Practice Test Biology Test Chapter 13 Refer

Refer to the Text Chapter 13 and Take the following Test.

True/False
Indicate whether the sentence or statement is true or false.

____ 1. Genetic engineering can be used to move genes from the chromosomes of one organism into those of another.
____ 2. Gene cloning is an efficient way to produce many copies of a specific gene.
____ 3. Restriction enzymes make a straight cut through both strands of DNA.
____ 4. The “sticky ends” of a DNA fragment can combine with any other DNA fragment cut by the same restriction
enzyme.
____ 5. Gene cloning is an efficient means of producing large numbers of different genes.
____ 6. A ring of DNA in a bacterium is called a plasmid.
____ 7. Recombinant DNA is usually composed of DNA segments obtained from two different organisms.
____ 8. A DNA fingerprint is a pattern of bands made up of specific fragments from an individual’s DNA.
____ 9. Injection of a particular vaccine can cause the body to produce antibodies that protect against the possibility of
future infection by a particular pathogen.
____ 10. If a crop is made herbicide-resistant, treating it with an herbicide will seriously reduce its yield.

Multiple Choice
Identify the letter of the choice that best completes the statement or answers the question.

____ 11. Restriction enzymes are specific in their identification of

a. base sequences. c. proteins.
b. amino acids. d. chromosomes.
____ 12. Fragments of DNA having complementary “sticky ends”
a. are found only in bacterial cells.
b. can join with each other.
c. can join only with complementary fragments of the same species.
d. are immediately digested by enzymes in the cytoplasm of the cell.
____ 13. Plasmids
a. are circular pieces of bacterial DNA.
b. can replicate independently of the organism’s main chromosome.
c. are often used as vectors in genetic engineering experiments.
d. All of the above
____ 14. Cloning is a process by which
a. undesirable genes may be eliminated.
b. many identical DNA fragments are produced.
c. a virus and a bacterium may be fused into one.
d. many identical cells may be produced.
____ 15. Which of the following procedures is not a usual step in a gene transfer experiment?
a. inducing a mutation in a source chromosome
b. cleaving DNA with a restriction enzyme
c. recombining pieces of DNA from different organisms
 d. cloning and screening target cells
____ 16. A strand of DNA formed by the splicing of DNA from two different species is called
a. determinant RNA. c. plasmid DNA.
b. recombinant DNA. d. restriction RNA.
____ 17. DNA fingerprinting has been used in criminal investigations because
a. criminals leave DNA samples behind them when they touch objects at a crime scene.
b. DNA analysis is believed to allow investigators to distinguish body cells of different
individuals, who are unlikely to have the same DNA.
c. bacterial DNA on the hands of criminals may provide a clue as to where that person was
when the crime was committed.
d. DNA found on murder weapons is easy to identify.
____ 18. PCR and DNA replication
a. are used in genetic engineering to make copies of RNA.
b. require the same ingredients to make copies of DNA.
c. are used in genetic engineering to make proteins.
d. None of the above
____ 19. PCR : copies of DNA segments ::
a. genetically engineered bacterium : human proteins
b. genetic engineering : new genes
c. RNA polymerase : restriction enzymes
d. DNA plasmid : gel electrophoresis
____ 20. A genome is
a. an organism’s collection of genes.
b. a process used to copy DNA.
c. the nucleotide sequence that makes up a particular gene.
d. a fragment of DNA added to a chromosome during a gene transfer experiment.
____ 21. The goal of the Human Genome Project is to
a. create maps showing where genes are located on human chromosomes.
b. create maps showing where chromosomes are located on human genes.
c. treat patients with genetic diseases.
d. identify people with genetic diseases.
____ 22. Transferring normal human genes into human cells that lack them
a. is impossible at this time.
b. will cause cancer.
c. will cause antibodies to kill those cells.
d. is called gene therapy.
____ 23. Legumes are plants with nodules on their roots containing bacteria that convert N 2 gas into a form of nitrogen that
plants can use. To accomplish this conversion, the bacteria carry out a process called
a. genetic fixation. c. nitrogen fixation.
b. nitrogen plasmid formation. d. nitrogenous decomposition.
____ 24. Genetic engineering of crop plants to make them resistant to weed killers is important because
a. it increases erosion of topsoil.
b. many weed killers remain active for years.
c. weed killers also kill moth and butterfly larvae.
d. the cost of producing these crops is less since they do not need to be weeded.
____ 25. nitrogen-fixing bacteria : air ::
a. fertilizers : low-nitrogen fertilizer
b. crops : air
c. crops : high-nitrogen fertilizer
 d. genetically engineered crops : fertilizer

Completion
Complete each sentence or statement.

26. The set of thousands of DNA fragments produced by cutting a genome with restriction enzymes is called a(n)
____________________.
27. A technique known as ____________________ can be used to separate molecules in a mixture by subjecting
them to an electrical field within a gel.
28. Transferring normal human genes into human cells that lack them is called ____________________.
29. A(n) ____________________ is a preparation of killed or weakened pathogens that is introduced into the body to
produce immunity.
30. A major concern about genetically engineered foods is that they might contain substances that cause
____________________ in people who eat them.
Biology Test Chapter 13
Answer Section

TRUE/FALSE

1. ANS: T
2. ANS: T
3. ANS: F
4. ANS: T
5. ANS: F
6. ANS: T
7. ANS: T
8. ANS: T
9. ANS: T
10. ANS: F

MULTIPLE CHOICE

11. ANS: A
12. ANS: B
13. ANS: D
14. ANS: D
15. ANS: A
16. ANS: B
17. ANS: B
18. ANS: B
19. ANS: A
(makes)
20. ANS: A
21. ANS: A
22. ANS: D
23. ANS: C
24. ANS: D
25. ANS: C
(get nitrogen from)

COMPLETION

26. ANS: genomic library

27. ANS: gel electrophoresis
28. ANS: gene therapy
29. ANS: vaccine
30. ANS: allergies