BEER’S LAW

CALIBRATION CURVE
Problem 1: The determination of iron in an industrial
waste stream is carried out by the o-phenanthroline
method. Using the data in the following table,
determine the mg Fe/L in the waste stream. Construct a
calibration curve and formulate your equation.
The data for a standard solution of 0.0250 M Cr3+, a standard solution of
0.0750 M Co2+, and a mixture that contains unknown concentrations of each
ion are shown here.

Use a multiwavelength regression analysis to determine the composition of the unknown.

SEATWORK:
ASSIGNMENT PROBLEM 1:
To evaluate the determination of copper and zinc in tissue samples, a series of external
standard is prepared and analyzed, providing the results shown:

A bovine liver standard reference material is used to evaluate the method’s accuracy.
After drying and extracting the sample, a 11.23-mg FFDT tissue sample gives an
absorbance of 0.023. Report the amount of copper in the sample as µg Cu/g FFDT. 5ml of
sample is used.
ASSIGNMENT PROBLEM 2:

Plot the data (designate the x-axis for wavelength). The optimum wavelengths are those
where the ratio of the absorptivities has its maximum and its minimum value.
ASSIGNMENT PROBLEM 3:

Determine the concentration of titanium and vanadium.