F.

3 Biology
Topic: Cell Structure – Use of Microscope
Suggested Answers
Skills for microscopic examination
(a) Low-power magnification
a. Nosepiece e. Clips
b. Eyepiece f. Coarse adjustment
c. Condenser g. Body tube
d. Stage h. Fine adjustment

(b) High power magnification

i. Low-power
j. Centre
k. Nosepiece
l. Illumination/ light condition

Practical: Examining onion epidermal cells

Results:

Discussion:
1. To avoid the trapping of air bubbles and prevent the overlapping of epidermal
cells.
2. The iodine solution acts as a stain to make some structures more conspicuous
(to show up more clearly).
3. It reduces water loss of the specimen and prevents the objective lens from
getting wet.
4. The cheek cells have no cell wall, but the onion epidermal cells have cell
walls; the cheek cells are irregular in shape, but the onion epidermal cells are
 regular in shape.
Light Microscope Exercise Suggested Answer
1. a W: eyepiece 1m
X: objective 1m
Y: stage 1m
Z: condenser 1m
b i 10 x 10 = 100X 1m
ii 8/16 = 0.5 1m

2. a For selecting different objectives to give different magnifications. 2m

b It is held in position by the pair of clips located on the stage 2m
c It reflects light to the specimen. 2m
d By adjusting the diaphragm. 2m

e To prevent breaking the objective lens and / or the slide. 2m