the U.S. Environmental Protection Agency (EPA) and
recommended by the Centers for Disease Control and Prevention (CDC) for effectiveness
Synthesis
From the different literature review edit was clear that the problem existed. There
were many commercial mosquito coil repellents were used and help people to repel and
eliminate mosquitoes but not good for the health. The disease brought by dengue
mosquitoes are rising up and force people to think good idea to avoid this mosquito. One
of the remedy for this problem is to make and use natural mosquito coil repellent in
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The lemongrass and garlic are known to be anti-mosquito in which they have the
smell in which mosquito repelled. Researcher (Patel, Gupta & Oswal, 2012) concluded
that lemongrass with the help of garlic can be used as mosquito repellent.
One of the studies also conclude that lemon grass can repel insect away and can
& Hailu, 2014; Reinagel et al., 2010), as stated that the smell and extract of the garlic can
repel mosquitoes.
Some insect repellents contain harmful chemicals that are not good to humans and
to the environment stated in the study of (Roy et al., 2017; Reports, 2014).
Both lemongrass and garlic were not just only use as insect repellents but they
were also used in some health medications such as in mental and physical aspects cited
The researchers affirmed the valuable results in the previous studies conducted,
hence, has found more relevance of what this present study that lemongrass and garlic
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METHODS AND MATERIALS
This part of research paper presented and discussed the different materials and
methods which were used in the scientific investigation including research design, subject
Research Design
The researcher performed the experiment using the True Experimental Design. A
True Experimental Design was regarded as the most accurate form of experimental
analysis (Shuttleworth, 2018). Also to be the only experimental design that can establish
cause and effect relationships (Yolanda, 2015). This design was used to conduct accurate
research and reliable research. This design used because the researchers only have one
experimental group and control variable, then, manipulated the variable in random
The subjects of this study were sixty mosquitoes exposed to the different set-ups
of mosquito coil repellent made from Lemongrass extract and Garlic extract. Twenty
mosquitos were under observation with two trials per set-up. Mosquitoes are small,
midge-like flies that mosquitoes prefer stagnant water within which to lay their eggs. The
most commonly infest in ponds, marshes, swamps and other wetland habitats (Tam,
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2016). The study also focused on mosquito coil repellent made from both lemongrass and
garlic extracts.
Materials
The researchers used several materials in the making of mosquito coil repellent. 2
scissors, a candle wax, an alcohol, a grinder, 2 plastic containers were used in grinding
the 1 kg. of Lemongrass leaves; Lemongrass juice was extracted with the used of metal
pot (caldero), a plastic container; Garlic was pounded with mortar and pestle and was
placed in plastic container; mosquito coil repellent was made using binder, 500g cassava
starch, a metal pot and a big plastic container. Weighing scale was also used. Small basin
and spoon was used in mixing the ingredients. For molding, a commercial coil container
was used. Microwave oven was used to dry the molded coil. Lastly, 3 set-ups of
researchers –made mosquito net was used to house the subject under study.
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Data Gathering Procedure
Collecting of Materials
Extracting of Lemongrass
Pounding of Garlic
bB
Gathering of Data
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A. Collecting the Materials
Researchers collected all the materials needed to conduct the research which
includes the following: Lemongrass, Garlic and Cassava Starch. Each mixture was
labeled carefully and placed inside the plastic containers in order to avoid mixing with
other materials. Lemongrass needed were gathered from the garden owned by Mrs. Grace
that lives six blocks from a researcher's residence, while garlics were bought from Burgos
Market where the garlic was cheaper. Plastic containers needed for storing lemongrass
extract and garlic extract were provided by Ms. Helen Mae. Cassava starch was also
Researchers also gathered all the tools and equipment needed to conduct the
research which includes the following (grinder, scissors, pestles, mortar, metal wire, and
metal pot). The following materials were used in preparing, mixing and molding the
mosquito coil. The materials mentioned above were already available in the researchers’
working place.
The researchers extracted the lemongrass to get juice that was used in the
mosquito repellent and mixed with the garlic and cassava starch to get the desired
contents needed in each Set ups. The researchers first did the cutting of lemongrass into
small pieces, and then placed in the grinder. After grinding, the researcher squeezed the
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C. Pounding of Garlic
In pounding of Garlic, the researchers used the mortar and pestle in order to
pound the garlic well and get the desired content needed and mixed with the other
materials needed in making the mosquito repellent coil. Every extracted amount of garlic
was placed in a clear plastic container with label of exact measurements. First, the
researchers peeled the garlic and pounded the garlic with moderate force using the mortar
and pestles. The pounded garlic was squeezed to get the juices and put it to a small
After the extracting and pounding of the lemongrass the researchers measured the
First, the researcher searched for lemongrass and cut it into pieces using a scissors
and used the grinder in order to get the lemongrass juice extract. And after that, the juice
of the lemongrass that has been extracted was placed in the clean plastic container.
Second, the garlics were peeled and chopped it into pieces. The researchers used the
pestle to pound the garlic in order to get the desired outcome and get the garlic juice
extract.
measurements of lemongrass juice extract and garlic juice extract. In Set-up A (3ml
lemongrass, 5ml garlic extract). In Set-up B (5ml lemongrass, 3ml garlic extract) and in
Set-up C (5ml lemongrass, 5ml garlic extract). The researchers measured the exact
amount of the lemongrass juice extract and garlic juice extract needed to produce the
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exact concentrations needed for making the alternative mosquito coil repellent. After
measuring the amounts of the lemongrass juice extract and garlic juice extract it was
placed in different clean plastic containers and mixed it with the exact measurements of
each set-ups.
After gathering and mixing the desired concentrations for each setup, commercial
mosquito coil was used to mold and formed the coil needed for experimentation. The
oven toaster is used in this process to harden the mosquito repellent coil faster. The
mosquito net cage is positioned vertically so it’s easy to know if the mosquito has been
repelled. The mosquito net cage prevented the mosquito to escape and suffocate.
The Mosquito coil repellent was tested with its specific set ups. The different
concentrations had undergone two trials with specific time given. In testing the mosquito
coil repellent, the researchers ignited the coil and put inside the net cage. Then the
researchers observed the effects of each set-up to the mosquitoes and then count the
number of mosquitos repelled in each set-up. Then, in trial two, the mosquitoes that were
F. Gathering Data
Every detail and observation was listed down in the notebook using ballpen and
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Statistical Analysis
The researchers evaluated and analyzed the data that were collected according to
All inferential statistics were tested at 0.05 level of significance and were
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RESULTS AND DISCUSSION
This section presented the key results, analysis, and interpretations of the data
gathered in the present study through conducting experiments and gathering of data about
the number of mosquitoes repelled using lemongrass and garlic against mosquitoes.
The first specific statement of the problem was to determine the mean number of
Table 1
The table showed that the Set-up C has the highest M = 19.00 and SD = 0.00 in
repelling mosquitoes. The set-up B has the lowest M = 11.50 and SD = 0.71 in repelling
mosquitoes. These implied that the mixture of 5ml garlic extract and 5ml lemongrass
extract were the most effective in repelling mosquitoes in 10 minutes of testing. The
mixture of 5ml lemongrass and 3ml garlic extracts was also effective in repelling
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There were a total of sixty mosquitoes used in the experiment. Twenty
mosquitoes were used in the first trial and the exposed mosquitoes in the first trial were
used in the second trial. This was supported by the study of Mercola (2017) that
Lemongrass has the ability to repel insects such as mosquitoes and houseflies due to its
high citral and geraniol content. Also supported by the study of Reinagel (2010) that
eating garlic offers mild protection from mosquitoes, both from the odor on your breath
as well as sulfur compounds that you emit through your skin. The smell of garlic is
Mosquito
The second specific objective of this study was to determine significant difference
Table 2 showed the significant difference among the different concentration using
One-Way ANOVA. Table 3 showed the result of the Post Hoc Tukey’s Test on the
Table 2
One-Way ANOVA Results on the Difference among the Set-ups of Lemongrass Extract
and Garlic Extract
Sum of Squares Df Mean Square F P
Between Groups 56.333 2 28.167 84.500 .002
Within Groups 1.000 3 .333
Total 57.333 5
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The result of One-Way ANOVA suggested that the difference among the different
was rejected. Therefore, there was a significant difference among the different
Since, the statistical result showed a significant difference, the researchers run the
Post Hoc Test to determine which concentrations yield the difference. Table 3 showed the
Table 3
Post Hoc Tukey’s Results on the Significant Results of the ANOVA Regarding the
Number of Mosquito Repelled of Different Set-Ups
Set-Ups(I) Set-Ups(J) Mean Difference (I-J) Std. Error Sig.
Set-Up B 4.00000* .57735 .013
Set-Up A
Set-Up C -3.50000* .57735 .018
Set-Up A -4.00000* .57735 .013
Set-Up B
Set-Up C -7.50000* .57735 .002
Set-Up A 3.50000* .57735 .018
Set-Up C
Set-Up B 7.50000* .57735 .002
Statistical result showed that there was a significant difference when one set-up
Table 3 showed that when one set-up was compared to another set-up, difference
existed. It implied that all set-ups were effective in repelling mosquitoes and just varied
as to the extent of the effect. It means that concentrations of lemongrass and garlic were
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CONCLUSIONS
The researchers present the summary of the findings of the study, draw
conclusion based from the findings of the study and give recommendations based from
the conclusions.
Set-up B and Set-up C had different levels of efficacy in repelling mosquitoes during first
On the other hand, Set-up A had the moderate level of efficacy during the first
and Set-up C.
lemongrass and garlic extracts in repelling mosquitoes were highly significant during the
The result of the Post Hoc Tukey’s Test showed that the efficacy of Set-up C in
repelling mosquitoes was significant as compared with Set-up A. The efficacy of Set-up
Based on the findings of the study, the researchers concluded the following:
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The higher the concentrations of the lemongrass and garlic extracts, the greater
the number of mosquitoes repelled. Correspondingly, the higher the concentrations, the
significantly affected the number of mosquitoes repelled during the two trials.
Based on the findings and conclusions of the study, the following were the
alternative mosquito repellent in repelling mosquito inside the house for it is safe and
effective. This will also be beneficial to home owners, store owners, and restaurant
owners and the community for it will help them repel mosquitoes in a mosquito-free
This will also help them save the environment for this is an eco-friendly and non-
toxic based.
In order for the alternative mosquito coil to be more effective, the researchers
recommended that the amount of lemongrass and garlic extracts should be the same to
Future researchers can conduct on the efficacy of the lemongrass and garlic
Lastly, studies on other potential plants with pesticide properties are encouraged.
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REFERENCES
Agron, E. (2012, June 8) Lemongrass may help repel mosquito. Retrieved from
http://www.worldngayon.com/
Formey, JM (2011) Outdoor garden grow your own garden. Retrieved from
https://www.hgtv.com/outdoors/gardens/grow-your-own-lemongrass
Goddard, J. (2002) Health Risk and Benefits of insect Repellents. Retrieved from
https://www.medscape.com
Leech, J. (2018, June 28) 11 Proven Health Benefits of Garlic. Retrieved from
https://www.healthline.com/
Marzhie Mobki et. al.,(2013,June 19) Toxicity and repellency of garlic (Allium sativum
L.) https://www.tandfonline.com/doi/abs/10.1080/03235408.2013.802896
Mercola: Take control of your health (2015).This Natural Bug Repellent Works Better
Than DEET. Retrieved from
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https://articles.mercola.com/sites/articles/archive/2015/05/25/natural-insect-
repellents.aspx
Ojewumi ME, Banjo MG, Oresegun MO, Ogunbiyi TA, Ayoola AA, Awolu OO and
Ojewumi EO: Analytical investigation of the extract of lemon grass leaves in
repelling mosquito. Int J Pharm Sci Res 2017; 8(5): 1000
09.doi:10.13040/IJPSR.0975-8232.8(5).1000-09.
https://www.researchgate.net/publication/315575384_Analytical_Investigation_of
_Extract_Of_Lemon_Grass_Leaves_Repelling_Mosquito
Patel, EK., Gupta, A., Oswal RJ. (2012) Mosquito Repellent Methods. Retrieved from
http://ijpcbs.com/
Roy et., al, (2017). The insect repellents: A silent environmental chemical toxicant to the
health. Retrieved from
https://www.ncbi.nlm.nih.gov/pubmed/28171823
Shah G. (2011) Scientific basis for the therapeutic use of Cymbopogon citratus
(Lemongrass). Retrieved from
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3217679/
Wong, A (2017) Which Natural Mosquito Repellent Works Best? Retrieved from
https://www.verywellhealth.com/natural-mosquito-repellents-88853
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APPENDICES
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APPENDIX A
Dear Sir:
We, the Grade 12 Senior high school students of Bacolod City National High School
are currently conducting our Capstone Research entitled: GARLIC (Allium Sativum)
AND LEMONGRASS (Cymbopogon Citratus) AS ALTERNATIVE NATURAL
MOSQUITO COIL REPELLENT. With your expertise, we are respectfully
requesting your good office to validate/ identify the CULICIDAE to be used in the
above study. The said subject is to be personally presented at your office with
accompanying photograph.
Your positive response on this request will be highly appreciated. Thank you and God
speed!
Respectfully yours,
Noted:
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APPENDIX B
Request Letter for Identification/Validation of the Mosquito
March 6, 2019
S I R:
The undersigned with the group are senior high school students of Bacolod City National
High School and are conducting a research study entitled: “Garlic (Allium Sativum) and
Lemongrass (Cymbopogon Citratus) As Alternative Natural Mosquito Coil
Repellent”.
With your expertise, we are respectfully requesting your good office to validate/identify
the insect/pest to be used in the above-stated study, known as the Mosquito, with
scientific name: Culicidae. Said insect is to be personally presented at your office with
accompanying photograph.
Your positive response on this request will be highly appreciated. Thank you and God
bless!
Respectfully yours,
Noted by:
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APPENDIX C
33
Appendix D
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Appendix E
Dear Ma’am/Sir:
The undersigned researcher, together with his group, are senior high school students of
Bacolod City National High School and are conducting a research study entitled: “Garlic
(Allium Sativum) and Lemongrass (Cymbopogon Citratus) As Alternative Natural
Mosquito Coil Repellent” for the fulfillment of the study.
With your expertise, we are respectfully requesting your assistance for the identification
and supervision of the different statistical tools of the aforementioned study.
Respectfully yours,
JAYCOB N. NAZARENO
Grade 12- STEM Student, Bacolod City National High School
Noted by:
35
Appendix F
CERTIFICATION
Senior High School students, had submitted their research work for verifying their
statistical tools.
Their statistical work was verified and analyzed on February 26, 2019.
Given this 26st day of February 2019 at Bacolod City National High School,
36
Appendix G
ERON A. LIRAZAN
Teacher I ̶ District Proper Custodian
Ilog Elementary School
Dear Ma’am/Sir:
The undersigned with the group, are senior high school students of Bacolod City National
High School and are conducting a research study entitled: “Garlic (Allium Sativum) and
Lemongrass (Cymbopogon Citratus) As Alternative Natural Mosquito Coil
Repellent” for the fulfillment of the study.
With your expertise, we are respectfully requesting your assistance for the text review,
proofreading, and validating grammaical content of the aforementioned study.
Respectfully yours,
Noted by:
37
Appendix H
38
Appendix I
Time Table
-making the set-ups for experiment (1st attempt) January 12, 2019
Making the coil and all the set-ups January 19, 2018
39
Appendix J
SPSS Results
ANOVA
No. of Mosquitoes Repelled
Sum of Squares df Mean Square F Sig.
Between Groups 56.333 2 28.167 84.500 .002
Within Groups 1.000 3 .333
Total 57.333 5
Multiple Comparisons
Dependent Variable: No. of Mosquitoes Repelled
Tukey HSD
(I) Set_Ups (J) Set_Ups Mean Difference Std. Error Sig.
(I-J)
40
Multiple Comparisons
Dependent Variable: No. of Mosquitoes Repelled
Tukey HSD
(I) Set_Ups (J) Set_Ups 95% Confidence Interval
41
Homogeneous Subsets
Report
Trials SetupA SetupB SetupC
Mean 16.0000 12.0000 19.0000
1.00 N 1 1 1
Std. Deviation . . .
Mean 15.0000 11.0000 19.0000
2.00 N 1 1 1
Std. Deviation . . .
Mean 15.5000 11.5000 19.0000
Total N 2 2 2
Std. Deviation .70711 .70711 .00000
42
Appendix K
Pictures
43
MOSQUITO COIL BINDER METAL PLAIN SHEET
44
JOVIELYN ZAMORA ESPINOSA
#19th Bartholomew Street, Doῆa Juliana, Barangay
Taculing Bacolod City, Negros Occidental, Philippines
Jovielynespinosa223@gmail.com
09150680356
PERSONAL DATA
Age : 18
Date of Birth : December 16, 2000
Religion : Baptist
Height : 4’6
Status : Single
Citizenship : Filipino
Father’s name : Vevincio Espinosa
Occupation : Deceased
Mother’s name : Madelyn Espinosa
Occupation : Housewife
Skills/Talents/Hobbies:
SCHOLASTIC RECORDS
45
ACHIEVEMENTS/ AWARDS/ ACTIVITIES
Senior High:
Junior High:
46
ARIEL CABARDO JAGODILLA
East Homes 2, Fortune Towne, Barangay Estefania
Bacolod City, Negros Occidental, Philippines
arieljagodilla@yahoo.com
09388101477
PERSONAL DATA
Age : 19
Date of Birth : February 11, 2000
Religion : Roman Catholic
Height : 5’4
Status : Single
Citizenship : Filipino
Father’s name : Enrique Jagodilla
Occupation : Deceased
Mother’s name : Silda Jagodilla
Occupation : Housewife
Skills/Talents/Hobbies:
SCHOLASTIC RECORDS
Senior High:
47
Representative Rubrics Cube Competition S. Y. 2017-2018
Junior High:
Primary:
48
RAINER JABAGAT MANIGOS
Purok Herbabuena, Barangay Villamonte
Bacolod City, Negros Occidental, Philippines
rmanigos@gmail.com
09103796312
PERSONAL DATA
Age : 19
Date of Birth : February 26, 2000
Religion : Roman Catholic
Height : 5’5
Status : Single
Citizenship : Filipino
Father’s name : Rodolfo M. Manigos
Occupation : Auto Diesel Mechanic
Mother’s name : Nelda J. Manigos
Occupation : Housewife
Skills/Talents/Hobbies:
SCHOLASTIC RECORDS
Luis Hervias National High School Junior High Graduated April 2017
Senior High:
49
Primary:
50
JAYCOB CARAMALES NAZARENO
Regent Pearl Subdivision, Barangay Alijis
Bacolod City, Negros Occidental, Philippines
jaycob_n@yahoo.com
09124459635
PERSONAL DATA
Age : 18
Date of Birth : August 22, 2000
Religion : Roman Catholic
Height : 5’6
Status : Single
Citizenship : Filipino
Father’s name : Lorry Nazareno
Occupation : Laborer
Mother’s name : Remedios Nazareno
Occupation : Housewife
Skills/Talents/Hobbies:
SCHOLASTIC RECORDS
Senior High:
51
Junior High:
Primary:
52
JIMRAE FORROSUELO PARAGSA
Purok Riverside, Barangay Banago, Bacolod City
Negros Occidental, Philippines
Jimraeparagsa123@gmail.com
09969910731
PERSONAL DATA
Age : 17
Date of Birth : April 18, 2001
Religion : Roman Catholic
Height : 5’5
Status : Single
Citizenship : Filipino
Father’s name : Randall T. Paragsa
Occupation : Painter
Mother’s name : Lida F. Paragsa
Occupation : Housewife
Skills/Talents/Hobbies:
I can play guitar both lean and rhythm, drums, keyboard, know some basics in
bass guitar. My hobbies are watching movies, playing instruments and I also love to play
games.
SCHOLASTIC RECORDS
La Consolacion College-
Bacolod Senior High (Grade 11) Science, Technology
Engineering and
Mathematics
April 2018
53
JAVE EARL IWAYAN QUIJANO
Purok Kametal, Barangay Bata, Bacolod City
Negros Occidental, Philippines
Earl08@gmail.com
09186460389
PERSONAL DATA
Age : 17
Date of Birth : June 8, 2001
Religion : Roman Catholic
Height : 5’6
Status : Single
Citizenship : Filipino
Father’s name : Joan C. Quijano
Occupation : Carpenter
Mother’s name : Cheza I. Quijano
Occupation : Deceased
Skills/Talents/Hobbies:
I am good at playing basketball and also, I love to play volleyball and football. I
know how to play a guitar, a little bit. I do also have skills in playing billiards.
SCHOLASTIC RECORDS
Senior High:
54
Champion Mass Dance S. Y. 2018-2019
Junior High:
Primary:
55
CHARIS NICOLE FRANCISCO
TACARDON
Leo Street, Barangay Taculing, Bacolod City
Negros Occidental, Philippines
tacardoncharisnicole@gmail.com
09476474854
PERSONAL DATA
Age : 17
Date of Birth : August 18, 2001
Religion : Baptist
Height : 4’9
Status : Single
Citizenship : Filipino
Father’s name : Jay G. Tacardon
Occupation : Laborer
Mother’s name : Lizbeth F. Tacardon
Occupation : School Clerk
Skills/Talents/Hobbies:
I am fond of eating different kinds of foods especially those that are tasty, sweet
and flavorful. I love to go out somewhere together with my family and friends while
we’re bonding, I do have lessons learned. I seldom play volleyball because it’s my
favorite sport to play not because of its benefit to make the body immune but also a quick
mind to be ready at all times. I love reading books about romantic, comedy and thrilling
stories.
SCHOLASTIC RECORDS
56
Ilog Elementary School Primary Graduated March 2013
Senior High:
Junior High:
Primary:
57
NEIL JOHN DANDO VILLAJOS
Purok Sabes, Barangay Villamonte, Bacolod City
Negros Occidental, Philippines
PERSONAL DATA
Age : 18
Date of Birth : June 22, 2000
Religion : Born Again Christian
Height : 5’7
Status : Single
Citizenship : Filipino
Father’s name : Nelson O. Villajos
Occupation : Handyman
Mother’s name : Janette D. Villajos
Occupation : NUP-PNP
Skills/Talents/Hobbies:
I love playing guitar, piano and a little bit of drums. I am fond of adventuring and
watching everything that pleases my eyes. And also, I love reading the bible.
SCHOLASTIC RECORDS
Luis Hervias National High School Junior High Graduated April 2017
Junior High:
58
Primary:
59