Marija Atanasova

[11C]CO2 + H2  [11C]CH4
I2
• Loading of Target with
N2+O2
• Irradiation with [11C]CH3I
Cyclotron protons Transfer of
Target content to
Methylator (module
for syntehsis)

Methylator
CarbonSynthon

Dispensing
• Dose for patients
Clio

[11C] Choline
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Converting the Transfer the
Trapping C02 Purifying the The Production produced Synthesis [11C]
[11C]CO2
[11C]Me of [11C]MeI [11C]MeI Choline
to [11C]Me

First four steps are synthesis of [11C]MeI

[11C]MeI

Fifth step is
transfer the
produced
[11C]MeI
CrabonSynthon
Methylator
Synthesis of 11C-Choline
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Trapping of 11CO2

[11C]CO2 is being trapped on

to Molesieve 4A from the
cyclotron

When all activity is trapped

button should
be pressed
Synthesis of 11C-Metyl Iodine
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Converting the [11C]CO2 to [11C]Me
 Reduction of the [11C]CO2 with H2
into [11C]Me. This is catalyzed by
Shimalite Ni
 During the reduction process the
excess of H2 is being flushed away.
All remaining H2 is being held inside
the reactor of the Methanizer.

The reactor must be put at least 350 0

C to perform the reduction at least 2.5
minutes.
The [11C]CO2 is being released from the
Molesieve 4A at about 180 0C.
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Purifying the [11C]Me

Once released from either
Methanizer the [11C]Me is
being transported through
Carbosphere valve V03 to the purifying
column containing
Carbosphere or Carboxen
1000.

The H2 will flow 7 times as

fast through the
Carbosphere as does the
[11C]Me.
This gives rise to a nice
opportunity to separate
the two gasses.
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The Production of [11C]MeI

The moment to switch back valve V04 , тhe

[11C]Me is directed to the Iodine oven and
for this reason valve V05 is switched as well.

 The [11C]MeI is coming through the Iodine oven and will mix with the sublimated Iodine (I2). In the following High Temperature oven,
the two components will react into [11C]MeI and HI. The flow will transport the reactants through the exhaust tube were excess of
Iodine will be deposited.
 The produced [11C]MeI will be transported with the He flow toward the Porapak oven. The Porapak oven contains Porapak N which is
able to trap the [11C]MeI.
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Transfer the produced MeI to CarbonSynthon

The flow from flow

controller 1 will keep the I2
concentration constant
Once all activity resides on the Porapak
oven, the Iodine oven will be separated from
the Porapak
oven, the flow path that is. The Porapak
oven will be heated, while a message box
will give you a message that the [11C]MeI is
ready. The He will start to flow once both
the message box is clicked away and the
temperature is high enough (180-190 0C).

The flow controller 2

will have separate
access to the Porapak
oven
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Choline Synthesis
Reaction between MeI with DMAE Synthetized MeI through V19 is reacting with precursor DMAE
(dimethylamino ethanol).

[11C]CH3I

Produced 11C Choline

Precursor DAME is loaded is retained on the
on SePak column (cation column as a positive
exchange column) molecule
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Choline Synthesis
Purifying the produced choline

Washing the unreacted

DMAE and other impurities
are wash out with water into
waste

Produced 11C Choline is

retained on the column
as a positive molecule
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[11C]Choline Synthesis
Elution of 11C- Choline

Elution of 11C Choline with 0.9%

NaCl transfer in dispensing unit
In process controls in synthesis of MeI

CarboAct
MethAct

PoraAct
The shielded cell series, mod. BBST, is expressly dedicated to filling, calibrating and fractioning operations on vials/syringes

Work chamber
Classified air - Class А
• Clio inside
• Dose calibrator for measuring the
dose
Pre-chamber • Holder for vials and syringes
Class B

Connection layout between

chambers

Shielded container
for syringe and vial
Drawing system transport
Dispensing unit Clio has been designed and manufactured for the semi-automatic dispensation of
radiotracers
The system uses a single production sterile kit, which allows
working with high flexibility, dispensing materials into the
following types of container
• Closed vials
• Open vials
• Syringes

Radiopharmaceutical
Bulk
Saline solution
Technical gas He
Dispensing needle