B.E.

(Biotechnology) Fourth Year-Seventh Semester (Syllabus)

BIO701 Environmental Biotechnology

Total Number of lectures: 45 Maximum marks: 100

Note for the Paper setter: The question paper should be divided into Section A and
Section B Total of 8 questions, 4 from section A and 4 from section B are to be set.. The
students will be required to attempt 5 questions selecting at least 2 from each section.

SECTION-A
Introduction: Environmental Pollution, Sources and effects. Biodegradation and
Bioremediation- definitions and examples. (5)
Waste Water Treatment: Aerobic and Anaerobic waste water treatment. Kinetics for waste
water treatment processes. Different type and design of Aerobic and Anaerobic reactors,
Methanogenesis. Emerging biotechnological processes in waste water treatment. (18)

SECTION-B
Solid Waste management: Biodegradation of pollutants, Treatment processes for solid waste,
Use and Management, Bioremediation of contaminant lands. Hazardous wastes: Source
management and safety. (10)
Microbial Leaching and Mining: Recovery of metals from solutions, microbes in petroleum
extraction, microbial desulfurization of coal. (7)
Environmental Genetics: Degradative plasmids, release of genetically engineered microbes in
environment. (5)

Practicals:
To determine BOD & COD levels of the sample. Application of microbial approaches in the
remediation of contaminated soils. Analysis of contaminated and bioremediated samples.

Reference Books
1. Bruce E. Rittmann & Perry L. McCarty. Environmental Biotechnology: Principles &
Applications (1st Edition), McGraw-Hill, 2000.
 2. Michael T. Madigan, John Martinko & Jack Parker. Brock Biology of Microorganisms
(10th Edition). Prentice Hall, 2002.
3. George Tchobanoglous, Franklin L. Burton Editor & H. David Stensel Wastewater
Engineering: Treatment and Reuse (4th Edition). McGraw-Hill, 2002.
4. Comprehensive Biotechnology (Vol. 1-4): M. Y. Young (Eds.), Pergamon Press, Oxford.

Paper Code : BIO 702 Food Biotechnology Max.Marks:100

Course duration: 45 Lectures of one hour each.

Note : The question paper should be divided into Section A and Section B Total of 8 questions, 4
from section A and 4 from section B are to be set.. The students will be required to attempt 5
questions selecting at least 2 from each section
SECTION-A

History of Microorganisms in foods (2)

Primary sources of Microorganisms in foods (3)
Synopsis of common food borne bacteria, fungi and yeasts (6)
Incidence & Behaviour of Microorganisms in foods (3)
Extrinsic & Intrinsic parameters of foods (4)
Role and Significance of Microorganisms as Single cell proteins, Food value of Mushrooms,
Yeasts, Production of Fermented foods (3)

SECTION-B
Food types and their physical & chemical properties (4)
Food Spoilage (3)
Food Borne diseases (6)
Food Preservation (5)
Diagnosis of microbial contents of food: Classical & Molecular approach (4)
Food Biosensors (2)

Practicals:

Microbiological Examination of food/s. Enumeration and detection of food borne organisms.

Estimation of quality of milk-by dye reduction, direct microscopic count, Determination of
diacetyl, titrable acidity in the milk sample.

Reference books

1. Morten Meilgaard, Gail Vance Civille & B. Thomas Carr. Sensory Evaluation
Techniques (3rd Edition). CRC Press, 1999.
2. Daniel Charles. Lords of the Harvest: Biotech, Big Money, and the Future of Food (1 st
Edition). Perseus Books Group, 2001.
3. Adams, M.R. and M.O. Moss. Food Microbiology. Turpin Distribution Service Ltd.,
Blackhorse Road, Letchworth, Herts SG6 1HN, UK, 1995.
4. Gauri Mittal.Food Biotechnology: Techniques and Applications. CRC Press, 1992.
5. Banwart, George J. Basic Food Microbiology, 2nd ed. AVI/ Van Nostrand Reinhold
Publishing Co, 1989.
6. Cliver, D.D. Foodborne Diseases. Academic Press, Inc, 1990.
7. Food and Drug Administration. Bacteriological Analytical Manual (BAM) (8th Edition).
AOAC, Arlington, VA, 1995.

BIO703 Plant Tissue Culture

Total Number of lectures: 45 Maximum marks: 100

Note for the Paper setter: The question paper should be divided into Section A and
Section B Total of 8 questions, 4 from section A and 4 from section B are to be set.. The
students will be required to attempt 5 questions selecting at least 2 from each section.

Section A

Introduction, requirements and Techniques. (4)

Tissue Culture Media and Cell Culturing. (5)
Cellular Totipotency, Somatic Embryogenesis, Synthetic seeds. (6)
Haploid Production: Zygotic Embryo Culture: Morphogenesis in the culture of seeds with
partially differentiated embryos. (5)

Section B

Microsurgical experiments, Morphogenic potential of the embryo callus, practical

applications. (5)
In-Vitro Pollination and Fertilization: Introduction, Terminology, In-Vitro Pollination,
Applications. (5)
Genetic engineering and production of pathogen free plants: Gene expression, genetic
stability, and field performance (5)
Introduction, Strategies used to optimize product yield, commercial aspects, Germplasm
storage: Introduction, long term Storage, Short or Medium term storage. (10)

Reference Books:

1. Adrian Slater, Nigel W. Scott & Mark R. Fowler. Plant Biotechnology: The Genetic
Manipulation of Plants (1st Edition). Oxford University Press, 2003.
2. Roberta H. Smith. Plant Tissue Culture: Techniques and Experiments (2 Edition).
Academic Press, 2000.
 3. Lydiane Kyte, John Kleyn & John G. Kleyn. Plants from Test Tubes: An Introduction to
Micropropagation (3rd Edition). Timber Press, 1996.
4. J. Heslop-Harrison, John H. Dodds & Lorin W. Roberts. Experiments in Plant Tissue
Culture (3rd Edition), Cambridge University Press, 1995.

BIO 704 Bioanalytical Techniques

Max. marks: 100 Time:3 Hours

Course Duration : 45 lectures of one hour each.

Note for the paper setter: The question paper should be divided into section A and section B.
Total of 8 questions, 4 from section A and 4 from section B are to be set. The students will be
required to attempt 5 questions selecting at least 2 from each section.
Section-A
Spectrophotometry(UV and Visible): Principle, single beam and double beam
spectrophotometer, factors influencing the absorption spectra, overview of empirical rules,
solvent perturbation method and difference spectroscopy; various applications of absorption
spectroscopy w.r.t. biotechnology.(05)
Spectrofluorimetry: Principle, significance and various details related to instrumentation. (03)
Atomic absorption Spectrophotometry: Principle, instrumentation details, various
interferences in atomic absorption spectroscopy and applications.(04)
Infrared and Raman Spectroscopy: Principle, factors deciding the spectra, instrumentation,
overview of different class of compounds and their IR spectra. Introduction to Raman scattering.
(04)
Nuclear Magnetic resonance: phenomena of resonance, instrumentation, diamagnetic
shielding, anisotropy, chemical shift, free induction decay (FID), population distribution of
nuclei, and prediction of NMR spectra on the basis of (n+ 1) rule for basic class of compounds.
Overview of electron spin resonance spectroscopy (ESR) and magnetic resonance imaging
(MRI).(08)
Section-B
Electron Microscopy: Transmission and scanning electron microscopy, significance of vacuum,
basic instrumentation for TEM and SEM, sample preparation for electron microscopy. Overview
of Atomic force microscopy and tunneling microscopy w.r.t. their working principle and
comparison with other scanning techniques. (05)
Crystallography and X-ray diffraction: Introduction to x-ray and general theory and
instrumentation, Bragg’s law, various techniques to determine crystal structure.(05)
Radioisotope techniques: radiotracers, units of radioactivity measurement, proportional and
scintillation counters, introduction to autoradiography and nuclear medicine.(06)
Mass Spectroscopic techniques: Introduction to mass-spectroscopy, significance,
instrumentation details of a mass-spectrometer, ionization techniques, single and double
focusing, alternate mass separation techniques- time of flight and quadruple. Interface of mass-
spectra with liquid and gas chromatography (LC-MS and GC-MS)(05)
Practicals:
Different experiments to confirm spectroscopic laws, practical demonstration of the above
mentioned techniques.
Suggested Books:
1. Keith Wilson & John Walker
2. William Kemp
3. David Friefelder
4. P. Carmona, R. Navarro,A.
: Principles and Techniques of Practical
Biochemistry (5th Edition), Cambridge University
Press,2000.
: Organic spectroscopy. English Language
Book Society & The Macmillan Press Ltd.
: Physical Biochemistry, Freeman & Co.
New York
: Spectroscopy of Biological Molecules:
Modern trends( Ist Editon), Kluwer Academic
Publishers, 1997.