ATTN:

Humaclot Junior Procedure

PT

1. Pipette 25ul plasma into cuvette.

2. Prewarm plasma for 1 min
3. Transfer cuvette to measuring position.
4. Activate optic (press key “optic”)
5. Add 50ul prewarmed thromboplastin (measurement starts automatically).
6. The instrument will read for maximum of 300s. If no clot is detected, the display will read “+++.+ s.”
7. The result is displayed in seconds and INR.

PTT

1. Pipette 25ul plasma into cuvette.

2. Add 25ul APTT to plasma.
3. Incubate exactly for 5 minutes (if APTT-P is used), or incubate exactly for 3 minutes (if APTT-EA is
used).
4. Transfer cuvette to measuring position.
5. Activate optic (press key “optic”)
6. Add 25ul prewarmed Calcium Chloride (the measurements start automatically)
7. The instrument will read for a maximum of 300s. If no clot is detected, the display will read “+++.+ s”
8. The result is displayed in seconds ratio

FIB

1. Pipette 50ul plasma dilution (1:10 with IBS or Owrens buffer) into cuvette
2. Prewarm plasma for 1 min.
3. Transfer cuvette to measuring position
4. Activate optic (press key “optic”)
5. Add 25ul fibrinogen reagent (the measurement starts automatically).
6. The instrument will read for a maximum of 60s.
7. The result is delayed in seconds and mg/dl.

TT
1. Pipette 50ul plasma sample into cuvette
2. Prewarm plasma for 1 min
3. Transfer cuvette to measuring position
4. Activate optic (press key “optic”)
5. Add 50ul bovine thrombin (the measurement starts automatically).
6. The instrument will read for a maximum of 300s.
7. The result is displayed in seconds and ratio.