KARAN WARUDKAR

MD TAUSIF
KARTHIC P
UJWAL LIMJE
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 ADVANTAGES
● Simple and fast technique

● It’s suitable for large- scale separations

LIMITATIONS

● CD34 KG-I cells contaminated with Jurkat cells

● Using the whole technique again can improve the purity of CD34 cells
 - A technique that deals with the behaviour, precise control and
Microfluidics manipulation of fluids that are geometrically constrained to a
small, typically sub-millimeter, scale at which capillary penetration
governs mass transport

- A common application is DNA chip technology: Detection of

gene/gene expression in an array of DNA strands

- Advantages:
- Low volume fluids
- High throughput screening: large no of compounds can be
tested for activity
- Faster analysis and response times - due to short
intermolecular distances
- Automated assessment of quality

- Disadvantages:
- Detection may not scale down positively (noise is much
more enhanced at this scale)
- Surface dependent effects like capillary forces, surface
roughness or chemical interactions are more dominant
Microfluidics in stem-cell separation
● SELEX microfluidics:
○ Number of nucleotide sequences that can be possibly formed increases exponentially with
sequence length.
○ Selection of aptamers require a trial and error of a large number of sequences with structural
diversity.

● Affinity-based microfluidic cell separation:

○ Uses fluid flow for separation
○ Microfluidic devices coated with antibodies have been demonstrated to selectively capture
mouse adipose-derived stem cells from a heterogeneous suspension
○ The release of molecules is enabled by application of shear stress
○ Affinity is highly enhanced in this technique due to increased surface-volume ratio
Thank You