Check Lists

Spectrophotometer Laminar Air Flow

 Pen  Pen
 Record book  Record book
 Pendrive  Permanent markers, fine & bold
 Tray  Tray
 Tissue roll  Pipettes 2, 20, 200, 1000
 Discard bin  MCT Rack
 Cuvette - 2  Distilled water bottle
 0.85 % Autoclaved Saline  Iso-propanol (Spray bottle)
 Pipette – T 1000  Tissue roll
 Tip box – 1000 µL  Gas Catridge
 MCT Rack  Meta-Loop
 Samples  Sodium hypochlorite
 Spreaders
 Media
 Antibiotics, Sugars, Supplements
 Petri-plates
 Discard bin

Sonication Gel Imaging

 Pen  Pen
 Record book  Record book
 Tray  Tray
 Samples  Tissue Roll
 Distilled water  Pen-drive
 Isopropanol  Discard bin
 Tissue Roll  Agarose gel/SDS PAGE gel
 Ice in Beaker  Distilled water
 Discard
 Pipette – T 200, T 1000
 Tip box – 200 µL, 1000 µL
 Discard
 Lysis buffer
Reagent Preparation Check List
1. Media
 LB agar
 LB broth flasks
 LB broth test tubes (15 & 50 mL)
 TS agar
 TS broth flasks
 TS broth test tubes (15 & 50 mL)
 Auto-induction broth
2. Probiotic media
 Lactobacillus agar
 MRS agar
3. MiliQ water (50 mL falcon), 1 L flask
4. TE buffer (50 mL falcon)
5. Physiological saline (0.85% NaCl)
6. Microcentrifuge (Eppendorf) tubes
 1.5 ml
 0.5 ml
7. Tip boxes
 1ml
 0.2ml
 0.01 ml
8. Washed falcons
 50 ml
 15ml
9. L-Spreaders
10. Oakridge 50 mL tubes
11. Centrifuge bottles
 500 mL
 250 mL
12. Empty glassware
 White cap (50 mL) tubes
 Yellow cap (15 mL) tubes
 250 mL bottle
 500 mL bottle
 250 ml (empty cotton-plugged) flasks
 500 ml (empty cotton-plugged) flasks
13. Any buffers (New or Running low)
14. Any media, Antibiotics, Sugars, Supplements (New or Running low)