Muntinlupa Science High School Procedure

Research 2
Third Quarter 1. Cover the open ends of washed and dried
Activity / Quiz # 1 glassware with cotton plugs and wrap in butter
Sterilization and paper/ Aluminum foil. Don’t forget to write your
Basic Apparatus / Equipment in Microbiology name on your materials.

Name: __________________________ Section: 2. Wrap stainless steel sampling scoops

(inoculating loop/ needle) in aluminum foil.

3. Cover the petri-plates with the lid and place

them in the stainless-steel petri-plate
containers or wrap four petri-plates in butter
paper or aluminum foil.

4. Wrap glass ware/apparatus, which are to be

I. Sterilization
Sterilization (or sterilization), referring to any
process that eliminates (removes) or kills
(deactivates) all forms of life and other biological
agents (as well as viruses which some do not
consider to be alive but
are biological pathogens nonetheless), including
transmissible agents (such as fungi, bacteria, viruses,
spore forms, unicellular eukaryotic organisms such
as Plasmodium, etc.) present in a specified region,
such as a surface, a volume of fluid, medication, or in
a compound such as biological culture media.
Sterilization can be achieved with one or more of the
following: heat, chemicals, irradiation, high
pressure, and filtration. Sterilization is distinct
from disinfection, sanitization, and pasteurization in
that sterilization kills, deactivates, or eliminates all
forms of life and other biological agents. Sterilization
can be achieved by physical, chemical and
physiochemical means. Chemicals used as sterilizing
agents are called chemosterilants.
autoclaved, such as filtration units, forceps and
scissors with butter paper/Aluminum foil.
5. Place all material to be sterilized in the
autoclave/ steamer.
6. Close the door of autoclave / steamer and
operate the autoclave/steamer as per the
instructions given in Cleaning and Operation of
Auto Sliding Door Sterilizer. Maintain the
temperature of autoclave (121 0C for 20
minutes.) /steamer at 100 0 C at 45 mins.
7. Open the door of autoclave when the
temperature of chamber is less than 90oC.
8. Using cotton hand gloves, remove the sterilized
glass ware/ apparatus from the
Autoclave/steamer.
9. Write on the sterilized glassware the date of
sterilization, load number.

II. Applications
10. Store sterilized glassware/apparatus in a clean
1. Food
dry place.
2. Medicine & Surgery
3. Spacecraft
Challenge Based on the video clip
Sterilization of Glassware and presented to you during your
Apparatus Used in Microbiology laboratory lecture answer the
Laboratory following questions:

Materials 1. What is the purpose of sterilization?

Petri dish 2. Differentiate the two types of sterilization.
Test-tube 3. Enumerate and describe the different method
Inoculating loop and Needle of sterilization.
Aluminum Foil / Oslo Paper 4. What are the two types of radiation describe
Cotton and Gauze each?
Empty clean Lab Kit per group 5. What is the difference between sterilization and
Steamer (for steaming method) disinfection?
Test tube Holder
Marker and tape
 III. Basic Apparatus and Equipment in 4. Autoclave
Microbiology Picture Function

Microbiology and parasitology utilize many different

laboratory apparatus and equipment. Microbiological
and parasitic studies and experiments employ such 5. Laminator Flow
techniques as sterilization, isolation of bacteria, culture Picture Function
media preparation, and biochemical testing among
others. And these cannot be performed without these
tools.

A. Complete the table below. Paste picture and give

the function or use of each apparatus/ equipment.
1. Microorganism Incubator
Picture Function

6. Top Loading
Picture Function

2. Hot Plate
Picture Function

7. Glass Slide with Cover

Picture Function

3. Water Steam Bath

Picture Function

8. Wash Bottle
Picture Function
9. Inoculating Needle and Loop 13. Erlenmeyer Flask
Picture Function Picture Function

10. Stirring Rod 14. Test tube & Test Tube Rack
Picture Function Picture Function

Adapted to Microbiology & Parasitology LabManual by Howell Ho

11. Alcohol Lamp et.al
Picture Function

12. Petri Dish

Picture Function