Volibris & Flolan Mechanism of Action Animation Storyboard

Brief: The purpose of this animation is to show how Volibris and Flolan act to treat
PAH. Elements from the PAH disease state animation could be used for the
introduction. The rest of the animation should focus on the action of these agents on
endothelial and smooth muscle cells within the walls of the pulmonary arteries.

Onscreen Text Description Example image

Introduction
The raised Start with
pulmonary arterial image of the
blood pressure lungs and
and resistance heart, zoom to
seen in patients right
with PAH is a pulmonary
result of artery
imbalances in
molecules that
cause…
…vasoconstriction, Image
or tightening, of showing the
the artery… blood vessel
wall
contracting
and lumen
narrowing
[does not
have to be 3D]
… and increased Image
growth, or showing
proliferation, of the growth of the
smooth muscle inside of the
cells in the blood blood vessel,
vessel wall making the
causing further channel
narrowing, narrower
[does not
have to be 3D]
The blood flow Show
from the heart to pulmonary
the lungs is artery
restricted narrowing

Volibris section
Endothelin-1 is a Show
molecule produced molecule
by the endothelial labelled
cells of blood Endothelin-1
vessels appearing
outside
endothelial
cells
Endothelin-1 binds Show two
different
to two receptors in coloured but
blood vessels same shaped
receptors on
endothelial and
smooth muscle
cells. Be sure
that the
endothelium
and smooth
muscle cells
are well
differentiated
(as shown,
there are no
ETA receptors
on the
endothelium but
both ETA and
ETB receptors
are found on
the smooth
muscle cells).

Endothelin-1 Endothelin-1
binding to ETA and molecule
ETB receptors on binding to ETA
smooth muscle and ETB
cells causes receptors on
vasoconstriction the smooth
and cell growth muscle cells

Endothelin-1 Endothelin-1
binding to ETB molecule
receptors on binding to ETB
endothelial cells receptors on
results in the
vasodilation, or endothelial
relaxation, of the cells
smooth muscle
cells
Volibris treats PAH Molecules
by selectively labelled
binding to ETA Volibris binds
receptors and to ETA
blocking the action receptors,
of endothelin-1 show
endothelin-1
trying to bind
to but
bouncing off
Volibris-bound
receptor
As Volibris has Blood vessel
blocked the ETA expands,
receptor, expansion of
vasoconstriction the inside wall
and cell stops/reduces,
proliferation is increased
stopped. However, blood flow
beneficial
vasodilation
occurs due to
binding of
endothelin-1 to the
ETB receptor