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B. Habitat and Niche: Concept of habitat and niche; niche width and overlap; fundamental
and realized niche; resource partitioning; character displacement.
Genomics and its application to health and agriculture, including gene therapy.
Biosensors
UNIT: 13 Methods in Biology
A. Molecular Biology and Recombinant DNA methods
Isolation and purification of RNA, DNA (genomic and plasmid) and proteins,
different separation methods. Analysis of RNA, DNA and proteins by one and two
dimensional gel electrophoresis, Isoelectric focusing gels.
Molecular cloning of DNA or RNA fragments in bacterial and eukaryotic systems.
Expression of recombinant proteins using bacterial, animal and plant vectors.
Isolation of specific nucleic acid sequences Generation of genomic and cDNA
libraries in plasmid, phage, cosmid, BAC and YAC vectors. In vitro mutagenesis
and deletion techniques, gene knock out in bacterial and eukaryotic organisms.
Protein sequencing methods, detection of post translation modification of
proteins. DNA sequencing methods, strategies for genome sequencing.
Methods for analysis of gene expression at RNA and protein level, large scale
expression, such as microarray based techniques Isolation, separation and
analysis of carbohydrate and lipid molecules RFLP, RAPD and AFLP techniques.
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