COLLEGE OF AGRICULTURE,

GWALIOR

PROJECT PLANNING TO ESTABLISH A

TITLE-
COMMERCIAL PROPAGATION UNIT FOR
PRODUCING PAPAYA PLANTS.

SUBMITTED TO- SUBMITTED BY-

DEPARTMENT OF PMB & BHUPENDAR PATIDAR
BIOTECHNOLOGY BSC (AG) IVTH Yr
ROLL NO. 151A06
 EVALUATION : 1
Project Planning to Establish Commercial
Propagation Unit

OBJECTIVE :-
Planning to produce 50,000 Papaya plants through Tissue Culture.

TARGETED PLANT :- Papaya

INTRODUCTION
Scientific name- Carica papaya
Family- Caricaceae

 Papaya are the fruits or berries of plants belonging

to the caricaceae family .
 Capsicum annuum is the most economically
 important and includes both mild and pungent
fruit types
 Papaya is also known as “melon tree”
-Papain milk is obtained from papaya plants.
-Papain has several industrial uses such as in
brewing industry, in meat industry it is used as a
meat tenderizer , in drug making and in textile
industry used as a sanforization.
 Enzyme present in dried latex of papaya is
pepsin.
NAME OF SOME IMPORTANT PAPAYA VARITIES:-
 GYNODIOECIOUS VARITIES
1. Pusa majesty(nematode resistance)
2. Coorg honey dew
3. Co-3
4. Surya
5. Sunshine solo
6. Pusa delicious
 DIOECIOUS VARITIES
1. Pusa gaint
2. Pusa dwarf
3. Co-5
4. Hatras gold

REQUIREMENTS FOR PROJECT PLANNING FOR

PRODUCING 50,000 PLANTS OF PAPAYA BY TISSUE
CULTURE
EXPLANT- Meristem tip or Apical bud
GENERAL REQUIREMENT-
Glassware-

 Culture tube
 Measuring cylinder (10,50, 100,500ml &1L)
 Beakers (50,100,500&1000ml)
 Conical flask (100,500&1000ml)
 Petri dishes
 Bottles (for media storage & for propagation)
 pipettes (1,2,5,10ml)
PLASTICWARES-
*Plastic carboys
*Baskets
*Water bottles
*Aluminium foil

EQUIPMENTS-
 Analytical balance
 Distill water unit
 Laminar air flow
 Autoclave
 Hot air oven
 Hot plate
 Mechanical shaker
 Magnetic stirrers
 Microscope
 Temperature controller
 Electronic timers
 Humidifiers
 Sprit lamp

EQUIPMENTS-
 Analytical balance
 Distill water unit
 Laminar air flow
  Autoclave
 Hot air oven
 Hot plate
 Mechanical shaker
 Magnetic stirrers
 Microscope
 Temperature controller
 Electronic timers
 Humidifiers
 Sprit lamp
 Air conditioners
 Filter paper
 pH meter
 Refrigerator
 LPG cylinder

Chemicals- Generally MS Media is used for micro

propagation of papaya.
MACRONUTRIENTS(mg/L)
MgSo4.7H2O - 370

KH₂Po4 - 170

KNO3 - 1900

NH4NO3 - 1650

CaCl₂.2H2O - 440
MICRONUTRIENTS(mg/L)
H3BO3 - 6.2
MnSo4.4H2O - 22.3
ZnSo4.7H2O - 8.6
Na2MoO4.2H2o - 0.25

CuSo4.5H2O - 0.025

CoCl₂.6H2O - 0.025

KI - 0.83

FeSO₄. &H2O - 27.8

Na2EDTA. 2H2O - 37.3

Sucrose(g) - 20
ORGANIC SUPPLEMENTS VITAMINS
Thiamine HCL - 0.5

Pyridoxine (HCL) – 0.5

Nicotinic acid - 0.5

Myo inositol - 100

METHODOLOGY

Explant (Shoot tips)

↓
Surface sterilization
(0.1% HgCl2 solution containing tween 20 for 3-4 min)
↓
Washing
(3-4 times with sterilized distilled water.)
↓
Shoot initiation
(MS media + 0.5mg/L BAP + 1mg/L Kn + 0.3 % (W/V)
activated charcoal) for 3-4 weeks
↓
Shoot proliferation
(MS-media + 2mg/L BAP + 0.1mg/L NAA + 0.5mg/L GA3)
+
Shoot elongation
(MS-media + 0.5mg/L GA3) for 3 weeks
↓
Rooting
 (3-4 cm elongated shoots in MS-media + 2mg/L IBA + 2mg/L
IAA) for 3 weeks
↓
Incubated in growth room
(Temp.- 24-28 degree celcius , 16/8 hours light/dark , 3000
lux light intensity)
↓
Hardening
(4-5 roots of length 3-5 cm washes with warm water and
transplanted to plastic pots of diameter 10cm containing
sterile peat moss.)

BUDGETING –
Operating cost:

1. Management & staff Rs1,25,000

2. Utilities Rs1,00,000
3. Insurance/Maintenance Rs75,000
4. Chemical & other consumables Rs50,000
5. Miscellaneous cost Rs33,500
6. Depreciation (10%) Rs42,000
Total Rs4,25,500
 Economics –
1. Total selling price Rs 6,50,000
2. Total expenditure Rs4,25,500
3. Net profit Rs2,25,000

Cost of papaya tissue culture plant is Rs 13 per plant so price

of 50,000 plants is Rs6.50 Lakh. Operating cost is Rs4,25,500.
Benefit cost Ratio = Total Return
Total Expenditure
BCR = 2,25,000
4,25,500
BCR = 0.52 or 52%

CONCLUSION
Commercial propagation of papaya would be profitable as it
is in demand and is mostly consumed in every house of the
country. It is known for its health benefits. Papaya fruit is
always on demand on market because it is not costly and
great in taste. Thus the commercial propagation of papaya
would be of great use as well as profitable.