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Bio 103 - 127.2 Exercise 11 Factors Affecting Bacterial Growth
Bio 103 - 127.2 Exercise 11 Factors Affecting Bacterial Growth
Bio 103 - 127.2 Exercise 11 Factors Affecting Bacterial Growth
Laboratory Exercise 11
PHYSICAL AND CHEMICAL FACTORS AFFECTING GROWTH
Objectives: At the end of the experiment, the student must be able to:
1. Determine the effects of several chemical and physical factors on the growth of different
microorganisms;
2. Classify different bacterial species according to their response to various temperature,
pH, oxygen and salinity/osmolarity.
Procedure:
A. Effect of Temperature
1. Inoculate 3 NA slants with E. coli or S. aureus.
2. Incubate the first tube at the refrigerator (4°C), the second at room temperature (28°C),
the third at the incubator and the last one at the oven (55°C).
3. Examine the slants for growth after 24 hours of incubation. Record your results as
follows: 0 = no growth, 1+ = slight growth, 2+ = moderate, 3+ = abundant. Determine
temperature classification of each bacterial species.
B. Effect of pH
1. Inoculate S. aureus into agar slants of different pH (pH 5, 7, 9).
2. Incubate the tubes at 35°C for 24 hours.
3. Examine the slants for growth after incubation. Record your results, as in A-3. Determine
the optimum pH requirement for growth of each organism.
Study Questions:
Short but concise answers must be placed after every question.
Cite your references.