UPH-DR. JOSE G.

TAMA YO MEDICAL UNIVERSITY

COLLEGE OF MEDICAL TECHNOLOGY

IMMUNOLOGY-SEROLOGY

ACTIVITY 10
A . RHE~MATOID ARTHRITIS TEST (RA)
Rapid Latex Shde Test for Detection of Rheumatoid Factor \'\u"
f:,
LEARNING OBJECTIVES:
th
_ 'f
At e co n clusion of this activity, the student should be able to: '
1 · know th e diagnostic procedures used in the evaluation of rheumatoid arthritis
2
- know the immunologic manifestations of rheumatoid arthritis.

PRE-LAB DISCUSSION
(RheumatoidArt~ (RA) is a chronic, multisystemic, autoimmune disorder and a
progressive mflamm_atory_disorder of the:~ Early symptoms 6f ~ t 2in and ~ular
s~ ss may occur at any age wit~ ci:la~ qµvI.P.ri»P.ntly affected than)IIJlles The disease
~ ately pro.gr~ ~ ~ver~ k~let al deforrmbfa~ _<i_~ Pl ete immo~ilizat~ L th~ff~

li!:1£ul_mato~-fac!~:-sfR£) are ~ ~ ar.a.p,.ainly_a,g~t the Fe region of

lgG and react w1tb gamwa...q l2,bulins of patients with rheumatoid arthritis. -
Principle of this Latex Slide Test is based on an immunol ogic reaction between the RF
found in the test serum and the corresponding human lgG bound to laQlvsfyceoe late~ parti-
cp When te,:,.i...;:,ei•l:04-I.I. containing rheumatoid factors is mixed with the RF Latex reagent, visi-
ble a~ ccurs

REAGENTS:
RF Latex Reagent: A suspension of latex particles sensitized with specially prepared human
lgG in glycine Saline buffer.
RF Positive Control Serum
RF Negative Control Serum
Glycine Concentrate (20X) - concentrated Glycine Saline Buffer is to be diluted 1 20 with
purified water. (For quantitative test)

MATERIALS
Serological pipette - 1 ml Applicator stick
Aspirator bulb Test tubes 13 x 100 mm
Transparent glass slide or concave ring slide Pasteur pipette
Stirring pipettes(provided in the kit)

SPECIMEN: Unhemolyzed and fresh clear serum

PROCEDURE:

NOTE: Allow reagents and sera to room temperature.

Shake the RF-Latex Reagent gently to ensure a uniform suspension before using

Place one drop of the test specimen onto a glass slide using a separate
plastic stirring pipette for each specimen . _
2 On a separate glass slide place one drop each of negative and positive
control using separate plastic stirring pipette for each control.
3 Shake well and gently dispense one drop of the RF-Latex Reagent directly
onto each specimen and controls.
4 Stir with the flat end of the stirring pipette until the mixture of the specimen
and the controls with the latex reagent is spread over the entire field of the
nng
5 Tilt the shde back and forth , slowly and evenly 8 to 10 times per minute for
3 minutes Place the slide on flat surface and observe immediately for
macroscopic agglutination using a direct light source .

26
 UPH-DR. JOSE G. TAMAYO MEDICAL UNIVERSITY
COLLEGE OF MEDICAL TECHNOLOGY
IMMUNOLOGY-SEROLOGY

INTERPRETATION OF RESULTS:
NEGATIVE TEST- a uniform milky suspension with no agglutination within three
(3) minutes as observed with the negative control .
POSITIVE TEST- any observable agglutination in the reaction mixture with in
three(3) minutes as observed with the positive control Non
specific reactions may occur after 3 minutes.

An agg_lutination of the latex particle suspension is a positive result The strength of

the pos1t1ve reaction may be graded as follows :

1+ = Very small clumping with an opaque fluid background

2+ = Small clumping with slightly opaque fluid background
3+ = Moderate clumping with a fairly clear fluid background
4+ = Large clumping with a clear fluid background

Note: Sera with positive results in the screening test should be retested 1n the
quantitative test. Please refer to the kit's leaflet for the quantitative procedure.

EXPECTED VALUES:
Distinct agglutination indicates a RF content of more than 20 IU/mL in the undiluted
sample.

RF may frequently be present in Scleroderma, Still's disease. Sjorgren's syndrome

and systemic lupus erythematosus.

ILLUSTRATE YOUR RESULTS

Patient serum

Positive Control

Negative Control

L
 UPH-OR. JOSE G. TAMAYO MEDICAL UNIVERSITY
COLLEGE OF MEDICAL TECHNOLOGY
IMMUNOLOGY-SEROLOGY
ACTIVITY 11
SYSTEMIC LUPUS ERYTHEMATOSUS (SLE)
LE-SLIDE TEST
LEARNING OBJECTIVES:
At the conclusion of th1 s Activity. the student should be able to:
I know the clinical applications of the SLE screening test.
2. know the significance of the demonstrable serum antibodies that r9act to SLE
3 enumerate ~he different laboratory diagnosis manifested through histologic.
hematologic And serolog1c abnormalities of SLE.

PRE-LAB DISCUSSION
Systemic Lupus Erythematosus 1s the classic model of autoimmune disease . The
cause of SLE is unknown(idiopathic). A primary defect in the regulation of the immune system
1s considered important in the pathogenesis of the disorder
SLE is a disease of acute and chronic inflammation. Symptoms of SU: often mimic
other less serious illnesses. Manifestations of the disease range from a typical mild illness lim-
ited to a photosensitive facial rash and transient diffuse arthritis to life-threatening involvement
of the 1-enal. cardiac, respiratory , or central nervous systems.
Patients with SLE are known to produce multiple autoantibodies. Laboratory fea-
tures of SLE are the presence of ANA. immune complexes. complement level depression. tis-
sue deposition of lgGs and complement, circulating anticoagulants and other autoantibodies

REAGENTS:
LE Latex Reagent-a suspension of uniform polystyrene particles sensitized
with DNP(rabbiVfetal calf thymus) in glycine buffer
LE Positive Control
LE Negative Control

MATERIALS
Serological pipette - 1 n,L Applicator stick
Aspirator bulb . Test tubes 13 x 100 mm
Transparent glass slide or concave ring slide Pasteur pipette

Unhemolyzed and fresh clear serum

SPECIMEN:

PROCEDURE:
NOTE: Allow reagents and sera to room temperature. f
Shake tile LE-Latex Reagent gently to ensure a uni orm suspension before using

Place one drop of tile test specimen onto a glass shde using a separate
losltc stirring pipette for each specimen
2
bn
a separate glass slide place one drop each of negative and pos1t1vo
control using separate plastic stirring pipette for each control
Shake well ond gently dispense one drop of the RF-Latex Reagent directly
3
nto each specimen and controls
~t with the not end of the stimng pipette until the mixture of the specimen
~~ the controls w1tll the latex reagent ls spread over the ontire field of thQ
8
4

;'.~~the slide back And forth, slowly and evenly 8 to 10 times per minute for
0
3
mlnutn Place the sl_1de on_Ost su~nce ?nd observe Immediately for
macroscopic agglutination U!;1ng a direct hght source
Drying or tile rn1xture rn oy lead to erroneous result, the result:-. therotoro
Id be examined for no longer than 3 m1nutos
ShOU

29
 UPH-DR. JOSE G. TAMA YO MEDICAL UNIVERSITY
COLLEGE OF MEDICAL TECHNOLOGY
IMMUNOLOGY-SEROLOGY
INTERPRETATION OF RES UL TS:
NEGATIVE TEST- a uniform milky suspension with no ~gglutinat,on within three
(3) minutes as observed with the negative control
POSITIVE TEST- any observable agglutination ,n the reaction mixture w,th,n
three(3) minutes as observed with the pos1t1ve control Non-
specific reactions may occur after 3 minutes.

Note: Sera with positive results in the screening test should be retested in the
quantitative test Please refer to the kit's leaflet for the quantitative proce dure

Biologic false positive results can be manifested by disorders such as

systemic lupus erythematosus, Sjogren's syndrome, syph11ts and hepat1t1s.

EXPECTED VALUES
Distinct agglutination indicates Anh-DNA is usually present Ant1-DNP may be
found in diseases other than SLE . Low titers have been detected ,n RA. SJogren's
syndrome . periarteritis nodose. dermatomyositis. scleroderma and lymphoma

ILLUSTRATE YOUR RES UL TS

Patient Serum

•
I •

Positive Control

Negative Control
 UPH-OR. JOSE G. TAMAYO MEDICAL UNIVERSITY
COLLEGE OF MEDICAL TECHNOLOGY
IMMUNOLOGY -SEROLOGY

PROCEDURE :

NOTE: Allow test casette and serum to room temperature.

Wear protective gloves while handling the spcimens.
Avoid splashing or aerosol formation.

1. Remov e the test cassette from the fo;J pooch and place on a flat.dry surface
2 U$ing a automatic micropipeite. add 100 ul of specimen ITT to tr.e sample
well mar1<ed T.
3. As the test begins to ...,'Ofk. you wi!l see purple colDf' move across the resu!t

------=~ window in the center of the tre4wst cassette.

4 . Sample Well Interpret results after 20 minutes
100 uL

Sam~e Well
100 uL
HBsAg C T

DO NOT READ RESULTS AFTER 30 MINUTES

INTERPRETATION OF THE RESULTS

NON-REACTIVE REACTIVE

C T C T
HBsAg HBsAg

0) ~ _I1_1_
The preffllce of only one purple ~ band ~ presence ot two purl)le colo( baflds
(·C"band) n the resul window 111.dteates f'T band -C"bandl in ~ rtt~ window
a non-reactiv~ res.utt indkatts a ~ • rtsult

INVALID RESULT

C T

If no C ~ 11 dew~ rtw ttsay ,s \n"a!td

~ s ot M'IY pu,ple ~ band '11 the T band.

Repelt the test.

The resu.11 I! c o , ~ !JWMd
I MW c ~ ~e"Vlee.
33
 UPH-DR. JOSE G. TAMAYO MEDICAL UNIVERSITY
COLLEGE OF MEDICAL TECHNOLOGY
IMMUNOLOGY-SEROLOGY

ACTIVITY 13
s HUMAN IMMUNODEFICIENCY VIRUS (HIV)
D-HIV-1 112 IMMUNOCHROMATOGRAPHIC SCREENING TEST
LEARNING OBJECTIVES:
At the conclusion of this activity. the student should b bl t ·
1
· know th e latest diagnostic procedures usedei: th: :~aluation of HIV.
2 know the immunologic manifestations of HIV.
3 know the confirmatory test for HIV determination.
PRE-LAB DISCUSSION

. Human imm_ u nodeficiency virus (HIV-1) is the predominant virus responsible for ac-
quired ni:1munodefic1ency syndrome (AIDS). HIV is a member of the family Retroviridae , a type
D retrovirus that belongs to the lentivirus subfamily .. Included in this fam ily are oncoviruses
(e.g), HTLV-1 and II that primarily induce proliferation of infected cells and formation of
tumors .
HIV-1 virus is composed of a lipid membrane, structural proteins and glycoproteins that
protrude . The viral genome consists of three important structural components : pol. gag. and
env ..
HIV-1 has a marked preference for C04+ subset of lymphocytes because the CD4 sur-
face marker protein on these cells serves as a receptor site for the virus.

PRINCIPLE
The SD-Bioline HIV- 1/2 test contains a membrane strip, which is precoated with
recombinant HIV-1 capture antigen (gp41, p24) on test band 1 region and with recombinant
Hl\/-2 capture antigen (gp36 on test band 2 region , respectively. The recombinant HIV-1/2
antigen (gp42, p24 and gp36) colloid gold conjugate and the specimen sample move along the
membrane chromatographically to the test region (T) and form a visible line as the antigen-
antibody gold particle complex forms with high degree of sensitivity and specificity. This test
device has a label of 1,2 and C as Test line 1 (HIV-1 ) , Test line 2 (HIV-2) and control line on
the surface of the device. The presence of three lines as control line C , test hne 1 ( 1) and test
line 2 (2) within the result window indicates a positive result for HIV-1 and/or HIV-2 .

REAGENTS:
SD-BIOLINE HIV -1/2 Test Device
Assay diluent
MATERIALS
Serological pipette - 1 ml Pasteur pipette
Aspirator bulb Test tubes 13 x 100 mm

SPECIMEN: Unhemolyzed and fresh clear serum

Wear protective gloves while handling specimens .
PROCEDURE:

1 Remove the test device from the foil pouch, place it on a flat, dry surface
2 Add 1o µI of plasma or serum specimen in the sample well (s)
3 _Add 4 drops (about 120 µI) of as~ay diluent in\o th~ sample well (s) h
4 _As the test begi ns to work. you WIii ~ee a purp e co or move across t .e result
window in the center of the test device
5 Interpret the results in 5 to 20 minutes.

NOTE Do not read test results after 20 minutes Reading too late can give false pos ,trve
rec;ults

INTERPRETATION OF THE TEST

, A color band will appear ,n the left section of the result window to show that the test
it working property This band 1s control line C
2 Color bands will appear ,n the middle and nght section These bands are lest hnc 7
i:lnd t est line , (2 1)
35
 UPH-DR. JOSE G. TAMA YO MEDICAL UNIVERSITY
COLLEGE OF MEDICAL TECHNOLOGY
IMMUNOLOGY-SEROLOGY

Negative Result
. The presence of only the control line (C) within the result window indicates a
negative result .

Positive Result
1 · The presence of two lines as Control line (C) and test line 1 ( 1) within the
result window indicates a positive result for HIV-1
2 . The presence of two lines as Control line (C) and test line 2 (2) with in the
result widow indicates a positive result for HIV-2
3. The presence of three lines as Control line (C) test line 1 (1) and test line 2
(2) within the result window indicates a positive result for HIV-1 and/or HIV-2

NOTE: A positive result should be confirmed with other laboratory diagnostic tests such as
Western Blot Test or PCR.

Invalid Result
No presence of control line (C) within the result window indicates an invalid result . The
directions may not have been followed correctly or the test may have been deteriorated. It 1s
recommended that the specimen be re-tested .

C
II
NEGATIVE HIV-1 POSITIVE HIV-2 POSITIVE

HIV-1/HIV-2 POSITIVE

ILLUSTRATE YOUR RESULTS

Patient Serum (HIV-1/2)

NEGATIVE POSITIVE

36

J
 UPH-DR. JOSE
COLLEGEGO. TAMAYO MEDICAL UNIVERSITY
F MEDICAL TECHNOLOGY
IMMUNOLOGY-SEROLOGY
ACTIVITY 14
DENGUE DENGUE
Two-stop Assa f DUO NS1 + lgG/lgM Combo Casette Test
Y or detection of lgG or lgM antibodies to Dengue Virus
LEARNING OBJECTIVES:
At the conclusion of thl~ activity, the student should be able to:
1· ~now !~e ~mmunochromatogrnphic principle of the combo rapid test for dengue
now ie 1_mportance of detecting NS1 antigen and lgG and lgM antibodies of
dengue virus . ·

PRE-LAB DISCUSSION
The Dengue virus belongs to the Flavivirus group of viruses. This is a virus commonly
found throughout the tropics and Africa.
The Flaviviridae are a family of viruses that are primarily spread through arthropod vec-
tors (mainly ticks and mosquitoes(Aedes)). The family gets its name from Yellow Fever virus, a
type virus of Flaviviridae; flavus means yellow in Latin. The diagnosis of dengue is usually

The Dengue NS1 antigen and lgG/lgM test kit is a rapid membrane based screening
test to differentially detect the presence of NS1 antigen and antibodies to Dengue virus . NS1 is
a highly conserved glycoprotein thatis present at high concetratrions in the sera of dengue-
infected p[atients during the early clinical phase of the infection. NS1 antigen is found from the
first day and up to 9 days after onset of fever of primary and secondary dengue-infected pa-
tients.
Usually lgM does not become detectable until 5 to 10 days after the onset of infection
in cases of primary dengue infection and until 4 to 5 days after onset of 1Uness in secondary
infections . In primary infection, lgG appears on the 14 day and persists for life.

PRINCIPLE . ·
This Dengue Duo Rapid test is an i.rrimunochromatograph1c_one_step assay des1qnd
to detect both dengue virus NS 1 antigen and differential lgG/lqM ant1bod1es _to dengue w us
Thisa test 1s intended to be used as an aid in the presump-
m serum , pIasma or w ho Ie blood . . .
. . b tw primary and secondary dengue infection.
live d1af~~s;:st ~s t~:nnewer generation lateral flow immunochromatographic type assay
. t nd easiest to use POC (point of care) assays.
These are among the simp1es _ah 'th serum or whole blood. The test employs the use of
This test can b~ used eit ~r ;; colloidal gold particles and a unique combination of
antibody binding protein~ _conJuga ~
0
mbrane to form a complex of antibodies-antigens.
Dengue antigens immobilized on t e me

REAGENTS/KIT COMPO~E~~: lgG/lgM combo casette devices

Dengue NS 1 g I G/lgM test
Assay dilu_e nt for o~;eg~~ 6engue lgG/lgM test
1O ul capillary pipe for Dengue NS 1 Ag test
Disposable dropper
d fresh clear serum , plasma or whole blood
Unhernolyzed an

Lr•
SPECIMEN:
____.,. Sample well
Result window

~
----► Assay diluent well

.
,~ .
>"I'
. J- .
!
lgMllgG
C1sette "
__ _ _.,. Result window

NS1 Antigen
Cas.ette t L• _ _ __ _ _,..., Sample woll

38
 . . - · '• ..,v->c '-i. r AMA YO MEDICAL UNIVERSITY
COLLEGE OF MEDICAL TECHNOLOGY

IMMUNOLOGY-SEROLOGY

PROCEDURE
I. DENGUE CASSETTE
1 U sing the capillary p' tt ·
mart<.ed "S" _ ipe e provided, add 10 ul of seruminto the sample well
2 Di$pen1e 4 drops of ass d' I ·
t ay I uent into the round shape assay diluent well
3 1n erpret re6ults after 20 minutes
4
Do not re ad results after 20 minutes as this will give a false result

\
( .
l'

INTERPRETATION OF RES ULTS

lgM POSITIVE lgG POSITIVE

The presence of two lines in "C" and
"M" in the result window Indicates a
positive result for primary infection.
It is positive even if "M" line is weak.
lgM/lgG POSITIVE
The presence of two lines in "C" and
"G" in the result window indicates a
positive result for secondary
or past infection.
It is positive even if "G" line is weak.

C'~-~1 1
' Id:
1 , o~ .~ ~-~
) ~-- - ---- - - - - - - ---'
I
The presence of three lines In "C" The presence of only one line In
" M" and "G" in the result window "C" in the result window indicates
indicates a positive result for a negative result for
Late primary or early secondary
dengue infection

INVALID RESULT: . h "M" d "G,., in the

The resence of ONE or TWO lines ln either or bot · an
result !.ndow but no line in the "C" window Indicates an invalid result.
Retest the specimen again.
39
 UPH-DR. JOSE G. TAMAYO MEDICAL UNIVERSITY
COLLEGE OF MEDICAL TECHNOLOGY
IMMUNOLOGY-SEROLOGY

PROCEDURE

I\. NS'\ ANTIGEN CASETTE

1. Using the disposable dropper pro'llded. dispense 3 drops lapprox. 100 ul) into
\he sample well of the NS1 cassette
2 . Interpret results after 20 minutes.
3. Do not read results after 20 minutes as this will gi"e a false result.

INTERPRETATION OF RESULTS

NS1 POSITIVE

,.,_ __/ - l
CT ~ \

\.
I
____ ' '
C 2 1 ~ -J

\or bands in the "T"

The presence of two co It
and "C" line indicates a POSITIVE resu

INVALIO RESULl
NEGATIVE

r ~

l r t>and in
The absence of a co o
one color the " C'' line \nd\cates
Th e Presence of on1y . n \NV ALID result .
. "C'' rne
I indicates a ecimen again.
band m the It Retest the sp
a NEGATIVE resu

40

l
 l"AMAYO MEDICAL UNIVERSITY
· · ""' " · "'vvt: \,:;,
COLLEGE OF MEDICAL TECHNOLOGY
IMMUNOLOGY-SEROLOGY
ACTIVITY 15
MALARIA
Pf/Pan ANTIGEN TEST
Rapid Test for Malaria
LEARNING OBJECTIVES:
At the conclusion of this activity, the student should be able to:
1. k~ow the principle serological assays for malaria.
2 d1fferent1ate the different Plasmodium species.

PRE-LAB DISCUSSION
Four .species of the Plasmodium parasites are responsible for malaria infections in hu-
man: P. falc,parum , P. vivax, P. ova/e and P. malariae. Of these P. falciparum and P vivax are
the mo_ s t prevalent. Early detection and differentiation of malaria is of paramount importance
due to incidence of cerebral malaria and drug resistance associated with falciparum malaria
Malaria Pf/Pan Antigen test is a rapid self-performing, qualitative, two site sandwich
immunoassay, utilizing whole blood for the detection of P. falciparum specific histidine nch pro-
tein-2 (Pf HRP-2) and pan specific pLOH. This test may also be used for differentiation of P
falciparum and other malarial species and for the follow-up of anti-malarial therapy in whole
blood samples.
Malaria Pf/Pan Antigen test detects the presence of pan malaria specific pLOH released
from the parasitized blood cells, for the detection of all malarial parasites. Whereas, for the de-
tection of P. falciparum malaria. Malaria Pf/Pan Antigen test utilizes the detection of P. falcipa-
rum specific histidine rich protein-2 (Pf HRP-2) which is a water soluble protein that is released
from the parasitized erythrocytes of infected individuals.

PRINCIPLE .
Malaria Pf/Pan rapid test utilizes the principle of i~mu~ochromatograp~y. The detection
test device (whole blood) contains a membrane stnp, which 1s a precoated with two monoclo-
nal antibodies as two separate ;lines across a test strip: One mo~oclonal antibody test (TEST
LINE 1) · specific to the P. falciparum histidine rich protein-2 (Pf HRP-2) and another
is . T LINE 2) is pan to the lactate dehydrogenase (LOH) of
monoclonal ant1b~dy test (T~S . malariae and ovale). Conjugate pad is dispensed
Plasmod1um species (P:. falciparum_,dv;vaxld which are specific to P. falciparum histidine rich
with monoclonal ant1boa1es to colloi a go ' . ·
protein-2 (Pf-HRP-2) and pan to the LOH of Plasmod1um species.

REAGENTS/KIT COMPO.NENTS . ·
A. Individual pouch~s, each conta1rnns~embly predispensed with monoclo~al ant1-HRP- :L
1 Test device: Membran~~noclonal anti pan specific pLDH-collo1dal gold con1u•
colloidal gold conJugate, . con·u ate and monoclonal anti Pf HRP-2 anti-
g ate
,
rabbit globuhn-collo1dal gol_
dfi
lonal anti pan spec, ,c P
Lb~antibody and anti-rabbit anhbody at the
body, mon~c
specific regions.
L capillary pipette ..
2. 5 µ . a dropper bottle
B Clearing buffer in

used as a test sample and EDT A or

SPECIMEN: . gulated whole blood should be t oagulant Handle all specnnens
Fresh ant1-coa be used as suitable an ic
xalate can · I es
Heparin or O · wear protective g ov ·
·a11y infectious.
35 potent 1

42
 1
\ _ ~' IMMUNOLOGY-SEROLOGY
PROCEDURE:
1 Bnng the Malana Pf/Pan Antigen test kit compononts to room tompo,all;ro
betore testing
2 Open the pouch and retncve the test cosette. Once opened, tho casottc muot bo
used immediately
3. Add 5 ul of whole blood into tho sample well marked "$1" (the small woll)
4 Add 3 drops (approx ) 80 ul) of assay buffer in the developer rn nrkod "$" (the b,g
well)
5 Interpret the results after 20 minutes.
Finger Prick Method (Collect ion using a lancot)
1. Clean the area to be punctured with an alcohol swab.
2. Squeeze the end of the fingertip and pierce with a sterile lancet
3. Wipe away the first drop of blood with sterile gauze or cotton
4 Using a 5 ul capillary pipet provided, gently squeeze the pipette and ,mmcrse the
open end in the blood drop. Then gently release the pressure to draw blood from
the pipette.
5. Start with the procedure above..

5 µ(

0I ... .....
_.. .,
~

--
-----.;
I,.......... •

-- d
I
• .. ~ ,(_J_d_ro_ns--,.J READ RESULTS
,~
·- AFTER 20

8 i~
I

~
,. f MINUTES
~'
f
ct3 ~
--
---
,. . . •..,1!
...
I
INTERPRETATION OF RESULTS
NEGATIVE

M11l11rla
POSITIVE
,
Mtfarta
( M11l1rlll
I
-
I.'
l
,l
I.. C
,,... ron
( ... : .. . pl
I • ••• f}..U1

: -· o·
 ..,,
. -r~, 1 1-Ut<. JOSE G. TAMAYO MEDICAL UNIVERSITY
COLLEGE OF MEDICAL TECHNOLOGY
'1- ~i
~', IMMUNOLOGY-SEROLOGY
'~~
pROCEDURE:
1 Bnng the Malana Pf /Pan Antigen test kit components to room temperature
be1ore testing.
2. Open the pouch and retneve \he test casette Once opened, the casette must be
used immediately.
3 Add 5 ul of whole blood into the sample well marked "S1 " (the small well)
4 . Add 3 drops (approx.) 80 ul) of assay buffer m the developer marked "S" (the btg
well)
5 Interpret the results after 20 minutes.
Finger Prick Method (Collection using a lancet)
1. Clean the area to be punctured with an alcohol swab.
2 . Squeeze the end of the fingertip and pierce with a sterile lancet.
3. Wipe away the first drop of blood with sterile gauze or cotton.
4 . Using a 5 ul capillary pipe\ provided, gently squeeze the pipette and immerse the
open end in the blood drop .. Then gently release the pressure to draw blood from
the pipette .
5 Start with the procedure above ..

l
---- J .;:-
READ RESULTS
AFTER 20
MINUTES

f _ I
G ':-wi
INTERPRET1'T\ON OF RESULTS
NEGATIVE
POSITIVE

\, ~•l~w
I1: .,.rl, Malarla

I ~ \
<.
I .. •.-- ::liltl
<
,.~.~n
·--c;»!"
! ·- · c!
. . ...... ,t • .•. . ol

nr prnn<'~ uf n,o n,e prc-s.-on 1.11 O'l \

0 ' 0 . tolol· l)AIMI -( •
c>)k)r ba ..h -c- ,-_,.i
l 'IK~~o-f 1n_(Jk.-lC'i _. ~ •a ll"'
• 1- 11l'1~1r II p<n-111\ t
ii HO I , -.Wl'!I t-., 11td• r~II IOI I' bl, 11-YI 'llil
l ...i,,"...- J ~ 1'>C f ~
wr l'A1' -LOI\
,--.e-•I\
aoJ ll'l!i.• , ~...,.
(.}!M'f" l~:.~aot.11•• l
•i.,l1 1 ~ I l.il,"lr:,-naGl
:O-\l«IIO or
~ r .._ , .1.nH
a,111 ,r• k \f41 ru • I
1:1') . . ~ tn 1c-cll0ftll )