Test for the Presence of Biomolecules

Embudo, Helen Gail; Milano, Ian Harold; Pecson, Joyce Ann; Santua Welaiza

BS Biology 1A

Biology Department College of Science

Bicol University Legazpi City

Aims: To investigate the presence of biomolecules in the samples provided and differentiate the results
of the tests for biomolecules.
Study Design: Scientific method
Place and Duration of the Study: Laboratory room 108, Biology Department, College of Science,
August 19, 2019.
Methodology: This experimentation is composed of four procedures: Iodine Test where a tincture of
Iodine solution was dropped in the potato; the Sudan III Test for Lipids where a sample of fatty pork
meat was soaked in 70% ethanol and Sudan III; Heating 1.5 ml of egg white solution and 1.5 ml of
sugar solution in 30 seconds; and Ethanol Emulsion for Fats and Oils that was done by crushing fatty
meat sample and soaking it with 70% ethanol solution then afterwards decanting it on another tube with
2 ml. of distilled water.
Results: The results were acquired from 4 different experiments conducted in the laboratory. The iodine
test revealed starch composition in potato, specifically the reaction of amylase to the iodine solution; the
Sudan III Test as a lipid indicator for the fatty meat sample; the formation of cloudy-white texture in
albumen (egg white) after heat application as an indication for protein composition in the egg solution
and the emergence of bubbles to indicate the boiling point of water in sugar solution.
Conclusion: The experiments yielded the presence of biomolecules in different substances by
performing certain or specific test that would allow these biomolecules to react. These finding also
verified the presence of biomolecules in our surroundings especially in the food normally eaten by
humans.

Keywords: biomolecules, Sudan III test, Iodine test, Ethanol emulsion, lipids, starch, protein
1. INTRODUCTION

Modern scientific thought is founded largely on a tendency to subdivide each process or

structure into ultimate units, also known as reductionism. By studying or understanding small parts,
scientists expect to comprehend the whole.
The cell, which is the smallest and fundamental form of life responsible for all life processes,
is composed of molecules and substances both organic and inorganic.