9/21/2019 Preclass Quiz 8: Fa19 - MOLECULAR BIOLOGY (47940)

Preclass Quiz 8
Due Sep 24 at 11am Points 10 Questions 10 Available Sep 20 at 5pm - Sep 24 at 11am 4 days Time Limit None

Attempt History
Attempt Time Score
LATEST Attempt 1 25 minutes 10 out of 10

Score for this quiz: 10 out of 10

Submitted Sep 21 at 4:24pm
This attempt took 25 minutes.

Question 1 1 / 1 pts

Which of the following is mostly likely to occur when sporulatiom occurs in B. subtilis?

activation of vegetative genes

reduction of endospore formation

Correct! complex sigma-switching

complete shutdown of transcription

deletion of vegetative genes

Question 2 1 / 1 pts

Which of the following conditions does not lead to a change in gene expression patterns in bacterial cells?

nutrient availability

sporulation

Correct! none of the choices are correct.

heat shock

nitrogen deprivation

Question 3 1 / 1 pts

Hairpin formation is necessary for ____________________.

base paring between DNA and RNA

elongation

initiation

Correct! termination

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9/21/2019 Preclass Quiz 8: Fa19 - MOLECULAR BIOLOGY (47940)

Question 4 1 / 1 pts

Which of the following techniques can be use to study the interaction between repressors and operators?

x-ray diffraction

immunoblotting

Electrohoretic Mobility Shift Assay

Correct!
Both x-ray diffraction and Electrohoretic Mobility Shift Assay

Southern blotting

Question 5 1 / 1 pts

Protein crystals are used in x-ray diffraction studies because

Correct!
the molecules of proteins in a powder or solution are too loose, the diffraction power of a single protein molecule is too low, and several molecules of
proteins are in a crystal leading to strong diffraction patterns.

the molecules of proteins in a powder or solution are too loose.

the diffraction power of a single protein molecule is too low.

several molecules of proteins are in a crystal leading to strong diffraction patterns.

the molecules of proteins in a powder or solution are too loose and the diffraction power of a single protein molecule is too low only.

Question 6 1 / 1 pts

You have just discovered a new putative repressor in a strain of E. coli that was sent to you by a colleague. One of the best ways to test
for the specificity of this repressor for an operator sequence is to

replace the putative DNA-binding site with the binding site from another repressor.

replace the putative DNA-binding site with the binding site from another repressor and change some of the amino acids in the binding site by site-
directed mutagenesis.

Correct!
change some of the amino acids in the binding site by site-directed mutagenesis.

insert an additional segment of amino acids next to the DNA binding site.

change some of the amino acids in the binding site by site-directed mutagenesis and insert an additional segment of amino acids next to the DNA
binding site.

Question 7 1 / 1 pts

Compared to binding of monomeric proteins to DNA, which of the following is true regarding the binding of a dimeric protein?

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9/21/2019 Preclass Quiz 8: Fa19 - MOLECULAR BIOLOGY (47940)

disorder increases

Correct!
requires less energy

none of the choices are correct.

requires twice the energy

entropy increases

Question 8 1 / 1 pts

DNA binding proteins are usually octamers.

True

Correct!
False

Question 9 1 / 1 pts

Heat shock genes encode proteins that lead to the rapid death of cells when they are exposed to heat.

True

Correct!
False

Question 10 1 / 1 pts

Transcriptional changes that occur when organisms respond to environmental changes are in part due to changes in RNA polymerase.

Correct!
True

False

Quiz Score: 10 out of 10

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