Bioassay for Diphtheria

antitoxin; Tetanus; Cholera vaccine; Posterior Pituitary extract; Adrenaline;

Heparin; Digitalis; d-Tubocurarine; Vitamins. Test for pyrogens.

Bioassay methods for autocoids – Development of new bio-assay methods.

Assays using special designs for experiments to eliminate known source of
variation. Toxicity tests, Determination of LD50, Acute, Sub acute, and
Chronic toxicity studies – Tests for freedom from undue toxicity of drugs.

Bioassays : Basic principles of bioassays, official bioassays,

experimental models and statistical designs employed in biological
standardization. Biological standardization of vaccines and sera,
vasopressin, oxytocin, Acetylcholine, Adrenaline, insulin,
d-tubocurarine, HCG, hyaluronidase, corticotrophine, pertussia, rables
and plague.

List of bioassays

1. Bioassays of hormones
a. Insulin
b. HCG
c. Corticotrophin
d. Vasopressin
e. Oxytocin
f. Androgen
g. Estrogen
h. Progesterone
i. Growth hormone
j. Gonadotropins
i. FSH
ii. LH
2. Bioassay of vitamins
a. Vit B12
 b. Vit D
c. Vit B6
3. Bioassays of vaccines & sera
a. Diphtheria
b. Gas gangrene
c. Rabies
d. Tetanus
e. Pertussis
f. Thyroid vaccine
g. Cholera
h. Plague
4. Bioassays of autacoids
a. Histamine like substances
b. 5 hydroxytryptamine
5. Bioassay of some important drugs
a. D-tubocurarine
b. Digitalis
c. Adrenalin
d. Nor-adrenalin
e. Acetylcholine
f. Heparin
g. Hyaluronidase
6. Bioassay of antibiotics