The document outlines topics covered in a Biophysics Syllabus:
1) Radioisotope techniques including radioactive decay, half-lives, counters, and applications in biology.
2) Centrifugation principles, types of centrifuges and rotors, and analytical and preparative ultracentrifugation methods.
3) Molecular biology techniques including nucleic acid isolation, purification, electrophoresis, sequencing, PCR, and applications in molecular diagnostics and DNA fingerprinting.
Genetic Data For 17 Y-Chromosomal STR Loci in "Macedonians" (FYRo'M' Slavs) in The FYR of "Macedonia" (FSI Genetics, Aug 2011, Volume 5, Issue 4, Pages E108-E111)
The document outlines topics covered in a Biophysics Syllabus:
1) Radioisotope techniques including radioactive decay, half-lives, counters, and applications in biology.
2) Centrifugation principles, types of centrifuges and rotors, and analytical and preparative ultracentrifugation methods.
3) Molecular biology techniques including nucleic acid isolation, purification, electrophoresis, sequencing, PCR, and applications in molecular diagnostics and DNA fingerprinting.
The document outlines topics covered in a Biophysics Syllabus:
1) Radioisotope techniques including radioactive decay, half-lives, counters, and applications in biology.
2) Centrifugation principles, types of centrifuges and rotors, and analytical and preparative ultracentrifugation methods.
3) Molecular biology techniques including nucleic acid isolation, purification, electrophoresis, sequencing, PCR, and applications in molecular diagnostics and DNA fingerprinting.
The document outlines topics covered in a Biophysics Syllabus:
1) Radioisotope techniques including radioactive decay, half-lives, counters, and applications in biology.
2) Centrifugation principles, types of centrifuges and rotors, and analytical and preparative ultracentrifugation methods.
3) Molecular biology techniques including nucleic acid isolation, purification, electrophoresis, sequencing, PCR, and applications in molecular diagnostics and DNA fingerprinting.
Radioisotope techniques: Basic concepts (types of radioactive decay, rate of radioactive
decay, radioactive isotopes and their half-lives and units of radioactivity); GM and scintillation counter; autoradiography; specific activity of a radioisotope; safety aspects; applications of radioisotopes in biological sciences. Centrifugation: Basic principles; different types of centrifuges; types of rotor; analytical and preparative ultracentrifugation methods.
Molecular biology techniques: Isolation of DNA and RNA, purification and
quantification of nucleic acids; Electrophoresis of nucleic acids: agarose gel electrophoresis, pulse field electrophoresis; capillary electrophoresis; microchip electrophoresis; DNA sequence analysis methods: Sanger dideoxy method, Maxam Gilbert chemical method and Fluorescence method; Polymerase chain reaction: principles, process, design and optimization; different types of PCR: allele specific, nested, multiplex and real-time PCR; ligase chain reaction; SNP and application in molecular diagnostics; DNA fingerprinting: applications and prospects; restriction fragment length polymorphism (RFLP) and its uses.
Spectroscopy: Nature of electromagnetic radiations; principles of biophysical methods
used for analysis of biopolymer structure - UV, Visible, Infrared, Raman, Fluorescence and NMR spectroscopy; ORD and CD; Atomic absorption spectroscopy.
immunoblotting; immunofluorescence assays; cytotoxic assay; hybridoma technology for production of monoclonal antibody - principles, techniques and applications; designing chimeric and humanized antibodies; vaccines: types and their role in prevention of diseases.
a) Concepts of precision and accuracy in experimental measurements. Concept of signal to
noise ratio. b) Biochemical Methods:
c) Chromatography: Ion exchange, Gel Filtration and Affinity chromatography.
Electrophoresis: Native and SDS-PAGE. Isoelectric focusing. 2D-PAGE and its applications. d) UV/Vis spectrophotometry: Beer-Lambert‟s law and its use in determination of protein/ nucleic acid concentration. e) Fluorescence Spectroscopy: Basic concepts of excitation and emission. Quenching, Stern-Volmer Plots. Theory and applications of FRET and fluorescence lifetime measurements. f) Fundamentals of CD, IR and Raman spectroscopy and their use in the study of biomolecular conformation. g) Centrifugation: Basic concepts of centrifugation. Calculation of g value from RPM. Density gradient centrifugation. Sedimentation velocity and Sedimentation equilibrium. Separation of sub-cellular components and macromolecules using high speed and ultracentrifugation. h) Microscopy: Bright field, phase contrast, fluorescence, confocal, and electron microscopy. i) Fundamentals of X-ray, NMR and cryo-electron microscopy for determination of biomolecular structure.
Genetic Data For 17 Y-Chromosomal STR Loci in "Macedonians" (FYRo'M' Slavs) in The FYR of "Macedonia" (FSI Genetics, Aug 2011, Volume 5, Issue 4, Pages E108-E111)